Oligonucleotide sequences used for 16S sequence analysis
Gene detection with synthetic oligonucleotide sequences
... 4.6.2 M13 CLONING AND SEQUENCING OF PCR PRODUCTS Several attempts were made to clone the PCR products of genomic amplification using the short CG-rich oligos directly into vectors, in order to facilitate the sequencing ...
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Partial Transmit Sequence used in OFDM to Increase PAPR and Analysis of Phase Sequences and Data Blocks
... The high data rate transmission is the today’s most demanding parameter hence one of the popular technique used in the communication is OFDM. Partial transmit sequence (PTS) combining can improve the PAR ...
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reaction and 16S-rRNA gene sequences
... repetitive sequence-based polymerase chain reaction and 16S- rRNA gene ...Identification analysis was conducted with 16S-rRNA gene sequences followed by cluster analysis using ...
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Design and evaluation of 16S rRNA sequence based oligonucleotide probes for the detection and quantification of Comamonas testosteroni in mixed microbial communities
... Pure culture evaluation of the designed oligonucleotides All oligonucleotides were evaluated in situ on a selection of proteobacterial pure cultures including all C. testosteroni strains available from the German ...
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Phylogenetic Analysis of 16S rDNA Sequences of Pediococcus acidilactici TISTR 2309:
... phylogenetic analysis of 16S rDNA sequences by these 3 methods indicated that ...same sequence of TISTR 2309 was used as input for search tools of NCBI Blast and ENA, the two databases ...
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Fast comparison of DNA sequences by oligonucleotide profiling
... generate sequences expected to occur very rarely by chance, are ...source sequence and the program computes for that word a hash value: each of the nucleotides in a word is con- verted using a two-bits ...
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Bacterial 16S Sequence Analysis of Severe Caries in Young Permanent Teeth
... the 16S amplicons generated by PCR were as previously described ...the 16S hypervariable regions V5 to V9. (iv) Bacterial 16S rRNA gene sequence ...The sequence from each clone was ...
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Development of novel fluorescent oligonucleotide probes for use in nucleic acid sequence analysis
... The solvents were removed in vacuo and the resulting oil dissolved in CH2Ch 200mL, washed with water 200mL, saturated sodium bicarbonate solution 200mL, brine 150mL and then dried Na2S04[r] ...
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Sequence Textbook Sequences and Series
... This textbook was specifically used for a Coursera course called “Calculus Two: Sequences and Series.” Many thanks go to Walter Nugent, Donald Wayne Fincher, Robert Pohl, chas, Clark Archer, Mikhail, Sarah ...
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Comparative Phylogeny of the Genus Bordetella Using Sequence Analysis of 16S rRNA and ompA Genes
... uncultured sequence. Besides, sequence dataset of ompA gene was produced using the similarity search engines of BLAST program available at ...the 16S rRNA gene was the same. Thus, the 16S rRNA ...
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Signature region within the 16S rDNA sequences of Aeromonas popoffii
... Abstract To identify a group of eight Aeromonas strains of our collection showing ribotyping patterns similar to those described for the species Aeromonas popoffii, 16S rRNA gene sequence analysis ...
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Optimizing Copy Number Variation Analysis Using Genome-wide Short Sequence Oligonucleotide Arrays
... short sequence oligonucleotide array (Affymetrix) with a long sequence oligonucleotide CGH array custom designed for CNV detection ...the analysis pipeline can lead to overall FNR and ...
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Phylogeny of the Order Bacillales inferred from 3’ 16S rDNA and 5’ 16S 23S ITS nucleotide sequences
... the 16S rDNA and the 16S-23S Internal transcribed spacer (ITS) were determined for 32 Bacillales species and ...quence used here covers a conserved 150 bp sequence located at the 3’ end of the ...
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16S Ribosomal DNA Sequence Analysis of a Large Collection of Environmental and Clinical Unidentifiable Bacterial Isolates
... query sequence, we obtained similarity percentages using a gapped program applied to the entire 16S rDNA se- ...same sequence was compared against the same database using dif- ferent programs, ...
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16S Ribosomal DNA Sequence Based Analysis of Clinically Significant Gram Positive Anaerobic Cocci
... the 16S rRNA sequence of type strains of GPAC was to evaluate the accuracy of results obtained by programs such as BLAST and ...same sequence was compared against the same database (GenBank) by using ...
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Comparison of 16S ribosomal RNA gene sequence analysis and conventional culture in the environmental survey of a hospital
... the 16S rRNA gene se- quencing analysis identified as ...hand, 16S rRNA gene sequence analysis has been used to identify novel and emerging pathogens and to define complex ...
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Bacterial community dynamics in a swine wastewater anaerobic reactor revealed by 16S rDNA sequence analysis
... Advances in methodologies are necessary to obtain a more detailed understanding of these complex communities, which is fundamen- tal for the improvement of digester efficiency and stability. In this study, high-throughput ...
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Identification and Analysis of Informative Single Nucleotide Polymorphisms in 16S rRNA Gene Sequences of the Bacillus cereus Group
... c Analysis of 16S rRNA genes is important for phylogenetic classification of known and novel bacterial genera and species and for detection of uncultivable ...of 16S rRNA genes with universal primers ...
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tuf Gene Sequence Analysis Has Greater Discriminatory Power than 16S rRNA Sequence Analysis in Identification of Clinical Isolates of Coagulase Negative Staphylococci
... terial 16S rRNA sequence data, including data for staphylo- cocci, related Gram-positive cocci, and ...gene analysis and aimed to determine the optimal criteria for CNS species identification by tuf ...
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Prevalence of Corynebacterial 16S rRNA Sequences in Patients with Bacterial and “Nonbacterial” Prostatitis
... rDNA sequence data (compare Table 1 with Table 2). For example, the 16S rDNAs predominately obtained from patients B and C corresponded to those of Streptococcus ...only 16S rDNAs indicative of ...
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