PCR-single-strand conformation polymorphism
Rapid Identification of Bacteria from Positive Blood Cultures by Fluorescence Based PCR–Single Strand Conformation Polymorphism Analysis of the 16S rRNA Gene
... Bacteremia continues to result in significant morbidity and mortality, particularly in patients who are immunocompromised. Currently, patients with suspected bacteremia are empirically administered broad- spectrum ...
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Molecular identification of bacteria by fluorescence based PCR single strand conformation polymorphism analysis of the 16S rRNA gene
... PCR–single-strand conformation polymorphism (PCR-SSCP) analysis is a rapid and convenient technique for the detection of mutations and allelic ...by PCR with ...
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Identification of Mycobacterium Species by Multiple Fluorescence PCR–Single Strand Conformation Polymorphism Analysis of the 16S rRNA Gene
... SSCP electrophoresis. SSCP electrophoresis was performed with the ABI PRISM 310 Genetic Analyzer (PE Applied Biosystems, Foster City, Calif.). The two PCR mixtures were run in two separate sample tubes. The ...
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Simple Method for Determining Biovar and Serovar Types of Ureaplasma urealyticum Clinical Isolates Using PCR–Single Strand Conformation Polymorphism Analysis
... a PCR product of 173 bp from which three conformer band patterns were ob- tained; serovars 1 and 6 gave distinct patterns, and those of serovars 3 and 14 were ...1. PCR-SSCP patterns of the reference ...
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Characterization of Salmonella Serovars by PCR Single Strand Conformation Polymorphism Analysis
... is PCR–single-strand con- formation polymorphism (PCR-SSCP) (6, ...to single-stranded DNA and subjected to nondenaturing poly- acrylamide gel ...the single- stranded DNA ...
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Capillary Electrophoresis–Single Strand Conformation Polymorphism Analysis for Rapid Identification of Pseudomonas aeruginosa and Other Gram Negative Nonfermenting Bacilli Recovered from Patients with Cystic Fibrosis
... from GENSET SA (Paris, France), were used for amplification of the 16S rRNA gene regions. The first set of primers was P11P (5⬘-GAG GAA GGT GGG GAT GAC GT) and P13P (5⬘-AGG CCC GGG AAC GTA TTC AC), to amplify a 217-bp ...
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PCR and single strand conformational polymorphism for recognition of medically important opportunistic fungi
... of PCR technology to molecular diagnostics holds great promise for the early identification of medically important ...pathogens. PCR has been shown to be useful for the detection of the presence of fungal ...
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Genotypic detection of Mycobacterium tuberculosis rifampin resistance: comparison of single strand conformation polymorphism and dideoxy fingerprinting
... Cost analysis of ddF versus automated DNA sequencing. The approximate material cost and time commitment for doing a ddF reaction (5 9 -end fluorescein- labelled primer, with products analyzed by using an automated ...
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Application of biological and single-strand conformation polymorphism assays for characterizing potential mild isolates of Citrus tristeza virus for cross protection
... Coat protein p25 gene fragments of CTV were used for SSCP analysis. 9 µL of denaturing solution (20 mM EDTA pH 8.0, 95% v/v deionized Formamide, 0.25% w/v Bromophenol blue and 0.25% Xylene-cyanol), was mixed with 1 µL of ...
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Detection of rifampin resistance by single strand conformation polymorphism analysis of cerebrospinal fluid of patients with tuberculosis of the central nervous system
... FIG. 2. PCR-SSCP patterns obtained by processing CSF samples from pa- tients with CNS-TB. Lanes S, pattern obtained from rifampin-sensitive M. tu- berculosis reference strain (ATCC 27294). Samples (lanes) 1 to 3, ...
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Typing of Pneumocystis carinii f sp hominis by single strand conformation polymorphism of four genomic regions
... (Mycrosynth, Balgach, Switzerland), annealing temperature, magnesium concen- tration, and pH used for each PCR are given in Table 1. The 39 primer used for amplification of the intron of the nuclear 26S rRNA gene ...
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Rapid Screening of Fluoroquinolone Resistance Determinants in Streptococcus pneumoniae by PCR Restriction Fragment Length Polymorphism and Single Strand Conformational Polymorphism
... using PCR-restriction fragment length and single-strand conformation polymorphism (SSCP), was applied to screen for mutations of the fluoroquinolone resistance determinants in ...
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Multiple infections of Ixodes scapularis ticks by Borrelia burgdorferi as revealed by single strand conformation polymorphism analysis
... by PCR (10). Genetic variation was then sur- veyed at this locus by single-strand conformation polymor- phism (SSCP) analysis, a technique which distinguishes short, fixed-length DNA fragments ...
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Genetic Diversity among Borrelia Strains Determined by Single Strand Conformation Polymorphism Analysis of the ospC Gene and Its Association with Invasiveness
... the PCR product obtained in repeated experiments from a given DNA always yielded the same SSCP pattern and one PCR product always yielded the same SSCP pattern in different ...
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DETECTION SINGLE NUCLEOTIDE POLYMORPHISMS IN UROMODULIN PROMOTER REGION ASSOCIATED WITH RENAL DISEASES USING SINGLE STRAND CONFORMATION POLYMORPHISM POLYMERASE CHAIN POLYMORPHISMS TECHNIQUE
... PCR was carried out in the reaction mixture containing 1 µl from forward and reverse primers, 12.5 µl of Green Master Mix, and 3 µl of genomic DNA, and the volume of reaction was completed up to 20 µl by adding ...
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Nested PCR Linked Capillary Electrophoresis and Single Strand Conformation Polymorphisms for Detection of Macrolide Resistant Mycoplasma pneumoniae in Beijing, China
... of PCR products, re- striction fragment length polymorphism (RFLP) analysis, or other mutation detection methods, such as real-time PCR and high-resolution melt analysis (25, 33, ...The ...
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Detection of Rifampin Resistant Mycobacterium tuberculosis in Sputa by Nested PCR Linked Single Strand Conformation Polymorphism and DNA Sequencing
... Conventional PCR-linked SSCP and DNA sequencing. Conventional PCR- SSCP was performed as previously described, using TR9 and TR8 primers (12, ...conventional PCR-DNA ...purified PCR product ...
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A single strand conformation polymorphism study of CD40 ligand Efficient mutation analysis and carrier detection for X linked hyper IgM syndrome
... Mutations in the gene for CD40 ligand are responsible for the X-linked form of hyper IgM syndrome. However, no clinical or laboratory findings that reliably distinguish X-linked dis- ease from other forms of hyper IgM ...
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Study on DGAT1-exon8 Polymorphism in Iranian Buffalo
... the polymorphism in exon 8 of the DGAT1 gene in Iranian buffaloes using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) that allows the ...
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Novel Algorithm Identifies Species in a Polymycobacterial Sample by Fluorescence Capillary Electrophoresis Based Single Strand Conformation Polymorphism Analysis
... (FCE)-based single-strand conformation polymorphism (SSCP) analysis was ...was PCR amplified with a fluorescence-labeled mycobacterium-specific primer, 6-carboxyfluorescein-labeled ...
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