Peptide-Nucleic Acid
Separation of Mycobacterium abscessus into subspecies or genotype level by direct application of peptide nucleic acid multi probe real time PCR method into sputa samples
... Peptide nucleic acids (PNAs) are artificially synthesized DNA analogues with an uncharged peptide ...and peptide bond-linked backbone ...novel peptide nucleic acid (PNA) ...
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Rapid Identification of Staphylococcus aureus Directly from Blood Cultures by Fluorescence In Situ Hybridization with Peptide Nucleic Acid Probes
... with peptide nucleic acid (PNA) probes for identification of Staphylococcus aureus directly from positive blood culture bottles that contain gram-positive cocci in clusters (GPCC) is ...
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Development and Application of a Novel Peptide Nucleic Acid Probe for the Specific Detection of Helicobacter pylori in Gastric Biopsy Specimens
... detects nucleic acid sequences by means of a fluorescently labeled probe that hybridizes specifically to its complementary target sequence within the intact cell ...recently, peptide nucleic ...
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Identification of Fusarium Species in Formalin Fixed and Paraffin Embedded Sections by In Situ Hybridization Using Peptide Nucleic Acid Probes
... Fusarium has recently emerged as an opportunistic pathogen of humans, but the histological differentiation of Fusarium from Aspergillus and Scedosporium is particularly difficult because these fungi may induce similar ...
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Design and Evaluation of Peptide Nucleic Acid Probes for Specific Identification of Candida albicans
... Peptide nucleic acid (PNA) probes are synthetic DNA mimics with improved performance characteristics compared to DNA probes. Specifically, these characteristics include faster hybridiza- tion ...
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Fluorescence In Situ Hybridization with Peptide Nucleic Acid Probes for Rapid Identification of Candida albicans Directly from Blood Culture Bottles
... uses peptide nucleic acid (PNA) probes for identification of Candida albicans directly from positive-blood-culture bottles in which yeast was observed by Gram staining (herein referred to as ...
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Fluorescence In Situ Hybridization Assay Using Peptide Nucleic Acid Probes for Differentiation between Tuberculous and Nontuberculous Mycobacterium Species in Smears of Mycobacterium Cultures
... using peptide nucleic acid (PNA) probes for differentiation between species of the Mycobacterium tuberculosis complex (MTC) and nontubercu- lous mycobacteria (NTM) in acid-fast ...
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Evaluation of Peptide Nucleic Acid Fluorescence In Situ Hybridization for Identification of Clinically Relevant Mycobacteria in Clinical Specimens and Tissue Sections
... for acid-fast bacilli from a variety of clinical ...upon nucleic acid amplification (Roche COBAS Amplicor for members of the ...oleic acid-albumin-dextrose-citric acid) and an anti- ...
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Peptide Nucleic Acid Array for Detection of Point Mutations in Hepatitis B Virus Associated with Antiviral Resistance
... The detection of antiviral-resistant hepatitis B virus (HBV) mutations is important for monitoring the response to treatment and for effective treatment decisions. We have developed an array using peptide ...
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A Peptide Nucleic Acid to Reduce Type I Collagen Production by Fibroblast Cells
... Reagents for PNA synthesis were purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan) unless otherwise noted. PNA oligomers were manually synthesized from commercial PNA monomers (PolyOrg, Leo- minster, MA, ...
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Multicenter Evaluation of the Staphylococcus QuickFISH Method for Simultaneous Identification of Staphylococcus aureus and Coagulase Negative Staphylococci Directly from Blood Culture Bottles in Less than 30 Minutes
... There are a number of methods currently used for identifica- tion of staphylococci in clinical samples, and they vary greatly in accuracy, speed, and cost. The tube coagulase test detects the pres- ence of free coagulase ...
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Peptide Nucleic Acid Based Array for Detecting and Genotyping Human Papillomaviruses
... We describe a novel array for accurate and reliable genotyping of human papillomavirus (HPV) using peptide nucleic acid (PNA) probes. In order to exploit the superior hybridization properties of PNA ...
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Algorithms for nucleic acid sequence design
... thermodynamic nucleic acid design a high-quality, versatile, and low-cost technology that is easy to use and easily ...of nucleic acids that also achieves high-quality sequence for low cost with ...
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Search for a Prion-Specific Nucleic Acid
... inoculation, we reassessed the particle to infectivity (P/I) ratio and the maximum possible size of a prion-specific polynucle- otide. We found that polynucleotides larger than 25 bases were not in sufficient abundance ...
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Whole body composition of Xenopus laevis larvae: implications for lean body mass during development
... larval Ambystoma tigrinum, in which DNA content was linearly correlated with wet mass (A. W. Smits, unpublished data). Although few studies exist which have correlated nucleic acid content with wet or dry ...
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Immunogenicity of porcine circovirus type 2 nucleic acid vaccine containing CpG motif for mice
... Nucleic acid vaccine is one of the hot spot of PCV2 vaccine ...PCV2 Nucleic acid vaccine was first re- ported by Kamstrup [16], they developed and investigated the potential of DNA vaccination ...
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NUCLEIC ACIDS Centrifugation pdf
... The recent explosion of information from recom- binant DNA technology and the human genome initi- ative has come about in response to a number of key technological advances. These include the discovery and ...
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Functional nucleic acid probes and uses thereof
... functional nucleic acid probes, and methods of using functional nucleic acid probes, for binding a target to carry out a desired ...functional nucleic acid, at least one ...
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Functional nucleic acid probes and uses thereof
... functional nucleic acid probes, and methods of using functional nucleic acid probes, for binding a target to carry out a desired ...functional nucleic acid, at least one ...
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Establishment of national reference for bunyavirus nucleic acid detection kits for diagnosis of SFTS virus
... Nucleic acid detection kits for SFTSV (PCR-fluores- cence probe techniques) were obtained from SinoMD Gene (Beijing, China) and DaAn Gene (Guangzhou, ...
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