real-time quantitative PCR
Development of Conventional and Real Time Quantitative PCR Assays for Diagnosis and Monitoring of Scabies
... nested PCR on a small number of cases, no systematic study has been performed to improve the laboratory diagnosis of this important ...a real-time quantitative PCR (qPCR) assay based on ...
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Real Time Quantitative PCR Detection of Four Human Bocaviruses
... Human bocavirus (HBoV) was discovered in 2005 and is associated with respiratory tract symptoms in young children. Three additional members of the genus Bocavirus, HBoV2, -3, and -4, were discovered recently from fecal ...
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Simultaneous Detection of Influenza Viruses A and B Using Real Time Quantitative PCR
... 25-l PCR was performed using 5 l of cDNA, 12.5 l of TaqMan universal PCR master mix containing ROX as a passive reference (PE Applied Biosystems), 900 nM each influenza virus A primer, 300 nM each ...
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Comparison of Conventional, Nested, and Real Time Quantitative PCR for Diagnosis of Scrub Typhus
... nested PCR (N-PCR) is widely used to improve the sensitivity of conventional PCR (C-PCR) (3, 4, ...addition, real-time quantitative PCR (Q-PCR) targeting a ...
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Development of a Real Time Quantitative PCR for Detecting Duck Hepatitis A Virus Genotype C
... by real-time PCR was performed in a 20- l reaction mixture containing ...7300 Real Time PCR system (ABI). The PCR conditions consisted of one cycle of 3 min at 95°C ...
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Real Time Quantitative PCR To Determine Chlamydial Load in Men and Women in a Community Setting
... over time among women and men who had specimens for both time points, using paired t ...both time points, we used the value for the first ...over time, but this did not affect the results ...
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Quantitative Assessment of WT1 Expression by Real Time Quantitative PCR in Pediatric Patients with Acute Myeloblastic Leukemia
... GAC PCR buffer 1X contained 10mM Tris-HCl, 50 mM KCl (pH ...and time conditions are described as the following: Initial melting 95°C for 30 s, 35 PCR cycles with the following conditions: 94°C for 30 ...
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Time-dependent post mortem changes in the composition of intestinal bacteria using real-time quantitative PCR
... mortem time (Figure 1). In cecum, significant post mortem time-dependent differences were observed over the groups in the relative amounts of Bacteroides ...with time elapsing post mortem except for ...
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Real Time Quantitative PCR for Detection of Helicobacter pylori
... The most striking result of our examination of clinical biopsy samples was that many that were negative for H. pylori by culture contained large amounts of H. pylori DNA when ana- lyzed by Q-PCR. A possible ...
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Analysis of ZAP70 expression in adult acute lymphoblastic leukaemia by real time quantitative PCR
... While several groups, including our own, have demon- strated ZAP70 mRNA expression in paediatric pre-B ALL [8], there have been no previous studies that have used RT-qPCR to determine ZAP70 expression in adult cases of ...
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Real Time Quantitative PCR for Human Herpesvirus 6 DNA
... Quantitative-competitive PCR (QC-PCR) was performed with a fixed input of 1,000 copies of the synthetic competitor DNA molecule of 96 bp described above per reaction ...1⫻ PCR buffer II (PE ...
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Development of a Quantitative Real Time PCR Assay for Detection of Mycoplasma genitalium
... the PCR was initiated. UNG destroys PCR products containing ...the PCR reagents were mixed in a PCR clean room, and the template samples were loaded with filter ...for real-time ...
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Multiplex, Quantitative, Real Time PCR Assay for Cytomegalovirus and Human DNA
... of real-time PCR technology has simpli- fied nucleic acid quantification and is coming into more wide- spread ...use. Real-time, quantitative PCR has several advan- tages ...
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Real Time PCR for Quantitative Detection ofToxoplasma gondii
... Several PCR-based techniques (16, 18, 24) have been devel- oped for the diagnosis of toxoplasmosis using various clinical specimens, including amniotic fluid (3, 11), blood (1, 13, 17), cerebrospinal fluid (27), ...
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Validation and implementation of hepatitis b viral load detection with quantitative real time PCR
... reproducibility. Quantitative HBV DNA measurements for specimens with viral load assay showed excellent correlation with Digital PCR results ...of PCR inhibitors. Digital PCR can be used to ...
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Development and evaluation of a real time PCR assay for the quantitative detection of Theileria annulata in cattle
... Real-time quantitative PCR was performed in Micro- Amp TM Fast optical 96-well reaction plates covered with thermo-sealing 4titude Clear Seal adhesives (Surrey, UK) at 180°C during 2 seconds ...
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Quantitative Analysis of Epstein Barr Virus Load by Using a Real Time PCR Assay
... novel real-time quantitative PCR was developed (7, ...of PCR prod- ucts with a fluorogenic probe and by real-time laser scanning in a 96-well ...the ...
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Implementation of an In House Quantitative Real Time PCR for Determination of HIV Viral Load in Kinshasa
... other real-time thermal cycler that has been previously ...The PCR can be run on any Real-Time thermal cycler that can observe the protocols of temperature and ...
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Real-Time PCR Vs. Traditional PCR
... The Amplification Plot contains valuable information for the quantitative measurement of DNA or RNA. The Threshold line is the level of detection or the point at which a reaction reaches a fluorescent intensity ...
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Quantitative Detection of Borrelia burgdorferi by Real Time PCR
... performed PCR assays, we decided to exploit the recently developed real-time ...linear quantitative detection range of 5 logs with a calculated detection limit of one to three spirochetes per ...
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