Reverse line blot (RLB)
Simultaneous Identification of 14 Genital Microorganisms in Urine by Use of a Multiplex PCR Based Reverse Line Blot Assay
... PCR-based reverse line blot (mPCR/RLB) assay was developed to detect 14 urogenital pathogens or putative pathogens, namely Trichomonas vaginalis, Streptococcus pneu- moniae, Neisseria ...
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Use of PCR and Reverse Line Blot Hybridization Assay for Rapid Simultaneous Detection and Serovar Identification of Chlamydia trachomatis
... infections have been reported to involve two or more (1–3, 7,13–16). IF and EIA are commonly used for serotyping and detection of multiple serotypes in C. trachomatis cultures. PCR, plus restriction fragment length ...
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Reverse Line Blot Assay for Direct Identification of Seven Streptococcus agalactiae Major Surface Protein Antigen Genes
... (mPCR)-based reverse line blot (RLB) hybridization assay and used it to identify seven protein antigen genes in several sets of well-character- ized GBS ...
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Simultaneous Detection of BovineTheileria and Babesia Species by Reverse Line Blot Hybridization
... A reverse line blot (RLB) technique fulfilling these criteria has recently been developed to detect four different Borrelia species in ticks ...(39). RLB was initially developed as a ...
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Comparative study of bacteriological culture and real-time fluorescence quantitative PCR (RT-PCR) and multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay in the diagnosis of bacterial neonatal meningitis
... based-reverse line blot hybridization (mPCR/RLB), were used to assess 7 common neonatal meningitis bacterial pathongens, including Escherichia coli, Staphylococcus aureus, Listerisa ...
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Simultaneous Detection and Identification of Candida, Aspergillus, and Cryptococcus Species by Reverse Line Blot Hybridization
... Reverse line blot hybridization assay. The RLB hybridization assay was mod- ified (31), based on a previously described method (29). In brief, the nylon membrane-bound oligonucleotide probes ...
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Detection and Identification of EightTrichinella Genotypes by Reverse Line Blot Hybridization
... A reverse line blot (RLB) assay was developed to identify different Trichinella ...The RLB assay accomplishes detection and specific identification of the different Trichinella ...
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Molecular Diagnosis of Old World Cutaneous Leishmaniasis and Species Identification by Use of a Reverse Line Blot Hybridization Assay
... Reverse line blot hybridization assays (RLB) have been used for the rapid diagnosis and genotyping of many ...the RLB is 10- to 100-fold more sensitive than ITS1 PCR and can detect ...
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New Method for Simultaneous Species Specific Identification of Equine Strongyles (Nematoda, Strongylida) by Reverse Line Blot Hybridization
... a reverse line blot (RLB) assay to identify 13 common species of equine small strongyles (cyathostomins) and to discriminate them from three Strongylus ...this RLB method promises to be ...
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Identification of 20 Common Human Enterovirus Serotypes by Use of a Reverse Transcription PCR Based Reverse Line Blot Hybridization Assay
... on reverse transcription-PCR (RT-PCR) amplification and nucleotide sequencing of the entire or 3 ⴕ half of the VP1 ...RT-PCR-based reverse line blot (RLB) hybridization assay was ...
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Identification of Less Common Streptococcus pneumoniae Serotypes by a Multiplex PCR Based Reverse Line Blot Hybridization Assay
... PCR-based reverse line blot (mPCR/RLB) hybridization assay to identify 40 Strepto- coccus pneumoniae serotypes, including the 23 represented in the polysaccharide vaccine and 17 others that ...
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Reverse Line Blot Hybridization Assay for Identification of Medically Important Fungi from Culture and Clinical Specimens
... PCR-reverse line blot (RLB) hybridization assay based on internal transcribed spacer 1 (ITS1) and ITS2 region polymorphisms to identify 159 Candida, Cryptococcus neoformans, and Aspergillus ...
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Rapid Diagnosis of Extensively Drug Resistant Tuberculosis by Use of a Reverse Line Blot Hybridization Assay
... The reverse line blot hybridization (RLBH) assay (11, 24) is a probe-based method where multiple oligonucleotide probes carrying the mutant sequence and wild-type sequence are im- mobilized on ...
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Performance of Commercial Reverse Line Blot Assays for Human Papillomavirus Genotyping
... Reverse line blot assays (LBAs), based on consensus amplifica- tion of conserved regions of HPV followed by hybridization to type-specific probes on line blot strips, have been widely ...
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Replacing Reverse Line Blot Hybridization Spoligotyping of the Mycobacterium tuberculosis Complex
... membrane-based reverse line blot hybridization pro- cess involves four steps, consisting of PCR target amplification, hybridization, posthybridization wash steps, and chemilumi- nescent ...
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Simultaneous Detection and Genotyping of “Norwalk Like Viruses” by Oligonucleotide Array in a Reverse Line Blot Hybridization Format
... of reverse transcription (RT)-PCR products, which has revealed the existence of stable distinct lineages ...designated reverse line blot hybridization, is based on the nucleotide divergence of ...
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Multiplex PCR Based Reverse Line Blot Hybridization Assay To Identify 23 Streptococcus pneumoniae Polysaccharide Vaccine Serotypes
... FIG. 1. mPCR/RLB results for a representative sample of 43 clinical isolates. See Table 1 for descriptions and specificities of probes listed at left. The choice of probes for this membrane, in addition to two ...
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GP5+/6+ PCR followed by Reverse Line Blot Analysis Enables Rapid and High Throughput Identification of Human Papillomavirus Genotypes
... of RLB detection was identical to that of EIA ...systems. RLB and EIA reached the same detection level, and the sensitivities for these HPV types were identical to those previously de- scribed for EIA (8), ...
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Detection of Rickettsia in Rhipicephalus sanguineus Ticks and Ctenocephalides felis Fleas from Southeastern Tunisia by Reverse Line Blot Assay
... amino linker extremity were carried out as previously described (7). Positive controls were plasmids containing the 23S-5S intergenic spacer sequence from R. prowazekii, R. conorii, and R. aeschlimannii, kindly provided ...
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The substructure of three repetitive DNA regions of Schistosoma haematobium group species as a potential marker for species recognition and interbreeding detection
... The newly identified diverse regions (the Sh64bp and Sb30bp repeat linkers) were further developed into a one-step PCR assay with an aim to discriminate between S. haematobium and S. bovis. As a result, the DNA se- ...
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