Reverse transcriptases
Evolutionarily Conserved Epitopes on Human Immunodeficiency Virus Type 1 (HIV-1) and Feline Immunodeficiency Virus Reverse Transcriptases Detected by HIV-1-Infected Subjects
... (FIV) reverse transcriptases (RT), were identified using gamma in- terferon (IFN-␥) enzyme-linked immunosorbent spot (ELISpot) and carboxyfluorescein diacetate succinimide ester (CFSE) pro- liferation ...
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Biotechnological applications of mobile group II introns and their reverse transcriptases: gene targeting, RNA-seq, and non-coding RNA analysis
... intron-encoded reverse transcriptase to insert site-specifically into ...intron reverse transcriptases that yield large amounts of highly active protein, thermostable group II intron reverse ...
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Phenotypic and Genotypic Comparisons of Human T-Cell Leukemia Virus Type 1 Reverse Transcriptases from Infected T-Cell Lines and Patient Samples
... between reverse transcriptase sequence and the disease phenotype of the original source of the virus ...encoding reverse transcriptase were amplified from a variety of isolates and were subcloned into ...
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When retroviral reverse transcriptases reach the end of their RNA templates.
... When an RNA template is acted upon by such a reverse transcriptase, there is both the synthesis of a DNA product by the polymerase activity and concomitant endonucleolytic cleavage of th[r] ...
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Crystal Structures of Zidovudine- or Lamivudine-Resistant Human Immunodeficiency Virus Type 1 Reverse Transcriptases Containing Mutations at Codons 41, 184, and 215
... 7. Jacobo-Molina, A., J. P. Ding, R. G. Nanni, A. D. J. Clark, X. Lu, C. Tantillo, R. L. Williams, G. Kamer, A. L. Ferris, P. Clark, A. Hizi, S. H. Hughes, and E. Arnold. 1993. Crystal structure of human immunodeficiency ...
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Wild-Type and YMDD Mutant Murine Leukemia Virus Reverse Transcriptases Are Resistant to 2′,3′-Dideoxy-3′-Thiacytidine
... The antiretroviral nucleoside analog 2 ⴕ ,3 ⴕ -dideoxy-3 ⴕ -thiacytidine (3TC) is a potent inhibitor of wild-type human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT). A methionine-to-valine or ...
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Kinetic variations between reverse transcriptases of viral protein X coding and noncoding lentiviruses
... Interestingly, unlike HIV-1, HIV-2 replicates more rapidly in nondividing cells [12]. This phenotype is directly linked to a viral accessory protein, called viral protein X (Vpx), which is encoded by HIV-2 and many SIV ...
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Interspecies antigenic determinants of the reverse transcriptases and p30 proteins of mammalian type C viruses.
... type C viruses studied Characterization NB-tropic leukemogenic virus propagated in BALB/c JLSV9 cells N-tropic virus spontaneously released from BALB/c S2CL3 55 S2CL3 cell line B-tropic [r] ...
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Relaxed Primer Specificity Associated with Reverse Transcriptases Encoded by the pFOXC Retroplasmids of Fusarium oxysporum
... The pFOXC RT uses minimally base-paired primers more readily than conventional RTs do. Based on the findings that the pFOXC RT can extend minimally base-paired, snapped- back RNAs and unstable primer duplexes, we ...
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HIV-1 and HIV-2 Reverse Transcriptases: Different Mechanisms of Resistance to Nucleoside Reverse Transcriptase Inhibitors
... nucleoside reverse trans- criptase inhibitors ...HIV-1 reverse transcriptase (RT) usually uses an ATP-de- pendent excision pathway to develop resistance to the nucleoside analog zidovudine (AZT), HIV-2 RT ...
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Biophysical and enzymatic properties of the simian and prototype foamy virus reverse transcriptases
... Background: The foamy virus Pol protein is translated independently from Gag using a separate mRNA. Thus, in contrast to orthoretroviruses no Gag-Pol precursor protein is synthesized. Only the integrase domain is cleaved ...
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Immunological Relationships of Reverse Transcriptases from Ribonucleic Acid Tumor Viruses
... Since antisera to the R-MuLV reverse transcriptase were equally potent whether tested against disrupted whole virus or partially purified enzyme, lack of inhibition of the reverse transc[r] ...
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Influence of the RNase H domain of retroviral reverse transcriptases on the metal specificity and substrate selection of their polymerase domains
... The chimeric HIV-1 RT and MuLV RT were constructed by swapping the RNase H domain between the reverse tran- scriptases from HIV-1 and MuLV (Figure 1A). The wild- type enzymes and their chimeric derivatives were ...
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Simple affinity procedure for the purification of mammalian viral reverse transcriptases.
... In the third affinity procedure, the reverse transcriptase in a nucleic acid-free viral extract was incubated in the cold with 50 ,uM polyguanylic acid and the complex was adsorbed onto [r] ...
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Cross-Clade Inhibition of Recombinant Human Immunodeficiency Virus Type 1 (HIV-1), HIV-2, and Simian Immunodeficiency Virus SIVcpz Reverse Transcriptases by RNA Pseudoknot Aptamers
... Reverse transcriptase (RT) remains a primary target in therapies directed at human immunodeficiency virus type 1 (HIV-1). RNA aptamers that bind RT from HIV-1 subtype B have been shown to protect human cells from ...
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Hepatitis B virus particles contain a polypeptide encoded by the largest open reading frame: a putative reverse transcriptase.
... In addition, the sequence similarity of the ORF P region used in the tribrid fusion protein to the other hepadnaviruses and the reverse transcriptases of retroviruses suggest that the an[r] ...
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Activation and characterization of the reovirus transcriptase: genetic analysis.
... Since the transcriptases from types 1 and 3 displayed the greatest differences in the conditions needed for activation, we used intertypic recombinants reassortants derived from crosses [r] ...
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The reverse DTML-conversion model with its mathematical formulas
... The Reverse DTML-Conversion model was designed by the researcher from the premise that learning to convert between units of measurement is critical to learners development in the realm of science and other courses ...
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2004 pdf
... 다음으로 파일 변조 분석을 위해 일반적으로 rootkit이 많이 설치되는 /dev 파일 조사를 통해 rootkit을 찾아내어 파일 생성 날짜와 rootkit 파일 분석을 중심으로 침해사고 분석을 진행 함... 다음으로 파일 변조 분석을 위해 일반적으로 rootkit이 많이 설치되는 /dev 파일 조사를 통해 rootkit을 찾아내어[r] ...
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100 pdf
... 즉, 저작물의 성립요건을 모두 갖추어 저작물로 성립하였다고 하더라도 그 저작물을 이루는 여러 가지의 구성요소 모두가 저작권법의 보호대상이 되는 것은 아니며, 저작권법의 보호 가 미치는 부분은 말, 문자, 음, 색 등에 의하여 구체적으로 외부에 표현한 창작적인 표현형식에 한정되고, 그 표현되어 있는 내용, 즉 아이디어나 이론 등의 사[r] ...
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