RNA expression profile
Circular RNA expression profile in the spinal cord of morphine tolerated rats and screen of putative key circRNAs
... In our previous research, we have already screened a great number of mRNAs that were greatly changed in the identical spinal cord samples [12]. By computative ceRNA analysis utilizing the validated DEcircRNAs, all of ...
10
Long noncoding RNA expression profile in fibroblast like synoviocytes from patients with rheumatoid arthritis
... Gene Expression Analysis protocol (Agilent Technology). Briefly, RNA was purified using the RNeasy Mini Kit (Qiagen, ...cRNA. RNA quantity and quality were measured according to the A260 nm/A280 nm ...
10
Long non coding RNA expression profile in minor salivary gland of primary Sjögren’s syndrome
... lncRNA expression levels and the clinical ...lncRNA expression levels; (2) visual analogue scale (VAS) of parotid swelling with two lncRNAs; (3) VAS of dry eyes with one lncRNA; (4) β2 microglobulin with ...
14
Long non coding RNA expression profile in human gastric cancer and its clinical significances
... hepatocarcinoma and prostate cancer. These results fur- ther certify that H19 acts not only as an oncogene but also as a tumor suppressor [20,27,33]. Matouk et al. found that the knocking-down of H19 RNA resulted ...
10
Original Article Identification of long non-coding RNA expression profile in tissue and serum of papillary thyroid carcinoma
... noncoding RNA gene PTCSC2 (papillary thyroid cancer susceptibility candidate 2) was down- regulated in PTC tumors ...lncRNA expression profile in PTC, H19, HOTAIR, MALAT1 and GAS1 were ...MALAT1 ...
9
Long noncoding RNA expression profile analysis of colorectal cancer and metastatic lymph node based on microarray data
... Gene Expression 8x60K v2 Microarray (Agilent Technolo- gies, Santa Clara, CA, ...specific expression of mRNAs and lncRNAs were defined when the absolute value of fold change was ...
14
Identification of micro RNA expression profile related to recurrence in women with ESMO low risk endometrial cancer
... the RNA, including the small micro-RNA fraction, was eluted with 20 μl dis- tilled ...the RNA recovered were measured using the Nanodrop 1000A spectrophotometer (Nanodrop Technologies, Wilming- ton, ...
13
Original Article Long noncoding RNA expression profile of endothelial progenitor cells from deep vein thrombosis patients identified by microarray analysis
... Here we demonstrated that TCONS_000135- 36, ENST00000577218, ENST00000511042 and TCONS_00013917 were upregulated and ENST00000544704 was downregulated in EPCs from DVT samples compared with con- trols. Furthermore, ...
12
Original Article Long non-coding RNA expression profile in atrial fibrillation
... total RNA was used to generate Cyanine-3 labeled cRNA, according to the Agilent One-Color Microarray- Based Gene Expression Analysis Low for Input Quick Amp Labeling kit ...
10
A subgroup of pancreatic adenocarcinoma is sensitive to the 5-aza-dC DNA methyltransferase inhibitor
... 5-aza-dC is a DNMT inhibitor incorporated into DNA as a deoxycytidine analog that forms irreversible covalent bonds with DNMT at cytosine sites targeted for methylation [9]. 5-aza-dC demonstrates activity against ...
9
Peripheral blood B lymphocytes derived from patients with idiopathic pulmonary arterial hypertension express a different RNA pattern compared with healthy controls: a cross sectional study
... assessing RNA- expression profiles of peripheral blood mononuclear cells by microarrays was introduced and shown to be able to differentiate variously classified pulmonary arterial hyper- tension patients ...
7
Enhanced expression of long non coding RNA NEAT1 is associated with the progression of gastric adenocarcinomas
... the expression of NEAT1 lncRNA in GACs and used a large number of cases in the current study to find a novel correlation between el- evated expression of NEAT1 lncRNA and GAC patho- ...higher ...
6
The effects of short-term high-fat feeding on exercise capacity: multi-tissue transcriptome changes by RNA sequencing analysis
... creased expression of the two genes suggested disturbed neuronal migration in HFD-fed mice, which may result in abnormal development of the nervous ...decreased expression in HFD-fed ...
12
Original Article Analysis of mRNA profile in different strategy of bladder cancer carcinoma by deep sequencing
... gene expression struc- ture, and these abnormal expressions of genes often promoted the incidence and develop- ment, malignant transformation, invasion and differentiation of the tumor, as well as deter- mined the ...
9
Doubly Spliced RNA of Hepatitis B Virus Suppresses Viral Transcription via TATA-Binding Protein and Induces Stress Granule Assembly
... the expression plasmids for 24 h and fixed in 4% paraformaldehyde, followed by an overnight incubation in 70% ethanol at ...labeled RNA was detected using a sheep anti- DIG antibody (Thermo Scientific), and ...
14
Aberrant modulation of the BRCA1 and G1/S cell cycle pathways in alcoholic hepatitis patients with Mallory Denk Bodies revealed by RNA sequencing
... for RNA-Seq were prepared with Nugen Ovation Human FFPE RNA-Seq Multiplex ...gene expression level was calculated using RSEM software [36] and TPM (transcript per million) was used to normalized the ...
13
Original Article Expression profile of long noncoding RNA (HOTAIR) and its predicted target miR-17-3p in LPS-induced inflammatory injury in human articular chondrocyte C28/I2 cells
... Short-hairpin RNA directed against human lncRNA HOTAIR was ligated into the U6/GFP/ Neo plasmid (GenePharma, Shanghai, China) and was referred as to ...short-hairpin RNA directed against ETV1 was ...
12
Denaturation and Renaturation of Viral Ribonucleic Acid II. Characterization of the Products Resulting from Annealing R17 Ribonucleic Acid with Denatured Replicative Form or with Denatured Replicative Intermediate
... After digestion with ribonuclease, the double-stranded RNA found in the salt precipitate had a sedimentation profile identical to that of the doublestranded RNA found in the salt superna[r] ...
6
Single-cell analyses of transcriptional heterogeneity in squamous cell carcinoma of urinary bladder
... cell expression profile in a high throughput and low cost way offered by the early barcoding ...cell RNA- seq comprises sequential steps of target cell isolation, RNA extraction, ...
8
A robust fuzzy rule based integrative feature selection strategy for gene expression data in TCGA
... From the prediction results on the independent data (Fig. 4), the performances based on the EXP + METH were better than the EXP model in 4 of the 6 cancers, while the performances in BRCA and THCA were worse, which was ...
9