RT-Real-time PCR (RT-rPCR)
Highly Sensitive Methods Based on Seminested Real Time Reverse Transcription PCR for Quantitation of Human Immunodeficiency Virus Type 1 Unspliced and Multiply Spliced RNA and Proviral DNA
... seminested real-time PCR ...single-step real-time (RT-)PCR methods was suboptimal for the quantitative detection of HIV-1 RNA and DNA in PBMC from patients undergoing ...
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Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance
... Real-time RT-PCR is the method of choice in molecular diagnostics for detection and quantification of defined RNA molecules (Mackay ...
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Comparison of SYBR green I real time RT PCR with conventional agarose gel based RT PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco
... of PCR at 95 °C for 10 s (denaturation), 58 °C for 20 s (annealing), and 72 °C for 30 s ...post- PCR sample processing were performed in separate ...
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One step real time RT PCR assays for serotyping dengue virus in clinical samples
... A sample was classified as positive in the CDC DENV-1-4 real-time RT-PCR assays if the amplification curve crossed the threshold line within 37 cycles Five of the six DENV1 RT-PCR positi[r] ...
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Development and evaluation of a real time one step Reverse Transcriptase PCR for quantitation of Chandipura Virus
... Real-time RT-PCR has drawn significant attention in recent years as a molecular tool for detecting nucleic acids especially in virology due to rapidity, sensitivity, repro- ducibility and ...
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Establishment and Application of Digital RT PCR Assay for Detection of Avian Influenza Virus H9 Subtype
... digital RT-PCR method for rapid detection of H9 subtype influenza was es- tablished by comparing the two methods of digital RT-PCR and real-time quantitative ...
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Detection for respiratory syncytial virus rna among children with flu like illness using molecular assay
... Meanwhile, real-time RT-PCR had a higher sensitivity than viral culture for detecting RSV (Van de Pol et ...comparing RT-PCR with virus isolation and antigen detection showed ...
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Identification of Dengue Virus in Respiratory Specimens from a Patient Who Had Recently Traveled from a Region Where Dengue Virus Infection Is Endemic
... conventional RT-PCR, sequencing, and real-time RT- PCR confirmed and clarified the subtype of dengue virus that was ...conventional RT-PCR, real-time ...
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Evaluation and Validation of Real Time Reverse Transcription PCR Assay Using the LightCycler System for Detection and Quantitation of Norovirus
... load. Real-time quantitative RT-PCR systems are slowly re- placing conventional RT-PCR assays for the detection and quantification of RNA viruses in research and diagnostic lab- ...
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REAL TIME RT PCR ASSAY OF GENE FUMONISIN FUM5 RESPONSIBLE FOR MYCOTOXIN PRODUCTION ON RICE
... specific real-time reverse transcription-PCR (RT-PCR) assay to quantify the level of expression of two genes of the fumonisin biosynthetic cluster in ...
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Development and Validation of a Quantitative PCR Assay for Diagnosis of Scedosporiosis
... Two real-time PCR (RT-PCR) assays for the diagnosis of these species were developed and validated for the classification of clinical strains and for the detection of DNA in clinical ...
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External Quality Assessment for Enterovirus 71 and Coxsackievirus A16 Detection by Reverse Transcription PCR Using Armored RNA as a Virus Surrogate
... VLPs), was distributed to 54 laboratories that perform molecular diagnosis of hand, foot, and mouth disease (HFMD) virus infections. A total of 41 data sets from 41 participants were returned; 5 (12.2%) were generated ...
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Real time RT PCR high resolution melting curve analysis and multiplex RT PCR to detect and differentiate grapevine leafroll associated virus 3 variant groups I, II, III and VI
... Real-time RT-PCR amplicons of GLRaV-3 variant groups I, II, III and VI were cloned into a pGEM-T-easy Vector (Promega) and sequenced to obtain variant-specific plasmid ...DNA PCR HRM ...
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Development of one step SYBR Green real time RT PCR for quantifying bovine viral diarrhea virus type 1 and its comparison with conventional RT PCR
... nested PCR [13] for the detection of BVDV have been ...usually time-consuming and the results are not very accurate and specific ...[14]. RT-PCR is sensi- tive and specific for the detection ...
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Broadly Reactive and Highly Sensitive Assay for Norwalk Like Viruses Based on Real Time Quantitative Reverse Transcription PCR
... Nucleotide sequences of ORF1-ORF2 junction regions from 71 strains. To confirm that it is highly conserved within each genogroup, we sequenced the ORF1-ORF2 junction regions of NLVs isolated from 71 stool specimens ...
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Estrogen receptors alpha and beta in rat placenta: detection by RT-PCR, real time PCR and Western blotting
... Expression of ERα transcript in rat placentae during gestation. [A] Ethidium bromide stained gel for ERα. The migration of the 100 bp marker (M) is shown on the left- hand side and the calculated size of the signal ...
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Mechanistic Studies of Reactions at the Single-Molecule Level using Microfluidics with Applications in Molecular Diagnostics
... use RT-LAMP for HIV RNA quantification as a model system to answer the question of whether digital RT-LAMP is an attractive alternative to real-time RT-PCR for quantitative ...
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Over expression of lysophosphatidic acid receptor 2 in human invasive ductal carcinoma
... USA). Real-time PCR reactions were conducted in an ABI PRISM 7000 (Perkin-Elmer/ Applied Biosystems, Foster, CA, USA) using SYBR Green I (Perkin-Elmer) with the following profile: one step at 50°C ...
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Cost Effective Real Time Reverse Transcriptase PCR (RT PCR) To Screen for Dengue Virus followed by Rapid Single Tube Multiplex RT PCR for Serotyping of the Virus
... I-based RT-PCR and the mul- tiplex PCR differs depending on the template ...green RT- PCR displayed equal or higher sensitivity than the probe-based multiplex ...green ...
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Real Time PCR and Real Time RT PCR applications in food labelling and gene expression studies
... of real-time PCR application in food labelling and nutritional genomics, types of fluorescent- based chemistry procedures developed for real-time PCR detection, ...
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