Simian virus 40 DNA replication
Simian virus 40 DNA replication in nuclear monolayers.
... Simian virus 40 DNA replication has been studied in nuclear monolayers prepared by treatment of monolayers of BSC-1 monkey kidney cells with Noni- det P-40.. These nuclear monolayers inc[r] ...
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Role of Single-Stranded DNA Binding Activity of T Antigen in Simian Virus 40 DNA Replication
... single-stranded DNA binding domain of large T antigen to amino acid residues 259 to ...support simian virus 40 (SV40) replication and to bind to single-stranded ...Two ...
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Replication but Not Transcription of Simian Virus 40 DNA Is Dependent on Nuclear Domain 10
... simplex virus type 1, adenovirus type 5, and simian virus 40 (SV40), start their transcription and replication adjacent to a specific nuclear domain, ...viral DNA sequence ...
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Role of the Hydrophilic Channels of Simian Virus 40 T-Antigen Helicase in DNA Replication
... The simian virus 40 (SV40) hexameric helicase consists of a central channel and six hydrophilic channels located between adjacent large tier domains within each ...SV40 DNA replication, ...
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Contribution of DNA Replication to the FAM111A-Mediated Simian Virus 40 Host Range Phenotype
... of simian virus 40 (SV40) containing muta- tions in the C terminus of large T antigen fail to replicate efficiently or form plaques in restrictive cell ...sion, DNA replication, and ...
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Mutational Analysis of Simian Virus 40 T-Antigen Primosome Activities in Viral DNA Replication
... of DNA polymerase ␣ -primase (pol-prim) is a crucial step in the establishment of a functional replication complex in eukaryotic cells, but the mechanism of pol-prim loading and the composition of the ...
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Structural topography of simian virus 40 DNA replication.
... suggest an important role for the nuclear matrix in function- ally anchoring distinct large T and SV40 DNA molecules in replicational complexes, since (i) elongating SV40 DNA intermediat[r] ...
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Initiation of simian virus 40 DNA replication in vitro.
... Cosl cytoplasm containing SV40 T antigen can change the reaction of HeLa nuclear extract. from the repair to semiconservative replication[r] ...
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Simian virus 40 DNA replication: characterization of gaps in the termination region.
... Conditions for alkaline sucrose analysis of pDNA I is consistent with the absence of nicked pDNA that has been treated with T4 polymerase have been II molecules.. described above.[r] ...
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Simian Virus 40 DNA Replication in Isolated Replicating Viral Chromosomes
... The superna- tant, containing about 5 jg of viral DNA in both SV40 replicating and mature chromosomes, was referred to as a nuclear extract.. About 50% of the SV40(RI) DNA and 75% of the[r] ...
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Unwinding of Parental Strands During Simian Virus 40 DNA Replication
... Replicating SV40 molecules containing closed circular template DNA strands. Sensitivity of superhelical DNA to a single-strand specific endonuclease[r] ...
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Critical spatial requirement within the origin of simian virus 40 DNA replication.
... DNA binding studies showed that subunits of the simian virus 40 A protein (T antigen) bound to each of the four recognition pentanucleotides in the origin palindrome but did so with.. re[r] ...
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cis-active elements from mouse chromosomal DNA suppress simian virus 40 DNA replication.
... How- ever, we demonstrate that mouse DNA sequences present in the SV40 late region of the rescued clone and derived from sequences flanking the SV40 integration site in VLM DNA suppress [r] ...
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Late replicative intermediates are accumulated during simian virus 40 DNA replication in vivo and in vitro.
... These intermediates sediment in neutral sucrose as a single peak of 29S and are seen when replicative intermediates are extracted from cells and ana- lyzed directly on alkaline gradients[r] ...
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Electron microscope localization of a protein bound near the origin of simian virus 40 DNA replication.
... The SSP-DNA complex, ob- tained from either SV40-infected cells or ma- ture SV40 virus, has a novel structure when viewed by high resolution electron microscopy; the circular SV40 DNA mo[r] ...
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Wild-type p53 is not a negative regulator of simian virus 40 DNA replication in infected monkey cells.
... DNA replication in primary rhesus monkey kidney (PRK) cells, which express wild-type p53, and in the established rhesus monkey kidney cell line LLC-MK2, which expresses a mutated p53 tha[r] ...
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Large T-antigen mutants define multiple steps in the initiation of simian virus 40 DNA replication.
... The fact that C2, C6-2, and T22 displayed helicase activity is intriguing in light of the fact that these proteins failed to bind specifically to fragments containing the wild-type SV40 [r] ...
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Binding of simian virus 40 a protein to DNA with deletions at the origin of replication.
... Protein contact sites on the early and late strands are shown in Fig. 1A and B, respectively. The large brackets to the left of the figure outline DNase protection regiQns I, II, and III[r] ...
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Recently replicated simian virus 40 DNA is a preferential template for transcription and replication.
... SV40 DNA, for a subsequent round of replication, the cells were labeled for 24 h with [methyl-'4C]thymidine (0.5 "chased" in the presence of 5-bromodeoxyuridine ,Ci/ml; 40 mCi/mm[r] ...
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Effect of simian virus 40 on the temporal and spatial organization of DNA replication in Muntjac cells.
... of Muntjac cells resulted in significant differ- ences in the pooled interinitiation distance distri- bution when compared with serum-deprived cells (P = 0.0155) or with serum-enriched c[r] ...
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