Single-strand conformation polymorphism (SSCP)
Simple Method for Determining Biovar and Serovar Types of Ureaplasma urealyticum Clinical Isolates Using PCR–Single Strand Conformation Polymorphism Analysis
... procedures. Single-strand conformation polymorphism (SSCP) analysis was originally developed to detect allelic variation in human genetic disorders ...the single-stranded ...
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Capillary Electrophoresis–Single Strand Conformation Polymorphism Analysis for Rapid Identification of Pseudomonas aeruginosa and Other Gram Negative Nonfermenting Bacilli Recovered from Patients with Cystic Fibrosis
... electrophoresis–single-strand conformation polymorphism (CE-SSCP) analysis of PCR- amplified 16S rRNA gene fragments for rapid identification of Pseudomonas aeruginosa and other gram- ...
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Genotypic detection of Mycobacterium tuberculosis rifampin resistance: comparison of single strand conformation polymorphism and dideoxy fingerprinting
... or single-strand conformation polymorphism (SSCP) screening can be used to detect rpoB mutations, but these techniques either are expensive or yield results that may prove difficult to ...
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Typing of Pneumocystis carinii f sp hominis by single strand conformation polymorphism of four genomic regions
... PCR, polymorphism in each region was assessed by the single-strand conformation polymorphism (SSCP) technique, and the results for the four regions of each patient were ...a ...
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Molecular identification of bacteria by fluorescence based PCR single strand conformation polymorphism analysis of the 16S rRNA gene
... PCR–single-strand conformation polymorphism (PCR-SSCP) analysis is a rapid and convenient technique for the detection of mutations and allelic ...the single-stranded DNA is ...
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Identification of Mycobacterium Species by Multiple Fluorescence PCR–Single Strand Conformation Polymorphism Analysis of the 16S rRNA Gene
... sequent single-strand conformation polymorphism (SSCP) analysis (MF-PCR-SSCP) of four variable regions of the 16S rRNA gene were used to identify species-specific patterns for 30 ...
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Detection of rifampin resistance by single strand conformation polymorphism analysis of cerebrospinal fluid of patients with tuberculosis of the central nervous system
... by single-strand conformation polymorphism (SSCP) analysis of the amplification ...by SSCP analysis were obtained from 37 CSF specimens from 25 subjects ...
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Genetic Diversity among Borrelia Strains Determined by Single Strand Conformation Polymorphism Analysis of the ospC Gene and Its Association with Invasiveness
... within a group and ⬎8% between groups). Furthermore, Seinost et al. (17) and Baranton et al. (3) found that, among 149 sequences from data banks, 58 ospC groups can be defined within the three pathogenic species. Only 10 ...
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Application of biological and single-strand conformation polymorphism assays for characterizing potential mild isolates of Citrus tristeza virus for cross protection
... 1993), Single Strand Conformation Polymorphism (SSCP) analysis (RUBIO et ...1993). SSCP analysis is an accurate and rapid technique to identify CTV stains and its biological ...
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DETECTION SINGLE NUCLEOTIDE POLYMORPHISMS IN UROMODULIN PROMOTER REGION ASSOCIATED WITH RENAL DISEASES USING SINGLE STRAND CONFORMATION POLYMORPHISM POLYMERASE CHAIN POLYMORPHISMS TECHNIQUE
... the single-nucleotide polymorphisms (SNPs) in the UMOD gene were investigated among a big cohort of patients suffering from CKD and found that these SNPs may be implicated to the genetic standing in end- stage ...
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Rapid Identification of Bacteria from Positive Blood Cultures by Fluorescence Based PCR–Single Strand Conformation Polymorphism Analysis of the 16S rRNA Gene
... PCR–single-strand conformation polymorphism (SSCP) ...Species-specific SSCP patterns were determined for 25 of the most common bacterial species isolated from blood cultures; ...
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A single strand conformation polymorphism study of CD40 ligand Efficient mutation analysis and carrier detection for X linked hyper IgM syndrome
... by single strand conformation polymorphism (SSCP) were ...addition, SSCP analysis permitted rapid carrier detection in two families in whom the source of the mutation was most ...
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Novel Algorithm Identifies Species in a Polymycobacterial Sample by Fluorescence Capillary Electrophoresis Based Single Strand Conformation Polymorphism Analysis
... (FCE)-based single-strand conformation polymorphism (SSCP) analysis was ...resultant SSCP patterns distinguished all 19 species and ...gave SSCP patterns corresponding to ...
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Molecular analysis of patient and carrier genes with congenital steroid 21 hydroxylase deficiency by using polymerase chain reaction and single strand conformation polymorphism
... caused by point mutations of the CYP21B (CYP21A2) gene, whereas the remaining 20% are due to deletion of this gene, we used the polymerase chain reaction single strand conformation ...
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Multiple infections of Ixodes scapularis ticks by Borrelia burgdorferi as revealed by single strand conformation polymorphism analysis
... by single-strand conformation polymor- phism (SSCP) analysis, a technique which distinguishes short, fixed-length DNA fragments on the basis of nucleotide substi- ...by SSCP analysis ...
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Characterization of Salmonella Serovars by PCR Single Strand Conformation Polymorphism Analysis
... unique SSCP profile(s) (Table 2), indicating the possibility of this technique in being used for Salmonella interserovar ...three SSCP profiles were produced for the 11 serovar Enteritidis strains, and two ...
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Detection of Rifampin Resistant Mycobacterium tuberculosis in Sputa by Nested PCR Linked Single Strand Conformation Polymorphism and DNA Sequencing
... In this study, we used a nested PCR strategy targeting the 157-bp rpoB DNA, which has been most widely used for PCR- SSCP analysis and sequencing in order to detect mutations related to rifampin resistance. This ...
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Study on DGAT1-exon8 Polymorphism in Iranian Buffalo
... the polymorphism in exon 8 of the DGAT1 gene in Iranian buffaloes using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) that allows the ...
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Detection of three closely located single nucleotide polymorphisms in the EAAT2 promoter: Comparison of single strand conformational polymorphism (SSCP), pyrosequencing and Sanger sequencing
... drawbacks single-strand conformation polymorphism (SSCP) is still very frequently used across many different fields for SNP detection ...[7-15]. SSCP is a rapid, reproducible and ...
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Sequence Variations in the Bovine Growth Hormone Gene Characterized by Single-Strand Conformation Polymorphism (SSCP) Analysis and Their Association with Milk Production Traits in Holsteins
... Our objective was to screened the entire length of the bovine GH gene for sequence variations and study the associa- tions of changes in potential GH sequence variants with ch[r] ...
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