Single-stranded DNA binding proteins (SSBs)
Identification of essential and non essential single stranded DNA binding proteins in a model archaeal organism
... Single-stranded DNA-binding proteins (SSBs) play vital roles in all aspects of DNA metabolism in all three domains of life and are characterized by the presence of one or ...
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Single stranded DNA binding proteins are required for LIM complexes to induce transcriptionally active chromatin and specify spinal neuronal identities
... with single-stranded DNA-binding proteins (Ssdps or SSBPs) (Chen et ...Lhx3 proteins with NLI- homodimer lead to a tetrameric complex of 2NLI:2Lhx3, denoted as the V2-tetramer ...
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Reovirus Protein ςNS Binds in Multiple Copies to Single-Stranded RNA and Shares Properties with Single-Stranded DNA Binding Proteins
... structural proteins, NS, NS, and 1s, whose roles during viral infection remain poorly ...reovirus proteins, NS and 3, are found to associate with the viral plus-strand RNAs shortly after they are ...
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Phylogenetic and Functional Analysis of the Bacteriophage P1 Single-Stranded DNA-Binding Protein
... Single-stranded DNA-binding proteins (SSBs) have been found in all investigated organisms from bacteria to humans (7, ...These proteins have no enzymatic activity, but as ...
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Characterization of the DNA-Binding Domain of the Avian Y-Box Protein, chkYB-2, and Mutational Analysis of Its Single-Strand Binding Motif in the Rous Sarcoma Virus Enhancer
... that binding to this native double-octamer motif on the RSV LTR is comparable to the binding observed with the synthetic construct X2/E4C1, carrying a perfect repeat of the 12-mer 59-TCGTACCACCT T-39 motif ...
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Displacement of the canonical single stranded DNA binding protein in the Thermoproteales
... Single-stranded DNA binding (SSB) proteins are essential for the genome maintenance of all known cellular organisms (1) and are present in many viruses (2, ...These proteins play ...
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Developing novel single molecule analyses of the single stranded DNA binding protein from Sulfolobus solfataricus
... recruiting proteins to ssDNA who also show a slight affinity for OB ...of DNA could alter how one or many of these factors determine the binding modes of ...allows SSBs to bind strongly enough ...
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Role of Single-Stranded DNA Binding Activity of T Antigen in Simian Virus 40 DNA Replication
... tween single-stranded DNA binding and the ability to cause structure distortion of origin DNA, suggesting that single- stranded DNA binding is also needed ...
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A Single-stranded DNA-binding Protein of Bacteriophage T7 Defective in DNA Annealing
... ssDNA binding activity and pairing of gene ...protein-bound DNA strands. It is clear that ssDNA binding is required, since gene ...2.5 proteins that do not bind DNA are also defective ...
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Mutations in Sensor 1 and Walker B in the Bovine Papillomavirus E1 Initiator Protein Mimic the Nucleotide-Bound State
... groups—E1 proteins, T antigens, and Rep proteins—have pro- vided important information about how ATP is bound and hydrolyzed by these proteins and the structural consequences that result (1, 4, 7, ...
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Dominant mutations in mtDNA maintenance gene SSBP1 cause optic atrophy and foveopathy
... Identification of mutations in SSBP1 in families with dominant optic atrophy. We performed whole exome sequencing (WES) in one French family (family A) including multiple patients with ADOA to identify the causative gene ...
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Evidence for DNA Hairpin Recognition by Zta at the Epstein-Barr Virus Origin of Lytic Replication
... hairpin binding are also replication ...in DNA replication but no apparent defects in binding to double- stranded ZRE ...Zta proteins with single amino acid changes within the ...
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Analyses of the Interaction between the Origin Binding Domain from Simian Virus 40 T Antigen and Single-Stranded DNA Provide Insights into DNA Unwinding and Initiation of DNA Replication
... for DNA replication; the references to the individual mutations are ...in binding to double-stranded DNA substrates ...for binding to single-stranded DNA, ...
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The 2B subdomain of Rep helicase links translocation along DNA with protein displacement
... catalyse DNA and RNA strand separation. Proteins bound to the nucleic acid must also be displaced in order to unwind ...from DNA ahead of advancing replication forks. How helicases catalyse ...
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Single molecule FRET analysis of DNA binding proteins
... a single fluorophore reveal reaction intermediates dur- ing transcription ...of single UvrD helicase dynamics on long single-stranded ...on single-stranded DNA to ...
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The 60-Residue C-Terminal Region of the Single-Stranded DNA Binding Protein of Herpes Simplex Virus Type 1 Is Required for Cooperative DNA Binding
... a binding pattern characteristic of multiple independent sites, in striking contrast with the highly cooper- ative behavior of the full-length ICP8 and ...the binding of the C-terminally truncated ...
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The helicase XPD unwinds bubble structures and is not stalled by DNA lesions removed by the nucleotide excision repair pathway
... of DNA helicases with iron–sulphur cluster ...in DNA unwinding during nucleotide excision repair ...a DNA bubble structure, differentiating it from the related helicases FancJ and ...that DNA ...
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Identification of a Region of the Herpes Simplex Virus Single-Stranded DNA-Binding Protein Involved in Cooperative Binding
... cooperative binding of ICP8 may be important in its helix- destabilizing activity (2, ...cooperative binding of ICP8 is affected by FM modification, helix-destabilizing experiments comparing ICP8 and ...
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Schüller, Verena (2013): DNA origami structures for applications in single molecule spectroscopy and nanomedicine. Dissertation, LMU München: Fakultät für Physik
... a DNA origami- based design for the simplest architecture of this kind; a four- faced tetrahedron consisting of six flexibly jointed ...the DNA origami technique, its triangular base was used as a stage for ...
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Genome sequence and description of Timonella senegalensis gen. nov., sp. nov., a new member of the suborder Micrococcinae
... P167 = DSMZ 25696) was grown aerobically on 5% sheep blood-enriched BHI agar at 37°C. The growth from four Petri dishes was collected and resuspended in 4×500 µl of TE buffer and stored at -20°C. Then, 500 µl of this ...
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