TK, thymidine kinase
In vitro Interaction of HSV-1 ORF P with Both Thymidine Kinase (TK) and an Unidentified Cellular Protein
... As most ICP34.5 null mutants affect ORF P due to the extensive overlap of their sequences, it is difficult to assign a role to ORF P [6-9]. An approach to analyze the function of a viral gene such as ORF P is to ...
5
Capture of DNA Sequences at Double-Strand Breaks in Mammalian Chromosomes
... transfected thymidine kinase (tk)-deficient mouse fibroblasts with a DNA substrate containing a recognition site for yeast endonuclease I-SceI embedded within a functional tk ...lost tk ...
10
High-Capacity Adenovirus Vector-Mediated Anti-Glioma Gene Therapy in the Presence of Systemic Antiadenovirus Immunity
... Glioblastoma multiforme (GBM) is a malignant primary brain tumor associated with ⬍ 2% survival at 5 years postdiag- nosis. Advances in neurosurgery, chemotherapy, and radio- therapy have not impacted the dire clinical ...
6
The relationship between DNA double strand breaks and mutation induction following treatment with X rays and restriction endonucleases
... the tk locus (encoding for the enzyme thymidine kinase) following treat ment of Chinese hamster ovary cells (CHO KI) with two restriction endonucleases (REs), FvuH and EcoRI, which generate ...
203
In Vitro-Selected Drug-Resistant Varicella-Zoster Virus Mutants in the Thymidine Kinase and DNA Polymerase Genes Yield Novel Phenotype-Genotype Associations and Highlight Differences between Antiherpesvirus Drugs
... VZV TK than in HSV-1 and HSV-2 TKs (44). It appears that VZV TK is rela- tively conserved, and only a very low number of genetic polymor- phisms have been described ...VZV TK always occurred in com- ...
12
The herpes simplex virus type 1 (HSV-1) a sequence serves as a cleavage/packaging signal but does not drive recombinational genome isomerization when it is inserted into the HSV-2 genome.
... Thus, HSV recombinants bearing an additional copy of the a sequence inserted into the thymidine kinase tk gene display all of the additional genome isomers that are predicted to arise fr[r] ...
6
Rapid Phenotypic Characterization Method for Herpes Simplex Virus and Varicella Zoster Virus Thymidine Kinases To Screen for Acyclovir Resistant Viral Infection
... (VZV) thymidine kinase (TK) genes was developed for monitoring acyclovir-resistant ...the TK polypeptide, which is synthesized in vitro from viral DNA using a procedure as ...The TK ...
6
Functions of Fission Yeast Orp2 in DNA Replication and Checkpoint Control
... selection for leucine prototrophy; transformants were sensitive used here should be widely useful in fission yeast stud- to 25 mm FUdR (Sigma, St. Louis, cat. F-0503) indicating ies. The approach is also applicable to ...
9
Temperature-Dependent Conformational Changes in Herpes Simplex Virus ICP4 That Affect Transcription Activation
... the thymidine kinase gene (tk) promoter, the mutant plas- mids or wt pK1-2 was cotransfected together with plasmid pLSWT into mono- layers of Vero cells as described ...of tk from HSV-1 ...
12
Isolation of cis-acting vaccinia virus DNA fragments promoting the expression of herpes simplex virus thymidine kinase by recombinant viruses.
... TK- cells were infected with one of these recombinant viruses and then transfected with pools of chimeric plasmids composed of a cloned herpes simplex virus thymidine kinase gene which c[r] ...
10
Insertion mutants of herpes simplex virus have a duplication of the glycoprotein D gene and express two different forms of glycoprotein D.
... Insertion of appropriate HSV-1 DNA fragments into the thymidine kinase TK gene of a gC- HSV-1 mutant permitted us to monitor expression of gC from the inserted DNA sequences of selected [r] ...
9
Development of a helper-independent human adenovirus vector and its use in the transfer of the herpes simplex virus thymidine kinase gene.
... To test the usefulness of this vector, the herpes simplex virus type 1 HSV-1 thymidine kinase gene tk along with its regulatory sequences was inserted into the unique XbaI site of dlEl,3[r] ...
8
Improved retroviral suicide gene transfer in colon cancer cell lines after cell synchronization with methotrexate
... Background: Cancer gene therapy by retroviral vectors is mainly limited by the level of transduction. Retroviral gene transfer requires target cell division. Cell synchronization, obtained by drugs inducing a reversible ...
12
Mutational analysis of the ICP4 binding sites in the 5' transcribed noncoding domains of the herpes simplex virus 1 UL 49.5 gamma 2 gene.
... USA 86:4071-4075, 1989 demonstrated that substitution of sequences of the thymidine kinase tk gene, a 13 gene, extending from -16 to +51 with sequences extending from -12 to +104 of the [r] ...
9
Somatostatin receptor type 2 as a radiotheranostic PET reporter gene for oncologic interventions
... 1 thymidine kinase; LV: lentiviral vector; MRI: magnetic resonance imaging; mMSC: murine mesenchymal stem cell; MSC: mesenchymal stem cell; NECA: neuroendocrine carcinoma; NIS: sodium iodine symporter; PCR: ...
12
Splicing-Independent Expression of the Herpes Simplex Virus Type 1 Thymidine Kinase Gene Is Mediated by Three cis-Acting RNA Subelements
... binds TK mRNA and facilitates its expression through an NES- dependent pathway ...in TK mRNA pathway usage are most likely due to in vivo conditions that vary between HSV infections and the transient ...
8
Expression and characterization of the thymidine kinase gene of African swine fever virus.
... 1046 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The thymidine kinase TK gene of African swine fever virus ASFV was located within the viral genome by using two deg[r] ...
7
Substitution of a TATA box from a herpes simplex virus late gene in the viral thymidine kinase promoter alters ICP4 inducibility but not temporal expression.
... A set of recombinant viruses was constructed such that expression of an HSV-1 early gene, thymidine kinase tk, was placed under the control of either the tk TATA box or the TATA box from[r] ...
11
Evidence for a novel regulatory pathway for herpes simplex virus gene expression in trigeminal ganglion neurons.
... 5383 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Thymidine kinase TK-negative TK- mutant strains of herpes simplex virus type 1 HSV-1 show reduced expression of a an[r] ...
11
Structural features of the herpes simplex virus alpha gene 4, 0, and 27 promoter-regulatory sequences which confer alpha regulation on chimeric thymidine kinase genes.
... Chimeric genes constructed by fusion of the coding and 5' nontranslated leader sequences of the thymidine kinase TK gene to the sequences upstream from the site of initiation of transcri[r] ...
11