[PDF] Top 20 The allocation and differentiation of mouse primordial germ cells

... epiblast cells of the early-streak stage mouse embryo reveals that the developmental fate of the cells is determined by their position in the germ ...Epiblast cells that are fated to ... See full document

... ABSTRACT Mouse primordial germ cells (PGCs) migrate from the base of the allantois to the genital ...sex- differentiation of fetal ...of mouse PGCs has been studied by culturing ... See full document

... ES cells (Susanto et al., 2008). Al- though PGCs are germ line-committed cells under certain in vivo and in vitro conditions, they can actually transform into embryonal carcinoma (EC) and embryonal ... See full document

... epiblast cells, PGCs and somatic ...the mouse genome ...– Mouse Genome Informatics), respectively (supplementary material ...epiblast cells, which are a source of both PGCs and somatic ... See full document

... In addition to the causes of apoptosis discussed above, recent evidence indicate that PGCs are subjected to another source of cell death, abnormal genome reprogramming. In the last few years, some studies reported that ... See full document

... male germ cell can be successfully induced from iPS/ES cells in vitro and fully differentiated into spermatocytes in vivo ...male germ cells, the differentiation of human oocytes from ... See full document

... somatic cells To further examine possible off-target effects in vivo, we analyzed peri-implantation development of ER-Cre/NRi-Tg double- transgenic embryos generated by mating females homozygous for NRi-Tg with ... See full document

... blood cells; mbc, maternal blood cells; stc, spongiotrophoblast; la, labyrinthine; tgc, trophoblast giant cells; ys, yolk sac; al, allantois; cp, chorionic plate; am, amnion; da, dorsal aorta; 1, ... See full document

... oocyte-like cells were surrounded by one to two layers of flattened cells and did not have a visible zona pellucida; oocyte-specific markers, such as Fig and Zp3, were expressed by the ovarian ... See full document

... and Donner, 2001; Zhou et al., 2001). As shown in Fig. 5B, only a faint MDM2 signal was detected in the cytoplasm of transgenic PGCs in the absence of 4OHT. However, the percentage of PGCs that showed stronger MDM2 ... See full document

... PGC differentiation is a rather passive process in mice, such that Rhox6 may not play a role in autonomously directing the fate of ESCs to form ...differentiated cells after electroporation of ESCs with ... See full document

... stem cells and thereby facilitate its recruitment to PRDM14 target genes [19, 31] or that PRDM14 could activate expression of the PRC2 com- ponent Suz12 ...like cells cultured in vitro lose DNA-methylation, ... See full document

... PGC-like cells derived from human embryonic stem cells maintain NANOG expression, express very low or no PRDM14, and do not express SOX2 (Irie et ...of mouse and humans, and it is not yet clear ... See full document

... postmigratory primordial germ cells coincides with erasure of genomic imprints and reactivation of the inactive X chromosome, as well as ongoing germ cell differentiation ...in ... See full document

... In adult neurons, activity induced GADD45B has been shown to demethylate DNA actively at promoters of key genes involved in adult neurogenesis and to induce their expression (Ma et al., 2009). Furthermore, another study ... See full document

... All experiments involving animals in the study were conducted according to the Care and Use of Experi- mental Animals Guidelines of the Canadian Council on Animal Care, and have been approved by the Western University ... See full document

... adhering cells and lose their locomotory and proliferation ...somatic cells and enter the long process of differen- tiation into the gametes of the ... See full document

... somatic cells, cell suspensions were plated into culture flasks and maintained at 32 ° C overnight in DMEM supplemented with 10% FCS, 32 mM NaHCO3, 2 mM glutamine, 100 U/ml penicillin and 100 µ g/ml streptomycin ... See full document

... Mammalian germ cells undergo unique genetic and cellular changes as they develop and differentiate to form functional ...epiblast cells at around 6.5 days post coitum (dpc) gives rise to ... See full document

... homogeneous cells and antisera with high ...fewer cells than ChIP-based ...in mouse embryonic stem cells, epiblast-like cells and primordial germ cell-like cells ... See full document