[PDF] Top 20 Amphipathic Helix-Dependent Localization of NS5A Mediates Hepatitis C Virus RNA Replication
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Amphipathic Helix-Dependent Localization of NS5A Mediates Hepatitis C Virus RNA Replication
... of NS5A was ...of NS5A abolishes the intracellular membrane localization pattern of ...of NS5A found to harbor an AH is shown in gray (above the picture) and in more detail in the bottom ... See full document
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Amphipathic α-Helix AH2 Is a Major Determinant for the Oligomerization of Hepatitis C Virus Nonstructural Protein 4B
... interactions. Amphipathic ␣ -helix AH2 was identified as a major determinant for the oligomerization of ...HCV RNA replication, implying that oligomerization of NS4B is essential for at least ... See full document
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All Three Domains of the Hepatitis C Virus Nonstructural NS5A Protein Contribute to RNA Binding
... UTR RNA. NS5A is an essential component of the HCV replication com- plex and has been shown to bind RNA; however, the domains of NS5A that are responsible for RNA binding in ... See full document
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A Point Mutation in the N-Terminal Amphipathic Helix α0 in NS3 Promotes Hepatitis C Virus Assembly by Altering Core Localization to the Endoplasmic Reticulum and Facilitating Virus Budding
... the localization of core proteins from LD to the ER. The nascent HCV RNA genomes are encapsidated by core proteins on lipid droplets (LD), and the resulting nucleocapsids are then translocated to the ER for ... See full document
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Intragenic Complementation of Hepatitis C Virus NS5A RNA Replication-Defective Alleles
... of replication-defective NS5A ...three NS5A complementation groups (groups A, B, and C [Table 1]), each of which is potentially associated with a distinct RNA replication ... See full document
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Identification of Residues Required for RNA Replication in Domains II and III of the Hepatitis C Virus NS5A Protein
... in RNA replication relative to the parent, suggesting that this mutation may confer a weak ...the hepatitis C virus NS5A protein. NS5A has been proposed to consist of ... See full document
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Visualization of Double-Stranded RNA in Cells Supporting Hepatitis C Virus RNA Replication
... HCV RNA synthesis, in which genome replication occurs within ER-derived vesicles of the membranous web whose formation is believed to be driven, at least in part, by the oligomerization of NS4B (10, 15, ... See full document
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Binding Dynamics of Hepatitis C Virus' NS5A Amphipathic Peptide to Cell and Model Membranes
... the hepatitis C virus NS5A protein is required for viral ...is dependent on an N-terminal amphipathic helix (AH) within NS5A and is restricted to a subset of host ... See full document
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An Amphipathic α-Helix at the C Terminus of Hepatitis C Virus Nonstructural Protein 4B Mediates Membrane Association
... viral replication complexes ...and NS5A colocalized in cytoplasmic dot-like ...and NS5A to lipid droplets, which are abundant in Huh-7 cells, rendered the identification of dot-like structures as a ... See full document
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Cyclophilin A Interacts with Domain II of Hepatitis C Virus NS5A and Stimulates RNA Binding in an Isomerase-Dependent Manner
... of NS5A, derived from both the genotype 1b J4 isolate and the geno- type 2a JFH-1 isolate ...the virus-positive strand genomic RNA, although the binding of domain III was weak in comparison to that ... See full document
5
A Rab-GAP TBC Domain Protein Binds Hepatitis C Virus NS5A and Mediates Viral Replication
... HCV NS5A and TBC1D20 is sup- ported by several independent assays, including functional in- teraction within live cells ...between NS5A and TBC1D20 can be demonstrated in multiple independent systems, ... See full document
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Calcium-Dependent Calpain Proteases Are Implicated in Processing of the Hepatitis C Virus NS5A Protein
... the NS5A protein remains an unsolved puzzle, inasmuch as our data indicate that NS5A cleavage favors the generation of C- terminally truncated NS5A forms which lack these ...study, NS5A ... See full document
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Interaction between Nonstructural Proteins NS4B and NS5A Is Essential for Proper NS5A Localization and Hepatitis C Virus RNA Replication
... the NS5A anchor charged ...HCV RNA replication were included for rever- sion analysis. RNA from each mutant was electroporated into ...HCV RNA that restored replicative fitness. As no ... See full document
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An N-Terminal Amphipathic Helix in Hepatitis C Virus (HCV) NS4B Mediates Membrane Association, Correct Localization of Replication Complex Proteins, and HCV RNA Replication
... luminal localization after the integration of the protein’s TMDs into the membrane ...N-terminal amphipathic helix in NS4B mediates ... See full document
8
Membrane Association of the RNA-Dependent RNA Polymerase Is Essential for Hepatitis C Virus RNA Replication
... and RNA replication of the LVL mu- ...subcellular localization and membrane association that were indistinguishable from those of wild-type ...its RNA replication was com- pletely ... See full document
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RNA Replication without RNA-Dependent RNA Polymerase: Surprises from Hepatitis Delta Virus
... and helix- loop-helix domains and a stretch of proline- and glycine-rich sequence at the C ...DNA-dependent RNA polymerase II (pol ...HDV RNA replication, it tends to form ... See full document
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A Nucleotide Binding Motif in Hepatitis C Virus (HCV) NS4B Mediates HCV RNA Replication
... As shown in Fig. 2A, NS4B-GFP, but not GFP, was specif- ically labeled with GTP (Fig. 2A, lane 1 versus 2). Western analysis with an antibody against GFP of the immunoprecipi- tates revealed comparable expression levels ... See full document
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3′ Nontranslated RNA Signals Required for Replication of Hepatitis C Virus RNA
... transient replication assays carried out with the indicated ...Ntat2ANeo(SI) RNA, respectively; ⫺, SEAP activity indistinguishable from that of the ⌬GDD mutant RNA; (⫹), pseudolethal mutation that ... See full document
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Human VAP-B Is Involved in Hepatitis C Virus Replication through Interaction with NS5A and NS5B
... HCV RNA replication (19, 49, ...interfering RNA (siRNA) or the domi- nant-negative mutant of VAP-A resulted in relocation of NS5B from detergent-resistant to detergent-sensitive mem- branes and ... See full document
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Serine phosphorylation of the hepatitis C virus NS5A protein controls the establishment of replication complexes
... SDS-PAGE/Western blotting. Cells were washed twice in PBS, lysed in 1⫻ Glasgow lysis buffer (GLB; 1% [vol/vol] Triton X-100, 120 mM KCl, 30 mM NaCl, 5 mM MgCl 2 , 10% [vol/vol] glycerol, 10 mM PIPES [piperazine-N,N = ... See full document
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