[PDF] Top 20 Architecture of Replication Compartments Formed during Epstein-Barr Virus Lytic Replication

... replication compartments. The numbers of the spots representing replication compartments at early stages of the lytic replication were more than 10, which is lower than the ... See full document

... viral replication compartments, SRp20 localized to nodules at the surface of viral replication ...VINORCs during lytic EBV ...the lytic phase of EBV infection by treatment with ... See full document

... induce lytic replication and harvested at the indicated postinduction ...(B) Lytic replication-induced cells were stained with anti-BMRF1 (green) and an- ti-RNAPII ... See full document

... DNA during lytic ...synthesized during lytic replication ...viral replication compartments ...from lytic rep- lication-induced Tet-BZLF1/B95-8 cells according to ... See full document

... Zta, like UL9, is able to bind specifically to a single-stranded imperfect DNA hairpin formed within the top strand of a critical element within the lytic origin (Fig. 1). In EBV, this cis-acting component ... See full document

... inhibits lytic replication of ...KSHV lytic replication ...EBV formed type II latency with low levels of LMP-1 expression in these EBV convertants used in present study, which is in ... See full document

... replication stages, as represented by the middle cell, PML is still concentrated in several ND10-like ...no lytic activation was induced. The replication-specific probe fails to recognize ... See full document

... 25). Lytic replication was induced by treatment with 20 ng of tetradecanoyl phorbol acetate (TPA) per ml for 3 ...intact during antibody treatment procedure, preventing antibody access to ... See full document

... and replication assays performed in EBV-positive P3HR1 cells, the Zta (m22/26,74/75) mutant was replication ...cells, replication compartments could be detected by im- munofluorescence ... See full document

... affinity gel (Sigma). The agarose matrix was washed extensively, and SM complexes were eluted with 150 ␮ l of 1-mg/ml FLAG peptide in Tris-buffered saline, with shaking for 30 min at room temperature. Vector-transfected ... See full document

... the replication compartments (32, 56, ...ATM during lytic reactivation of EBV in infected nasopharyngeal carcinoma cells, we also dem- onstrated active participation of ATM activation in the ... See full document

... oriLyt replication in a manner independent of its transcriptional ac- tivator ...oriLyt replication, although transcription can be restored ...oriLyt-mediated replication (21). Although BZLF1 is ... See full document

... HSV1 lytic rep- ...EBV replication, we tested the effect of the enzyme on replication of the alphaherpesvirus ...is formed at the G-rich BHLF1 region during initiation of lytic ... See full document

... are formed or reorganized by viral and cellular ...these compartments are diverse but, invariably, include components that direct viral genome replica- tion and ...of compartments where viral ge- ... See full document

... ABSTRACT Epstein-Barr virus (EBV) is linked to the development of both lymphoid and epithelial malignancies ...spontaneous lytic replication and high-titer virus production in ... See full document

... EBV replication suggested that LF2 may be an immediate-early gene and could activate its own promoter but not EBV replication (4, ...the replication-competent B95-8 strain lacks this ...a ... See full document

... In addition to the two induction systems used to study transcription of EBERs and EBNA mRNAs, we also looked at EBNA abundance in Raji cells which had been induced to make early antigens[r] ... See full document

... mutant-transforming virus and would have been provided by wild-type B95-8 virus present in the trans- fected B95-8 cells in which the recombinant was ...issue, virus replication was induced in ... See full document

... * 1786 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The Epstein-Barr virus nuclear antigen 1 EBNA1 protein binds and activates the latent replication origin oriP of [r] ... See full document

... Deletions that left six or fewer 30-bp repeats in an EBV plasmid or plasmids reconstructed with up to five copies of a 30-bp repeat oligonucleotide were defective for plasmid maintenance[r] ... See full document