[PDF] Top 20 Binding of tRNA to Reverse Transcriptase of RNA Tumor Viruses
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Binding of tRNA to Reverse Transcriptase of RNA Tumor Viruses
... The binding of tRNA and avian sarcoma virus 6, and proline tRNA changes the molecular weight of the mammalian has recently been shown to be the primer in enzyme from 70,000 to 150,000, s[r] ... See full document
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Mutations in the U5 Region Adjacent to the Primer Binding Site Affect tRNA Cleavage by Human Immunodeficiency Virus Type 1 Reverse Transcriptase In Vivo
... vectors. Viruses were generated by cotrans- fecting 293 cells with the HIV-1 vectors and pHMCV-g, a plasmid that expresses the VSV glycoprotein (3, ...murine RNA Pol II promoter; EM-Zeo, EM-7 ... See full document
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Binding of tryptophanyl-tRNA to the reverse transcriptase of replication-defective avian sarcoma viruses.
... The ability of the into virions is a temperature-sensitive process, mutant viruses to absorb the monospecific IgG and virions produced at 420C contain less re- can then be estimated by a[r] ... See full document
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Telomerase reverse transcriptase promotes cancer cell proliferation by augmenting tRNA expression
... Genome-wide binding of endogenous TERT reveals its association with RNA polymerase III target ...with RNA poly- merase I (pol I) under hyperproliferative conditions and thus stim- ulates ribosomal ... See full document
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Chaperones Activate Hepadnavirus Reverse Transcriptase by Transiently Exposing a C-Proximal Region in the Terminal Protein Domain That Contributes to ε RNA Binding
... B viruses (HBVs), or hepadnaviruses, are small enveloped DNA-containing viruses (12, 34) that replicate through protein-primed reverse transcription of a pregenomic RNA (pgRNA) intermediate ... See full document
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Sequence- and structure-specific determinants in the interaction between the RNA encapsidation signal and reverse transcriptase of avian hepatitis B viruses.
... otides as in the K variants or a single substitution as in variant L4, led to reduction if not complete loss of function. With the following experiments, we sought to link these functional dif- ferences with the ... See full document
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Reverse Transcriptase- and RNA Packaging Signal-Dependent Incorporation of APOBEC3G into Hepatitis B Virus Nucleocapsids
... cellular RNA (30, 37, 54, 57, 58, ...ε RNA packaging ...an RNA-independent manner, which suggests that A3G is incorporated into the HBV nucleocapsid via its inter- action with ...the binding ... See full document
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Reverse transcriptase of RNA tumor viruses. V. In vitro proteolysis of reverse transcriptase from avian myeloblastosis virus and isolation of a polypeptide manifesting only RNase H activity.
... 25, 1978 PROTEOLYSIS OF AMV REVERSE TRANSCRIPTASE sequences only present in subunit ,B; and iii a smaller polypeptide of molecular weight 24,000 was isolated, which contained only RNase [r] ... See full document
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Studies on reverse transcriptase of RNA tumor viruses. I. Localization of thermolabile DNA polymerase and RNase H activities on one polypeptide.
... The three known enzymatic activities associated with the DNA polymerase of avian RNA tumor viruses were compared using polyC oligodG to measure RNA-directed DNA synthesis, polydC oligodG[r] ... See full document
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Studies on reverse transcriptase of RNA tumor viruses III. Properties of purified Moloney murine leukemia virus DNA polymerase and associated RNase H.
... When [3H]polyA -polydT was used as a substrate, the size of the M-MuLV DNA polymerase-associated RNase H digested product was larger than the size of the digestion products by AMV DNA po[r] ... See full document
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Development of Real Time Reverse Transcriptase PCR Assays To Detect and Serotype Dengue Viruses
... viral RNA consisting of eight mosquito-borne flaviviruses in the FP and 109 DENV strains in the ...using RNA extracted from FP specimens and utilizing the conventional heminested and SYBR green pro- ... See full document
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Hepatitis B Virus Reverse Transcriptase and ε RNA Sequences Required for Specific Interaction In Vitro
... RT binding with the sequence-specific recogni- tion of the DHBV ε loop by its cognate DHBV RT (6, ...RT binding, base pairing in the corresponding ε region in the avian viruses is not required for RT ... See full document
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The connection domain in reverse transcriptase facilitates the in vivo annealing of tRNALys3 to HIV 1 genomic RNA
... the viruses produced from the different transfections, probed with anti-CA, and shows that all forms of GagPol deletion mutants tested here are incorporated into the ...viral RNA was isolated from these ... See full document
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Mutations Proximal to the Minor Groove-Binding Track of Human Immunodeficiency Virus Type 1 Reverse Transcriptase Differentially Affect Utilization of RNA versus DNA as Template
... dNTP binding and not due to a defect in a primary event such as initial recognition and binding of ...on RNA templates in that the polymerization stopped after the addition of a single ...both ... See full document
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RNA-Dependent DNA Polymerase Activity of RNA Tumor Viruses II. Directing Influence of RNA in the Reaction
... The polymerase catalyzes the synthesis of DNA in the presence of four deoxynucleoside triphosphates, Mg2+, and a variety of RNA templates including those isolated from avian myeloblastos[r] ... See full document
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AZT resistance alters enzymatic properties and creates an ATP binding site in SFVmac reverse transcriptase
... nucleotide binding affinity and increased ...ATP binding could be detected in the corresponding wild type ...ATP binding, probably by making a tryptophan residue ... See full document
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A defective retrovirus particle (SE21Q1b) packages and reverse transcribes cellular RNA, utilizing tRNA-like primers.
... In all cases the reaction mixtures contained [32P]dTTP either alone lane 1 or with dATP lane 2; dCTP lane 3; dGTP lane 4; or with dATP, The Rous sarcoma virus RSV-transformed quail cell [r] ... See full document
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Immunological Relationships of Reverse Transcriptases from Ribonucleic Acid Tumor Viruses
... Since antisera to the R-MuLV reverse transcriptase were equally potent whether tested against disrupted whole virus or partially purified enzyme, lack of inhibition of the reverse transc[r] ... See full document
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Comparative Study of Various Non-Nucleoside Reverse Transcriptase Inhibitors on Different Reverse Transcriptase Enzyme
... In the present work a library of synthesized thiazolidi- none based New chemical entities (NCE’s) with anti HIV activity were used for pharmacophore optimization by studying their binding behavior with four ... See full document
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Quantitation of RNA tumor viruses by spectroscopy of density gradient gels.
... Using 0.8-ml gradients gels 4 by 45 mm we can detect as low as 2 x 107 viral particles with Coomassie blue staining and 6 x 106 viral particles with a more sensitive staining procedure u[r] ... See full document
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