[PDF] Top 20 Cell and viral regulatory elements enhance the expression and function of a human immunodeficiency virus inhibitory gene.
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Cell and viral regulatory elements enhance the expression and function of a human immunodeficiency virus inhibitory gene.
... lineage-specific expression of a CAT reporter with regu- latory elements from the murine CD4 gene was reported pre- viously ...339-bp regulatory fragment from a region ; 13 kb upstream of the ... See full document
10
cis-Acting inhibitory elements within the pol-env region of human T-cell leukemia virus type 1 possibly involved in viral persistence.
... of human immunodeficiency virus (HIV) have been shown to reduce the expression of chimeric reporter genes (5, 10, 35, ...The inhibitory effects of such sequences mapped to gag, pol, and ... See full document
10
Negative and Positive mRNA Splicing Elements Act Competitively To Regulate Human Immunodeficiency Virus Type 1 Vif Gene Expression
... cis elements that contributed to the regulation of splicing at 3 ⬘ ss ...and cell supernatants were harvested. RT-PCR was used to detect viral RNA species in the mutant ⬃ ...and expression of ... See full document
11
An autoregulated dual-function antitat gene for human immunodeficiency virus type 1 gene therapy.
... vivo gene therapy against HIV-1 ...the inhibitory gene into PBMCs or purified CD4 1 lymphocytes isolated from HIV-1-infected individuals and to select and reinfuse these cells into the patient ...the ... See full document
7
Contributions of Viral Splice Sites andcis-Regulatory Elements to Lentivirus Vector Function
... leukemia virus-derived vectors in transient and long-term transduction of various types of human cells (11, 23, 30, ...essential viral sequences without compromising vector func- tions. The ... See full document
6
Cellular Gene Expression upon Human Immunodeficiency Virus Type 1 Infection of CD4+-T-Cell Lines
... single-spliced viral RNA messages needed at the later stages of the virus life ...the expression of seven DEAD- box proteins (DDX, RNA helicases), several elongation initi- ation factors, and ... See full document
11
CDK13, a New Potential Human Immunodeficiency Virus Type 1 Inhibitory Factor Regulating Viral mRNA Splicing
... to enhance the function of Tat in transcriptional elongation (17, ...novel function, that of viral mRNA splicing regulation ...full virus, we cloned the pNL4-3 (K50, 51A) double mutant ... See full document
12
Role of Regulatory Elements and the MAPK/ERK or p38 MAPK Pathways for Activation of Human Cytomegalovirus Gene Expression
... recombinant virus RVUL4CATgpt, which has a 2,115-bp BsrGI-BamHI DNA fragment con- taining the guanine phosphoribosyltransferase gene (gpt) under the control of a minimal simian virus 40 (SV40) ... See full document
13
Recognition of prominent viral epitopes induced by immunization with human immunodeficiency virus type 1 regulatory genes.
... three regulatory proteins since the in vitro T-cell immune responses were stron- ...protein expression and T-cell presentation probably occur in the Langerhans cells in the skin or in closely ... See full document
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Inactivation of the human immunodeficiency virus type 1 inhibitory elements allows Rev-independent expression of Gag and Gag/protease and particle formation.
... down-regulatory elements raises the question of their mode of ...these elements were low- ered by about ...the viral INS elements contain the AUUUA pentanucleotide, which has been shown ... See full document
12
The nef gene products of both simian and human immunodeficiency viruses enhance virus infectivity and are functionally interchangeable.
... of function between HIV-1 and SIV Nef in tissue culture infections suggests that they may also exert analogous effects in vivo, with HIV-1 Nef, like SIV Nef, acting to facilitate high level virus ...to ... See full document
11
Methylation of Tat by PRMT6 Regulates Human Immunodeficiency Virus Type 1 Gene Expression
... Tat function and led to the production of higher levels of viral ...negative regulatory mecha- nisms within cells in order to achieve optimal viral gene ex- ...of gene ... See full document
8
Evidence for Gene Expression by Unintegrated Human Immunodeficiency Virus Type 1 DNA Species
... the viral promoter in direct orientation, in contrast to the other viral DNA ...region, viral transcripts initiated from the upstream start site would contain the U5-U3 sequence ...quantify ... See full document
9
Human immunodeficiency virus type 1 integrase: effects of mutations on viral ability to integrate, direct viral gene expression from unintegrated viral DNA templates, and sustain viral propagation in primary cells.
... integrase gene were con- structed (Table ...mutant virus stocks regardless of the integrity of the integrase function, the proviral constructs were transfected into a COS-7 cell line and ... See full document
11
Novel Function of Prothymosin Alpha as a Potent Inhibitor of Human Immunodeficiency Virus Type 1 Gene Expression in Primary Macrophages
... control human immunodeficiency virus type 1 (HIV-1) infection by a cytotoxic major histocompatibility complex-restricted pathway as well as by secretion of noncytotoxic soluble inhibitory ...ⴙ ... See full document
7
The effect of viral regulatory protein expression on gene delivery by human immunodeficiency virus type 1 vectors produced in stable packaging cell lines.
... The deletion of pCMVrev from the transfection of 5BD.1 cells did not have any effect on the titer of the vector produced from these cells. However, in the case of 2A.22 cells, deletion of pCMVrev reduced the vector titer ... See full document
8
Primary Sooty Mangabey Simian Immunodeficiency Virus and Human Immunodeficiency Virus Type 2 nef Alleles Modulate Cell Surface Expression of Various Human Receptors and Enhance Viral Infectivity and Replication
... p239MluI (5⬘-GTCCCTACGCGTCAGCGAGTTTCCTTCTTG-3⬘) (mutated positions are lowercase; underlining represents the XbaI and MluI restriction sites), resulting in an M1V change and introducing two premature stop codons at ... See full document
14
Cellular Mobile Genetic Elements in the Regulatory Region of the Pneumotropic Mouse Polyomavirus Genome: Structure and Function in Viral Gene Expression and DNA Replication
... of viral DNA synthesis and in tran- scription of the late region have been demonstrated ...the virus can ...the virus the ability to multiply in mouse fibroblast cells ...Corresponding ... See full document
10
Viral and host gene expression in human B-cell lymphoma
... and viral DNA replication (see section ...the virus life cycle and so provides information on both the function of virus genes and the mechanisms of virus ...by expression. This ... See full document
327
Expression of a Sheep Pox Virus Gene in Plant Systems under the Control of Plant Viral Regulatory Elements and with Sub-Cellular Targeting
... germ cell free system for 1 h at 26 °C, and then the recombinant A27L protein was analyzed by SDS-PAGE and subsequent western blotting ...not enhance translation 19 ... See full document
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