[PDF] Top 20 Complementation Analysis of Simian Virus 40 Mutants
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Complementation Analysis of Simian Virus 40 Mutants
... The pattern of complementation amongst late mutants, those capable of viral DNA replication at the nonpermissive temperature unpublished results, appears to be somewhat more complex than[r] ... See full document
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Genetic and biochemical analysis of transformation-competent, replication-defective simian virus 40 large T antigen mutants.
... Several T-antigen activities, such as origin binding, viral DNA replication, ATPase activity, stimulation of cell DNA synthesis, stimulation of rDNA transcription, transformation, and nu[r] ... See full document
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Mutational analysis of simian virus 40 small-t antigen.
... 2895 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Several point mutations in the simian virus 40 SV40 small-t antigen have been analyzed for their effects on protein[r] ... See full document
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Intracellular forms of simian virus 40 nucleoprotein complexes. II. Biochemical and electron microscopic analysis of simian virus 40 virion assembly.
... Since the CsCl gradient procedure used by these authors is known to disrupt many nucleoprotein complexes, including adenovirus assembly intermediates 2, 3, it is not clear whether the em[r] ... See full document
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DNA infectivity and the induction of host DNA synthesis with temperature-sensitive mutants of simian virus 40.
... This, and the fact that mutants of the A group induce host DNA synthesis in CV-1 cells although they do not replicate viral DNA at 40 C 5, 18 suggested either that an early function othe[r] ... See full document
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Temperature dependency for maintenance of transformation in mouse cells transformed by simian virus 40 tsA mutants.
... The great majority of mouse embryo fibroblast transformants generated with tsA mutant virus were temperature sensitive for transformation in all three assays, regardless of the input mul[r] ... See full document
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Simian virus 40 mutants carrying extensive deletions in the 72-base-pair repeat region.
... 9a allow us to conclude that the deletions in the upstream region of the principal late SV40 initiation site L325, including the 72-bp repeat segment, do not interfere appreciably with t[r] ... See full document
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Replication and transformation functions of in vitro-generated simian virus 40 large T antigen mutants.
... Mutant of simian virus 40 large T-antigen that is defective for viral DNA synthesis, but competent for transformation of cultured rat cells.. Simian virus 40 deoxyribonucleic acid synthe[r] ... See full document
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Nucleotide sequence analysis of two simian virus 40 mutants with deletions in the region coding for the carboxyl terminus of the T antigen.
... The site of the deletion is indicated by a delta symboL B The subfragment containing the dektion in mutant d11265 and labeled at only one 5' end was generated by kination of the HindII +[r] ... See full document
6
Conditional Lethal Mutants of Adenovirus 2-Simian Virus 40 Hybrids I. Host Range Mutants of Ad2+ND1
... If Ad2+ND1 grows in monkey cells because it contains SV40 sequences, then viruses with mutations in the SV40 segment should comprise a class of host range mutants... describe here the is[r] ... See full document
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Simian virus 40 in humans
... polyoma- virus life cycle is less clear. Analysis of SV40 deletion mutants revealed that tag is not essential for lytic infection in culture ...increases virus yield in permissive cells ... See full document
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Viral DNA Synthesis in Cells Infected by Temperature-Sensitive Mutants of Simian Virus 40
... For the initial screening of mutants data in Table 1 the 24 well plates of Linbro Chemical Co., New 116 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Temperature-sens[r] ... See full document
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Spontaneous deletion mutants resulting from a frameshift insertion in the simian virus 40 agnogene.
... 1 0022-538X/86/040165-08$02.00/0 Copyright © 1986, American Society for Microbiology Spontaneous Deletion Mutants Resulting from a Frameshift Insertion in the Simian Virus 40 Agnogene SH[r] ... See full document
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Products of complementation between temperature-sensitive mutants of simian virus 40.
... These results imply that i the D product may be synthesized late in infection; ii therefore, any defect exhibited by D virions early in infection probably results from the introduction o[r] ... See full document
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Functional characterization of temperature-sensitive mutants of simian virus 40 large T antigen.
... Purified T antigens T ag were either not exposed - or exposed + to a heat shock for 10 min at 39°C, incubated in replication buffer containing plasmids with the SV40 origin of replicatio[r] ... See full document
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Physical and Genetic Characterization of Deletion Mutants of Simian Virus 40 Constructed In Vitro
... Mutant dl-1244 was also sensitive to BamHI endonuclease cleavage and yielded a normal HindII + III-G fragment; however, its deletion at map position 0.15 was confirmed by the fact that h[r] ... See full document
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Simian virus 40 mutants carrying two temperature-sensitive mutations.
... Double mutants of A:B, A:C, and A:BC complementation groups, like their A parent, were defective in viral DNA replication, in the induction of host DNA synthesis, and in the transformati[r] ... See full document
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Mutants deleted in the agnogene of simian virus 40 define a new complementation group.
... The data presented here and elsewhere 13, 15, 16 indicate that LP-1 i is a 61-amino acid, highly basic, acid-soluble, phosphorylated protein whose amino terminus is an unblocked valine r[r] ... See full document
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Functional complementation of nuclear targeting-defective mutants of simian virus 40 structural proteins.
... Since what was observed in host cells during infection by polyomaviruses 10, 13, 19, 22, 23, 26 does not reveal the role of individual protein components for nuclear targeting, these obs[r] ... See full document
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Construction and analysis of viable deletion mutants of simian virus 40.
... The SV40 map location and size of the deletions were determined by the Si nuclease mapping procedure 18 and from the change in mobility of fragments produced by Hind II+III endonuclease [r] ... See full document
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