[PDF] Top 20 trans-Complementation of Flavivirus RNA Polymerase Gene NS5 by Using Kunjin Virus Replicon-Expressing BHK Cells
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trans-Complementation of Flavivirus RNA Polymerase Gene NS5 by Using Kunjin Virus Replicon-Expressing BHK Cells
... predicted RNA polymerase motif GDD (producing FLdGDD) and of one of the predicted methyltransferase motifs (S-adenosylmethionine [SAM] binding site, producing FLdSAM) were introduced separately into ... See full document
10
Coupling between Replication and Packaging of Flavivirus RNA: Evidence Derived from the Use of DNA-Based Full-Length cDNA Clones of Kunjin Virus
... KUN RNA packaging, we prepared two plasmid DNA constructs, pKUN1 and pKUN1dGDD, which allowed production in transfected cells of the full-length replication- competent and replication-deficient RNAs, ... See full document
8
trans-Complementation of yellow fever virus NS1 reveals a role in early RNA replication.
... of BHK cells, has been published elsewhere ...positive-sense RNA viruses (39). Recombinant self- replicating (replicon) SIN RNAs can be used to drive expres- sion of heterologous genes in ... See full document
10
Complementation Analysis of the Flavivirus Kunjin NS3 and NS5 Proteins Defines the Minimal Regions Essential for Formation of a Replication Complex and Shows a Requirement of NS3 in cis for Virus Assembly
... BHK cells (see Table 1 in reference ...the complementation efficiencies of these two RNAs with that of the RNA containing combined triple dele- tions in NS1, NS3, and NS5 genes due to ... See full document
10
Subgenomic replicons of the flavivirus Kunjin: construction and applications.
... Several Kunjin virus (KUN) subgenomic replicons containing large deletions in the structural region (C-prM-E) and in the 3 * untranslated region (3 * UTR) of the genome have been ...constructed. ... See full document
9
cis- and trans-Acting Elements in Flavivirus RNA Replication
... E-positive cells and no multicellular E-positive foci were observed at day 4 after transfection ...complemented RNA from day 2 to day 4 after transfection was detected by Northern blot analysis ... See full document
11
Translation of the Flavivirus Kunjin NS3 Gene in cis but Not Its RNA Sequence or Secondary Structure Is Essential for Efficient RNA Packaging
... studies using trans-complementation analysis of Kunjin virus (KUN) full-length cDNA clones harboring in-frame deletions in the NS3 gene demonstrated the inability of these ... See full document
10
Analysis of Adaptive Mutations in Kunjin Virus Replicon RNA Reveals a Novel Role for the Flavivirus Nonstructural Protein NS2A in Inhibition of Beta Interferon Promoter-Driven Transcription
... stable BHK cell lines. BHK cells (total cell population) transfected with the wt or each of the mutated replicon RNAs were propagated in medium with 5 g of puromycin/ml for four passages ... See full document
11
Efficient trans-Complementation of the Flavivirus Kunjin NS5 Protein but Not of the NS1 Protein Requires Its Coexpression with Other Components of the Viral Replicase
... Successful trans-complementation of the defective Kunjin virus (KUN) RNA FLdGDD with a deletion of the RNA polymerase motif GDD in the NS5 gene by ... See full document
9
trans-Complementation Analysis of the Flavivirus Kunjin ns5 Gene Reveals an Essential Role for Translation of Its N-Terminal Half in RNA Replication
... the flavivirus NS5 protein interacts via conserved motifs (a, b, and c) with NS3 and possibly other NS proteins such as NS2A during translation to initiate formation of the RC, most likely on the 3⬘UTR of ... See full document
9
Encapsidation of the Flavivirus Kunjin Replicon RNA by Using a Complementation System Providing Kunjin Virus Structural Proteins in trans
... SFV replicon, and we focused next on expressing prME ...prME replicon. It was shown in a number of flavivirus studies that expression of a prME cassette either from recombinant vaccinia ... See full document
11
Molecular Function Analysis of Rabies Virus RNA Polymerase L Protein by Using an L Gene-Deficient Virus
... the trans-complementation assay by using a functionally defective L protein ...NNS RNA viruses (13, 27, ...by using Nishi-ΔL/Nluc: we measured luciferase activity produced by ... See full document
16
trans rescue of a mutant poliovirus RNA polymerase function.
... Our interpretation is that the 3D-18 polymerase synthesized at 37 or 39°C or shifted from 33 to 39°C is misfolded and that this misfolding accounts for both the low level of in vitro elo[r] ... See full document
11
Nairovirus RNA Sequences Expressed by a Semliki Forest Virus Replicon Induce RNA Interference in Tick Cells
... to virus infection and has been illustrated in several viral infection ...negative RNA genome like dengue virus, La Crosse virus, or Rift Valley fever virus (7, 14, ...The cells ... See full document
6
trans complementation by RNA of defective foot-and-mouth disease virus internal ribosome entry site elements.
... The strangest result that we observed was the enhancement of CAT expression directed by the defective element lacking domain 2 when coexpressed with the antisense transcript of the FMDV [r] ... See full document
7
Generation and characterization of a recombinant Rift Valley fever virus expressing a V5 epitope tagged RNA dependent RNA polymerase
... itis virus internal ribosome entry site ...the virus 39 UTR, thereby excising the NSs coding ...by using a PCR mutagenesis approach (Shi & Elliott, ... See full document
8
Morphology of BHK-21 Cells Infected with Sindbis Virus Temperature-Sensitive Mutants in Complementation Groups D and E
... Cells infected at nonpermissive temperature with virus defective in complementation group E had nucleocapsids attached in large numbers to the inside surface of the host plasma membrane.[r] ... See full document
5
Efficient Rescue of Hepatitis C Virus RNA Replication by trans-Complementation with Nonstructural Protein 5A
... for trans-complementation of the pestivirus BVDV ...by trans-complemen- tation. However, in that study complementation only was pos- sible when mutants were transfected into cell clones that ... See full document
BHK cells expressing Sindbis virus-induced homologous interference allow the translation of nonstructural genes of superinfecting virus.
... We attempted to determine whether superinfecting virus fused into the cytoplasm of cells with established homologous interference could express early functions related to RNA synthesis a[r] ... See full document
7
Interactions between the Dengue Virus Polymerase NS5 and Stem-Loop A
... of RNA replication by dengue virus is still not completely understood despite the significant progress made in the last few ...dengue virus replication, but quantitative analysis of the interactions ... See full document
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