[PDF] Top 20 Complementation studies with Rous sarcoma virus gag and gag-pol polyprotein mutants.
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Complementation studies with Rous sarcoma virus gag and gag-pol polyprotein mutants.
... These results show i that RSV PR can activate itself from the gag-pol polyprotein as is the case for mammalian retroviruses, since we have shown that an unprocessed gag-pol polyprotein d[r] ... See full document
6
The membrane-binding domain of the Rous sarcoma virus Gag protein.
... Retroviral Gag proteins play dual roles in the virus life ...thereafter, Gag is cleaved by the viral protease (PR) to yield the major structural components of the virion (matrix [MA], capsid [CA], ... See full document
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Analysis of Rous sarcoma virus Gag protein by mass spectrometry indicates trimming by host exopeptidase.
... precursor polyprotein encoded by the gag gene. Cleavage of the Gag polyprotein leads to a morphological change in the virus par- ticle known as maturation and is essential for ...avian ... See full document
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Analysis of cleavage site mutations between the NC and PR Gag domains of Rous sarcoma virus.
... site mutants like ...a Gag fragment of about 63 kDa, plus an N-terminally truncated, inactive ...CA Gag proteins (3) and of mature RT and IN Pol proteins (32) in cs22 particles to be due to ... See full document
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An LYPSL Late Domain in the Gag Protein Contributes to the Efficient Release and Replication of Rous Sarcoma Virus
... in Gag, is a secondary late do- ...of Gag release with pulse-chase labeling, the rate of viral spread with flow cytom- etry, and the number of budding structures on the surface of cells with SEM, we were ... See full document
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Role of RNA in Facilitating Gag/Gag-Pol Interaction
... The Gag precursor in human immunodeficiency virus type 1 (HIV-1) is alone sufficient to produce viral particles (14, 21, 28, ...between Gag mol- ecules have been delineated, mainly in the C-terminal ... See full document
7
Genetic analysis of the major homology region of the Rous sarcoma virus Gag protein.
... release, mutants of Rous sarcoma virus were created in which segments of CA were deleted or single conserved residues in the MHR were ...deletion mutants to release particles at rates ... See full document
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Altered Rous sarcoma virus Gag polyprotein processing and its effects on particle formation.
... polyproteins, Gag and Gag-Pol, which migrate to the plasma ...of Gag referred to as the membrane binding (M) ...of Gag polyproteins (Gag and Gag-Pol) is necessary ... See full document
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Evidence for a second function of the MA sequence in the Rous sarcoma virus Gag protein.
... the Gag protein is the only viral gene product required for this ...ribosomes, Gag polypro- teins are transported to the plasma membrane, where 2,000 to 3,000 molecules (45) aggregate to initiate particle ... See full document
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Detailed Mapping of the Nuclear Export Signal in the Rous Sarcoma Virus Gag Protein
... RSV Gag polyprotein traf- fics through the nucleus of infected cells ...cytoplasm, Gag is imported into the nucleus, likely through the use of a nuclear import signal within the MA ...of Gag ... See full document
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Characterization of Rous Sarcoma Virus Gag Particles Assembled In Vitro
... of Gag is inferred to play the central role in the specific recognition of psi, the RNA packaging sequence, and the Cys-His motifs are essential for this packaging function (reviewed in reference ...NC ... See full document
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Conditions for Copackaging Rous Sarcoma Virus and Murine Leukemia Virus Gag Proteins during Retroviral Budding
... both Gag proteins were immunopre- cipitated with either antiserum, indicating that direct interac- tions had taken place between them ...RSV Gag was less than MLV Gag in this particular ... See full document
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Role of Nedd4 and Ubiquitination of Rous Sarcoma Virus Gag in Budding of Virus-Like Particles from Cells
... the Gag-ubiquitin (Gag-Ub) coding region was subcloned from pGag-Ub (14) and inserted into ...The Gag-Ub coding sequence was amplified by PCR to introduce a KpnI site at the 5 ⬘ end and a BsrGI site ... See full document
11
Mutational analysis of the gag-pol junction of Moloney murine leukemia virus: requirements for expression of the gag-pol fusion protein.
... To explore the cis-acting sequence requirements for the readthrough event in vivo, we generated a library of mutants of the Moloney murine leukemia virus with point mutations near the te[r] ... See full document
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Efficiency and selectivity of RNA packaging by Rous sarcoma virus Gag deletion mutants.
... To gain information about which portions of Gag is required for RNA packaging in the avian sarcoma and leukemia virus system, we utilized a series ofgag deletion mutants that retain the [r] ... See full document
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Nucleotide sequence of the 5' noncoding region and part of the gag gene of Rous sarcoma virus.
... Each of these roles involves structural features located in the vicinity of the 5' terminus of the viral genome: the initiation site for reverse transcription 41; a nucleotide sequence R[r] ... See full document
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Proposed gag-encoded transcriptional activator is not necessary for Rous sarcoma virus replication or transformation.
... Nucleotide sequence and transcriptional activity of the caprine arthritis Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest found no difference in expression of viral RNA[r] ... See full document
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Creation and expression of myristylated forms of Rous sarcoma virus gag protein in mammalian cells.
... The primary purpose of the experiments described here was to determine whether particle formation by the RSV gag product, Pr76, could be promoted in mammalian cells by the creation of a [r] ... See full document
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Myristylation of Rous sarcoma virus Gag protein does not prevent replication in avian cells.
... We found that myristylated derivatives of the Rous sarcoma virus Gag protein are fully functional for particle formation in avian cells and that the addition of myristic acid has very li[r] ... See full document
5
Necessity of the spacer peptide between CA and NC in the Rous sarcoma virus gag protein.
... 10 NOTES Necessity of the Spacer Peptide between CA and NC in the Rous Sarcoma Virus Gag Protein Received 3 May 1993/Accepted 25 June 1993 A mutant of Rous sarcoma virus was constructed [r] ... See full document
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