[PDF] Top 20 Construction of Double Gene Expression Vector
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Construction of Double Gene Expression Vector
... larger vector. In order to overcome these problems a novel expression vector was developed with unique restriction sites and two different inducible promoters to control the expression of both ... See full document
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Construction of interference vector targeting <em>Ep-CAM</em> gene and its effects on colorectal cancer cell proliferation
... RNA-mediated gene silencing method to suppress the Ep-CAM gene in colorectal cancer cell lines and to study its role in colorectal ...Ep-CAM gene in colorectal carcinoma cells. The messenger (m)RNA ... See full document
6
Construction, characterisation and utilisation of a novel E coli expression vector
... To obtain high expression of a cloned gene transcription should be maximised using a strong promoter. A promoter is a DNA sequence that directs DNA dependent RNA polymerase to bind to DNA and to initiate ... See full document
278
Construction of Overexpression Vector of ACBP Gene in Zygosaccharomyces rouxii
... tion, which make it contains the same restriction enzyme site. And then let the gene fragment was connected with expression vector, recombination plasmid transformated state feelings E. coli . ... See full document
8
Expression Vector Construction and Genetic Transformation of Rosa rugosa β l,3 Glucanase Gene (RrGlu)
... The RrGlu fragment with Bamh I and Xba I ends was cloned into a PBI121 vec- tor. The obtained plasmid was transferred into E. coli DH5a. After the restric- tion ends were confirmed by the double digestion method, ... See full document
7
CONSTRUCTION OF YEAST EXPRESSION VECTOR CONTAINING ELT-B GENE FOR HIGH LEVEL OF HEAT LABILE ENTEROTOXIN-B SUBUNIT (LT-B)
... as vector carrying eltB fragment and expected recombinant protein is expressed in intra ...of gene expression is strictly related to specific codons 2 ...the expression levels of prokaryotic ... See full document
6
Cloning and expression of human vasohibin1 gene in E. coli
... pET28a expression vector which transformed into ...an expression inducer in ...and double digestion with EcoRI and HindIII restriction ...of gene inhibition was carried out through ... See full document
5
Construction of Retroviral Vectors with Improved Safety, Gene Expression, and Versatility
... of gene expression from several different types of retroviral vectors currently used in gene therapy clinical trials ...retroviral vector was superior in con- ferring gene ... See full document
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EXPRESSION OF NONSTRUCTURAL GLYCOPROTEIN NSP4 OF SAIl SIMIAN ROTAV IRUS IN ESCHERICHIA COLI AND PRODUCTION OF ANTIBODY AGAINST IT
... sequencing. Construction of expression plasmid containing nsp4 gene was performed and expression of NSP4 was demonstrated by SDS PAGE, Western blot, ELISA and IF using polyclonal antibody ... See full document
8
Design and Construction of a Cloning Vector Containing the hspX Gene of Mycobacterium tuberculosis
... Heat shock protein X (hspX) has been purified from M. tuberculosis. Heat shock protein X has a proposed role in the maintenance of long-term viability during latent, asymptomatic infections, and in replication during ... See full document
5
BmICE-2 interference vector construction and its silencing effect in the BmN-SWU1 cells
... The designed microRNA-siBmICE-2 and microRNA-siBmICE-2-control sequences were synthesized by Gene script (Nanjing, China). Synthesized microRNA-siBmICE-2, microRNA-siBmICE-2-control and PIZ/V5-dsRed plasmids were ... See full document
8
Construction of a recombinant eukaryotic expression vector containing <em>DNM3</em> gene and its expression in colon cancer cells
... recombinant vector PCDH-CMV- MCS-EF1a-GFP-Puro-DNM3, the digested and purified PCR products and plasmids were ligated with DNA Ligation Kit ...recombinant vector was transformed into competent ... See full document
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Construction of a recombinant eukaryotic expression vector containing PHD3 gene and its expression in HepG2 cells
... So far, the relationship between PHD3 and hepatocel- lular cancer (HCC) is still unclear. To clarify the effect of PHD3 on HCC, we constructed a recombinant eukar- yotic expression vector containing PHD3 ... See full document
7
Antibiotic-free selection in E. coli: new considerations for optimal design and improved production
... essential gene making use of an expression vector in a strain with a defect or inhibited expression of the same essential ...dapD gene, which has a role in the Lysine biosynthetic ... See full document
10
Construction of a Shuttle Vector for Heterologous Gene Expression in Escherichia coli and Microalgae Anabaena
... desired gene expression and (c) neomycin phosphotransferase gene with its own promoter for the selection of transfor- ...constructed vector pAnFP was evaluated by cloning, transfer and ... See full document
5
Expression Vector Construction and Genetic Transformation of Paeonia lactiflora Gibberellin 20 Oxidase Gene
... overexpression vector of the PlGA 20 ox gene was success- fully constructed and the function was explored in this study, which will un- doubtedly facilitate the genetic transformation of herbaceous ...ox ... See full document
10
Construction and transformation of expression vector containing Panax japonicus SS gene.
... SS gene, the plant expression vectors pCXSN-PjSS and pCXSN-antiPjSS were constructed and transformed into Agrobactria ...Hyg gene in resistant ... See full document
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Seeking gene relationships in gene expression data using support vector machine regression
... in gene expression have been located using linkage and association ...of expression variation as a quantitative phenotype reflects its underlying genetic ...support vector machine regression ... See full document
8
Expression and immunogenicity of erythropoietin gene cloned in eukaryotic expression vector (pVAX1 vector)
... Primary polyclonal antibody against the human erythropoietin gene was raised in mouse by hyper immunization of six mice with pVAX1.epo.hu plasmid. 50 µg of rplasmid DNA was injected intramuscularly in lateral ... See full document
7
Identification of a Functional Envelope Protein from the HERV-K Family of Human Endogenous Retroviruses
... ENV expression vector and either a control plasmid (CMV  , without SIV) or the SIV plasmids (see panel A; with SIV) were concentrated and analyzed by SDS-PAGE as described for panel ... See full document
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