[PDF] Top 20 Coreceptor Utilization by Human Immunodeficiency Virus Type 1 Is Not a Primary Determinant of Neutralization Sensitivity

... tributed reagents for this work: Dan Littman (HHMI, NYU Medical Center), Thera Mulvania and Jim Mullins (University of Washington) for U87-CD4 cell lines, Irvin Chen (UCLA School of Medicine) for pNLthyDBgl provirus, ... See full document

... to neutralization by gp120 vaccine sera that neutralize TCLA viruses (cited in reference, 9), several alternative explanations have been proposed, but none has been entirely ...TCLA virus in the absence of ... See full document

... of human immunodeficiency virus ...in coreceptor utilization have also been noted upon adaptation of primary isolates (PI) to growth in established T-cell ...the ... See full document

... rapid neutralization assay end point (16, 21, 66), FACS is not a procedure that lends itself to the routine processing of up to hundreds of samples, as can be needed in clinical studies of HIV-1 ...with ... See full document

... Resistance of primary isolates of human immunodeficiency virus type 1 to neutralization by soluble CD4 is not due to lower affinity with the viral envelope glycoprotein gpl20.. Human imm[r] ... See full document

... and sensitivity to neutralization by soluble CD4 and monoclonal antibodies were examined for molecularly cloned envelope glycoproteins derived from human immunodefi- ciency virus type ... See full document

... to neutralization by envelope glycoprotein- directed ...the virus-cell interface (46, 81, ...with primary HIV-1, induce dra- matic increases in sensitivity to neutralization by ... See full document

... the neutralization of PI may be largely ...cross-clade neutralization (6, 10, 36, 49), but antibodies with these speci- ficities are rarely ...in coreceptor binding is exposed once CD4 is ...crucial ... See full document

... ⫽ 1 neutralization stoichiometry is shared by all nine mono- clonal antibodies studied in this report; these antibodies ex- hibit a range of neutralization potencies and bind distinct el- ements on ... See full document

... CXCR4 coreceptor, is a mere reflection of the collapse of the immune system, which finally allows uncon- trolled ...rapid/high virus leads to a rapid loss of CD4 1 lymphocytes (18), it is tempting to ... See full document

... sCD4-17b neutralization against genetically di- verse HIV-1 ...expressing coreceptor but no CD4, Envs from genetically di- verse isolates (clades A through F) were all potently inhibited by MAb 17b ... See full document

... of virus neutralization by sera from infected individuals, coreceptor usage probably does not de- termine the ability of HIV-1 to be neutralized by ...used human monoclonal antibodies ... See full document

... that human immunodeficiency virus type 1 (HIV-1) evolves toward increased cytopathicity in conjunction with disease progression in infected ...is coreceptor ... See full document

... factor 1 is produced at relatively high concen- trations by cells in the stroma of lymphoid tissues and so is able to specifically prevent HIV-1 interactions with CXCR4 ... See full document

... potent human MAbs (IgG1b12, 2G12, and 2F5) was as- ...FIG. 1. Envelope glycoproteins from distinct HIV-1 strains exhibit very different sensitivities to neutralization by anti-HIV-1-Env ... See full document

... of coreceptor utilization during the course of HIV-1 infection represents a key mechanism for extension of the spectrum of target ...major determinant of tropism (8, 11, 30, 31, 43, 57, 65, ... See full document

... fected primary PBMC and ...Env-pseudotyped virus and lowest for the clade F ...CD4 1 human osteosarcoma cells ...CC-CKR5 coreceptor for ...on coreceptor usage by divergent ... See full document

... MAbs and polyclonal Abs. Five polyclonal serum samples and two MAbs were used in the neutralization tests. Sera F and N are from asymptomatic HIV- positive volunteers from the Veterans Affairs Medical Center (New ... See full document

... X4 tropism (26, 32, 41, 42, 54), our findings support the im- portance of a positively charged V3 for this phenotype. How- ever, the clustering of these residues raises interesting issues in view of recently proposed ... See full document

... the coreceptor specificity of these related species, since the V3 sequences of the R5 env variants did not differ from the highly charged V3 sequence of ... See full document