[PDF] Top 20 Correcting temperature sensitive protein folding defects
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Correcting temperature sensitive protein folding defects
... using protein stabilizing agents in vivo to correct protein folding defects has been in the scientific litera- ture for 30 yr or ...growth temperature or by increasing the osmotic ... See full document
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Characterization of two temperature-sensitive mutants of coronavirus mouse hepatitis virus strain A59 with maturation defects in the spike protein.
... membrane protein that forms the characteristic coro- navirus ...membrane protein M and the small membrane pro- tein ...structural protein, N, encapsidates the viral ... See full document
7
Molecular Defects Caused by Temperature-Sensitive Mutations in Semliki Forest Virus nsP1
... similar defects in minus-strand synthesis ...the protein it is a difficult problem to approach, but some clues have emerged from numerous biochemical and genetic ... See full document
9
Enzymatic Defects of the nsP2 Proteins of Semliki Forest Virus Temperature-Sensitive Mutants
... Protease defects. The in vitro protease assay using purified nsP2 proteins and purified thioredoxin fusion protein Trx34 containing the polyprotein cleavage site between nsP3 and nsP4 showed that all of the ... See full document
12
Functional defects of RNA-negative temperature-sensitive mutants of Sindbis and Semliki Forest viruses.
... At the same time a large nonstructural protein, about 200,000 daltons, accumu19 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Defects in RNA and protein synthesis of [r] ... See full document
11
A Novel Mutation in Murine Hepatitis Virus nsp5, the Viral 3C-Like Proteinase, Causes Temperature-Sensitive Defects in Viral Growth and Protein Processing
... FIG. 5. Structural models of MHV nsp5s from WT, Alb/ts/nsp5/V148A, nsp5/S133N/V148A, and nsp5/H134Y/V148A viruses. A homology model of the MHV nsp5 protein was generated with the program 3D-Jigsaw using the ... See full document
10
Chang, Hung-Chun (2007): Mechanisms of de novo multi-domain protein folding in bacteria and eukaryotes. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... kDa protein, which binds to ribosomes at a 1:1 stoichiometry and interacts with nascent chains as short as 57 residues (Hesterkamp et ...subsequent folding (Deuerling et ...apparent folding ... See full document
146
A Mutation in the Gene-encoding Bacteriophage T7 DNA Polymerase That Renders the Phage Temperature-sensitive
... the protein synthesized at elevated tem- peratures is a kinetically trapped intermediate in the folding pathway of T7 DNA polymerase that does not attain the native conformation, rather than a mutant with ... See full document
11
The Major Attenuating Mutations of the Respiratory Syncytial Virus Vaccine Candidate cpts530/1009 Specify Temperature-Sensitive Defects in Transcription and Replication and a Non-Temperature-Sensitive Alteration in mRNA Termination
... nonstructural protein (NS)1 gene including the gene start (GS) transcription signal; the CAT sequence is flanked on the downstream end by the downstream nontranslated region of the L gene including the ... See full document
5
Analysis of the defects of temperature-sensitive mutants of vesicular stomatitis virus: intracellular degradation of specific viral proteins.
... Certain viral proteins encoded by the mutant strains, usually the putative mutant protein for the assigned complementation group, were shown to be degraded more rapidly at the nonpermiss[r] ... See full document
9
Genetic properties of temperature-sensitive folding mutants of the coat protein of phage P22.
... Temperature-sensitive mutations at 17 sites in the gene for the coat protein of Phage P22 are of the latter class, preventing the productive folding of the polypeptide chain at[r] ... See full document
12
A Single Mutation Responsible for Temperature-Sensitive Entry and Assembly Defects in the VP1-2 Protein of Herpes Simplex Virus
... Characterization of virus yield and protein expression. We measured the progression of infection at the level of the accu- mulation of representative viral proteins, ICP8 and VP5, ini- tially at a single time ... See full document
13
Protein aggregation in disease: a role for folding intermediates forming specific multimeric interactions
... more sensitive to heat treat- ment, which leads to the formation of fibrils with a pre- dominantly β ...mutant protein pop- ulates an amyloidogenic ...physiologic temperature, the mutant pro- teins ... See full document
13
TEMPERATURE-SENSITIVE MUTANTS BLOCKED IN THE FOLDING OR SUBUNIT ASSMBLY OF THE BACTERIOPHAGE P22 TAILSPIKE PROTEIN. I. FINE-STRUCTURE MAPPING
... Studies of the defective phenotypes of the ts mutants (GOLDENBERG and KING 1981; SMITH and KING 1981) revealed that most do not affect the thermostability of the mat[r] ... See full document
22
Fast folding dynamics of proteins and peptides
... absorption temperature jump ...the protein denatured ...with temperature [129, 134] ...the protein WT-BBL is similar to the nanosecond lifetime observed for the dansylated BBL [129] ...a ... See full document
138
Endoplasmic reticulum stress and oxidative stress: A vicious nexus implicated in bowel disease pathophysiology
... of protein synthesis is compensated for by these secretory cells via advanced protein regulatory mechanisms, which optimize protein folding and the elimination of misfolded proteins ...the ... See full document
19
Identification of temperature-sensitive mutants of vaccinia virus that are defective in conversion of concatemeric replicative intermediates to the mature linear DNA genome.
... I show the use of PFGE as a tool to rapidly detect and characterize replication defects among temperature-sensitive mutants of vaccinia virus and show the existence of several classes of[r] ... See full document
8
Intragenic suppressors of folding defects in the P22 tailspike protein.
... and KING 1988). These criteria indicated that mis- sense proteins with ts and lethal phenotypes generated from amber mutants were appropriate starting alleles for selection[r] ... See full document
15
Oxidative protein folding in bacteria
... The in vitro reconstitution of the disulphide bond cataly- sis system using purified components verified the con- nection between electron transport and disulphide bond formation (Bader et al., 1999). DsbB transfers its ... See full document
8
Protein folding and chart parsing
... the folding process is guided by a hierarchical search strategy, whereby folding begins simultaneously and independently in different parts of the chain, leading initially to the formation of local ... See full document
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