[PDF] Top 20 Critical spatial requirement within the origin of simian virus 40 DNA replication.

... DNA binding studies showed that subunits of the simian virus 40 A protein T antigen bound to each of the four recognition pentanucleotides in the origin palindrome but did so with reduce[r] ... See full document

... Using ecoRI and hpaII restriction endonucleases, each of which can cleave SV40 DNA once at a known location 10, 11, 12, 14, the bound protein can be localized at 0.7 i 0.05 on the SV40 D[r] ... See full document

... single-stranded DNA binding domain of large T antigen to amino acid residues 259 to ...support simian virus 40 (SV40) replication and to bind to single-stranded ...Two ... See full document

... 5F), again confirming a very high degree of preexisting nega- tive supercoiling for this SV40 topoisomer. Increasing the chlo- roquine concentration to 300 mg/ml caused a significant reduc- tion of form U mobility (data ... See full document

... SV40 DNA beside ...plasmid DNA has entered the nucleus and that no replication has taken ...place. DNA is present in the cytoplasm ...arrow) within the depth of the optical ...the ... See full document

... Analysis of A protein binding to a series of nonviable mutanfts progressively deleting these contact sites leads to the following conclusions: i stable binding of subunits of A protein t[r] ... See full document

... with DNA is ...of DNA whereas a DNA-associated hex- amer had no chirality and appeared ...in origin unwinding, ...melted/unwound DNA as the individual subunits assemble to form a ... See full document

... We have also used DNA footprinting techniques to show indirectly that the assembly of T antigen leads to stepwise and cooperative binding to the early and late halves of the SV40 core or[r] ... See full document

... Simian virus 40 SV40 large tumor antigen causes stepwise changes in SV40 origin structure during initiation of DNA replication.. Molecular cloning: a laboratory manual, 2nd ed.[r] ... See full document

... viral DNA repli- cation and transformation (6, 18, 47, ...an origin binding domain (OBD) and a C-terminal helicase ...and virus replication, was solved in complex with a portion of the ... See full document

... To investigate the specificity of pseudorevertant T antigens for different ori sequences, we constructed in vitro recombinants that contained mutant T antigen genes and various sequences[r] ... See full document

... The presence of the SV40 control region, including the origin, enhancer, and promoter sequences, was sufficient to cause genomic instability at the site of integration of pSV2neo in five[r] ... See full document

... LEWTON,1 ROBERT TJIAN,2 AND PETER TEGTMEYERl* Received 4 October 1982/Accepted 10 January 1983 Investigation of the DNA binding properties of the simian virus 40 SV40 A protein large T a[r] ... See full document

... Footprint and fragment assays led to the following conclusions: i in some cases a single recognition pentanucleotide is sufficient to direct the binding and accurate alignment of A prote[r] ... See full document

... Replicating deoxyribonucleic acid DNA definitive analysis of the origin and direction of molecules of simian virus 40 SV40 are small, replication could not be made.. In the present circu[r] ... See full document

... Muntjac cells resulted in significant differences To summarize, in the entire population, initiain the U statistic when compared with serum- tion sites were separated by shorter distance[r] ... See full document

... Since SV40 DNA replication absolutely depends on SV40 T antigen, anti-T serum from a tumor-bearing hamster was added to the reaction mixture containing both HeLa nuclear extract and CosI[r] ... See full document

... Isolated nuclei as well as nuclear matrices were able to continue SV40 DNA elongation under replication conditions in situ, arguing for a coordinated and functional association of SV40 D[r] ... See full document

... the virus, in many cases by interacting with cellular proteins and modulating their activity ...viral DNA replication (18), and defective virion assembly ...adenovirus replication in monkey ... See full document

... Footprinting of the insertion mutants and the four pentanucleotide mutants strongly suggests that the early and late halves of the origin function as distinct entities in formation of th[r] ... See full document