[PDF] Top 20 Deletion of the VP16 open reading frame of herpes simplex virus type 1.
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Deletion of the VP16 open reading frame of herpes simplex virus type 1.
... Deletion mutant virions propagated in this cell line contained wild-type, cell-derived VP16 protein that was recruited during virion assembly and was functional for immediate-early gene [r] ... See full document
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Effects of Simultaneous Deletion of pUL11 and Glycoprotein M on Virion Maturation of Herpes Simplex Virus Type 1
... Although deletion of either gene had only moderate effects on replication of the related alphaherpesviruses herpes simplex virus type 1 (HSV-1) and pseudorabies ... See full document
12
Expression of herpes simplex virus type 1 glycoprotein D deletion mutants in mammalian cells.
... First, each contained highermolecular-weight or aggregated forms; second, processing of the N-linked oligosaccharides to the product form was markedly reduced and no 0-linked oligosaccha[r] ... See full document
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In vivo deletion analysis of the herpes simplex virus type 1 latency-associated transcript promoter.
... recombinant virus KOS/1 has been described previously ...The deletion series was constructed as outlined in ...17 1 (16). KOS/1 has been renamed ...the deletion series were named ... See full document
7
An avirulent ICP34.5 deletion mutant of herpes simplex virus type 1 is capable of in vivo spontaneous reactivation.
... approximately equivalent to the amount of TG DNA in each experimental sample. The DNA was then subjected to PCR and Southern analysis as described in Materials and Methods. The counts per minute for the HSV-1 PCR ... See full document
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Physical mapping of the herpes simplex virus type 2 nuc- lesion affecting alkaline exonuclease activity by using herpes simplex virus type 1 deletion clones.
... * 237 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The nuc- lesion affecting alkaline exonuclease activity in the herpes simplex virus type 2 HSV-2 mutant ts1348 had[r] ... See full document
5
The promoter and transcriptional unit of a novel herpes simplex virus 1 alpha gene are contained in, and encode a protein in frame with, the open reading frame of the alpha 22 gene.
... by herpes simplex virus type 1 (HSV-1) ...protein VP16, which, in conjunction with cellular proteins, binds to it and transactivates the downstream gene after infection ... See full document
7
Varicella-zoster virus open reading frame 61 protein is functionally homologous to herpes simplex virus type 1 ICP0.
... Deletion mutants in the gene encoding the herpes simplex virus type 1 immediate-early protein ICPO exhibit impaired growth in cell culture.. GAL4-VP16 is an unusually potent transcriptio[r] ... See full document
6
The Herpes Simplex Virus Type 1 UL3 Transcript Starts within the UL3 Open Reading Frame and Encodes a 224-Amino-Acid Protein
... ⬃ 1-kb transcript that initiates at position 10974, which is within the UL3 ...UL3 deletion virus CB8116 expresses viral proteins UL1, UL2, and UL4 and is thus well suited for future studies on the ... See full document
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The regulation of synthesis and properties of the protein product of open reading frame P of the herpes simplex virus 1 genome.
... as VP16) (4, 20, 26, 34, ...as deletion of promoters and of the sequences en- coding the stable introns does not affect the establishment or maintenance of the latent state (16, 32, ... See full document
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Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1.
... sites of a glutathione S-transferase (GST) fusion protein expression vector pGEX-KG (16). Escherichia coli cells transformed with this plasmid were in- duced with 0.1 mM IPTG (isopropyl- b - D -thiogalactopyranoside) for ... See full document
9
Expression of a herpes simplex virus 1 open reading frame antisense to the gamma(1)34.5 gene and transcribed by an RNA 3' coterminal with the unspliced latency-associated transcript.
... The 8.3-kb transcript was detected by hybridization of electrophoretically separated RNA extracted from productively infected cells and from rabbit trigeminal ganglia harboring latent vi[r] ... See full document
8
Identification and characterization of the herpes simplex virus type 1 protein encoded by the UL37 open reading frame.
... Protein extracts from the purified HSV-1 virions were separated on SDS-polyacrylamide gels, and the protein bands were probed in immunoblots with antisera directed against the UL37, ICP8[r] ... See full document
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Phenotypic properties of herpes simplex virus 1 containing a derepressed open reading frame P gene.
... or ORF P 11 and incubated for 14 h at 37 8 C. Cells were fixed and reacted first with rabbit antiserum to GST-ORF P and then with goat anti-rabbit IgG conjugated to Texas red. Cells infected with ORF P11 showed distinct ... See full document
8
The size and symmetry of B capsids of herpes simplex virus type 1 are determined by the gene products of the UL26 open reading frame.
... Following infection of nonpermissive cells, the remaining capsid proteins VP5, VP19C, VP23, and VP26 interact to form sheets or spirals of capsid shells that lack the normal icosahedral [r] ... See full document
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Varicella-zoster virus open reading frame 10 protein, the herpes simplex virus VP16 homolog, transactivates herpesvirus immediate-early gene promoters.
... Furthermore, cell lines stably expressing ORF10 complement the HSV-1 mutant in1814, which lacks the transactivating function of VP16, and enhance the de novo synthesis of infectious viru[r] ... See full document
8
A conserved open reading frame that overlaps the herpes simplex virus thymidine kinase gene is important for viral growth in cell culture.
... Nucleotide sequence of the herpes simplex virus type 2 HSV-2 thymidine kinase gene and predicted amino acid sequence of thymidine kinase polypeptide and its comparison with the HSV-1 thy[r] ... See full document
5
Characterization of a temperature-sensitive mutant of the UL15 open reading frame of herpes simplex virus 1.
... In this series of experiments, replicate 25-cm2 cultures of Vero cells were infected in duplicate with the parent, mutant, or rescued virus 5 PFU per cell and incubated in a 34°C incubat[r] ... See full document
7
Identification and characterization of the herpes simplex virus type 1 virion protein encoded by the UL35 open reading frame.
... The 12-kDa protein recognized by the TrpE-UL35 antiserum was associated with purified HSV-1 virions and type A and B capsids, suggesting that the UL35 ORF may encode the 12-kDa capsid pr[r] ... See full document
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The U(L)15 gene of herpes simplex virus type 1 contains within its second exon a novel open reading frame that is translated in frame with the U(L)15 gene product.
... (17). Type A capsids con- sist of an icosahedral shell surrounding an electron-lucent ...core. Type B capsids resemble type A capsids but contain an internal circular structure or scaffold, whereas ... See full document
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