[PDF] Top 20 Rep-Dependent Initiation of Adeno-Associated Virus Type 2 DNA Replication by a Herpes Simplex Virus Type 1 Replication Complex in a Reconstituted System

... the replication complex. These results suggest that the DNA replication in this assay is not fully processive and that substantial displacement of the rep- lication complex is ... See full document

... to DNA (9, 73). In the absence of ICP4, the HSV-1 life cycle is arrested at the immediate-early phase ...the virus is still replication competent but grows poorly and also reactivates from ... See full document

... of Rep and ICP8, HeLa cells were cotransfected with plasmids specifying wt AAV-2 with and without the ITRs and cotransfected with expression plasmids for the minimal four HSV helper genes, UL29, UL5, UL8, ... See full document

... nome replication, HeLa shRad50 cells were transiently comple- mented with a wt or mutated MRN complex and assayed for their ability to support AAV ...incompletely reconstituted despite having the ... See full document

... helicase-primase complex (UL8) and the DNA polymerase processivity factor ...origin DNA, it has been postulated that OBPC may play a role in HSV DNA replication ...viral DNA ... See full document

... strong Rep-mediated ...to Rep-dependent repression ...of Rep interaction with the p5 RBE element (direct and indirect), where the contextual arrangement of promoter ele- ments in the p5IEE are ... See full document

... the replication of the HSV-1 replicon to some extent ...reporter DNA by determining the degree of colocalization of the LacI-EYFP and the Rep-Alexa Fluor 594 signals in the merged images of ... See full document

... ternary- complex formation, a series of Rep mutants ...ssDNA-dependent complex forma- tion was maintained with the single-amino-acid exchange mutant ...precluding DNA helicase activity ... See full document

... repair DNA breaks or apopto- sis is ...of DNA double-strand breaks, homologous recombination and nonhomologous end joining (16, 36, ...of DNA double-strand breaks include H2AX and the ... See full document

... helper virus for productive replication inevitably leads to competition for cellular resources as well as for the helper factors themselves, which often are essential for both AAV and the helper ...the ... See full document

... AAV replication resulted in the identification of a single adenoviral helper protein ...AAV replication than that of ...HP complex, were not identified, despite previous evidence of their biological ... See full document

... helper virus and the virus generally used to produce ...rAAV replication are probably not involved in AAV DNA synthesis ...AAV replication possible through regulation of cellular gene ... See full document

... HSV-1 replication occurs at the level of DNA replication, while only modestly affecting IE and early gene expression, and that the effect is dependent on AAV replication and/or ... See full document

... the herpes simplex virus type 1 (HSV-1) ICP0 protein promotes the reactivation of rep gene expression in HA-16 cells latently infected with wild-type ... See full document

... Herpes simplex virus (HSV) gene expression during the course of in vitro infection has been well characterized (5, 6, 20, ...a complex consisting of the viral tegument protein virion protein ... See full document

... the replication efficiencies of an 146-bp oriS-con- taining plasmid and a 190-bp oriL-containing ...more complex than the promoter regulatory regions of early ...the DNA polymerase transcript is only ... See full document

... of DNA molecules ranging in size up to 9 ...of DNA chains largely per- ...unit-length DNA molecules ...circle replication of the circular plasmid, is the same as that previously observed with ... See full document

... AAV2 multiplication was similarly efficient in HSV-1 coinfections of HeLa cells, whereas lower yields were obtained in HEp-2 and primary human embryonic kidney cells.. HSV-1 also support[r] ... See full document

... viral DNA replication complex like a replisome, a small factory of replication proteins (4, ...a complex would require the viral DNA polymerase but not necessarily polymerase ... See full document

... AN1 virus infection (43) and demonstrate that UL98 can restore viral repli- cation to near wild-type levels in the absence of UL12 and ...UL98 virus dem- onstrated a slight delay in viral ... See full document