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[PDF] Top 20 Detection and identification of human Plasmodium species with real time quantitative nucleic acid sequence based amplification

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Detection and identification of human Plasmodium species with real time quantitative nucleic acid sequence based amplification

Detection and identification of human Plasmodium species with real time quantitative nucleic acid sequence based amplification

... specific real-time Quantitative Nucleic Acid Sequence Based Amplification (real-time QT-NASBA) assays, based on the small- subunit 18S rRNA ... See full document

6

Quantitative Detection of Hepatitis B Virus DNA by Real Time Nucleic Acid Sequence Based Amplification with Molecular Beacon Detection

Quantitative Detection of Hepatitis B Virus DNA by Real Time Nucleic Acid Sequence Based Amplification with Molecular Beacon Detection

... target amplification resulting in RNA ...for nucleic acid detection. The homogeneous real-time detection allows a closed-tube format of the assay, avoiding any ... See full document

10

Detection of Aspergillus fumigatus in a Rat Model of Invasive Pulmonary Aspergillosis by Real Time Nucleic Acid Sequence Based Amplification

Detection of Aspergillus fumigatus in a Rat Model of Invasive Pulmonary Aspergillosis by Real Time Nucleic Acid Sequence Based Amplification

... Legionella species and Vibrio cholerae (7, 16); and pathogens from food and environmental samples (3, ...Aspergillus species (14, 36); nevertheless, the good diagnostic value of NASBA was demonstrated by ... See full document

6

Comparison between Quantitative Nucleic Acid Sequence Based Amplification, Real Time Reverse Transcriptase PCR, and Real Time PCR for Quantification of Leishmania Parasites

Comparison between Quantitative Nucleic Acid Sequence Based Amplification, Real Time Reverse Transcriptase PCR, and Real Time PCR for Quantification of Leishmania Parasites

... RNA amplification methods for detection of Leishmania parasites have advantages regarding sensitivity and potential quantitative characteristics in comparison with conventional diagnostic methods but ... See full document

6

Real Time Nucleic Acid Sequence Based Amplification Assay for Rapid Detection and Quantification of agr Functionality in Clinical Staphylococcus aureus Isolates

Real Time Nucleic Acid Sequence Based Amplification Assay for Rapid Detection and Quantification of agr Functionality in Clinical Staphylococcus aureus Isolates

... a quantitative agr functionality assay that simultaneously measures RNAIII and gyrB mRNA in a single- tube reaction using a real-time duplex NASBA ...RNA amplification is achieved in a ... See full document

5

Nucleic Acid Sequence Based Amplification with Oligochromatography for Detection of Trypanosoma brucei in Clinical Samples

Nucleic Acid Sequence Based Amplification with Oligochromatography for Detection of Trypanosoma brucei in Clinical Samples

... as real-time nucleic acid sequence-based amplification (NASBA) and PCR, have been developed to detect Trypanosoma brucei parasites in blood for the diagnosis of ... See full document

6

Nucleic Acid Sequence Based Amplification (Nasba)-Prospects And Applications

Nucleic Acid Sequence Based Amplification (Nasba)-Prospects And Applications

... a quantitative NASBA test for HIV-1 viral load determination based on ECL detection is commercially available (NASBA HIV-1 RNA QT, ...95% detection rate of 176 copies/mL, using 1 mL of EDTA, ... See full document

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Rapid Real Time Nucleic Acid Sequence Based Amplification Molecular Beacon Platform To Detect Fungal and Bacterial Bloodstream Infections

Rapid Real Time Nucleic Acid Sequence Based Amplification Molecular Beacon Platform To Detect Fungal and Bacterial Bloodstream Infections

... timely identification using conventional test methods, yet the NASBA assays identified both a gram- positive and a gram-negative organism in the blood culture at the time the sample was flagged as ...gen ... See full document

12

Real Time PCR for Detection and Identification of Plasmodium spp

Real Time PCR for Detection and Identification of Plasmodium spp

... accurate detection of malaria parasites in blood is needed to institute proper ...a real-time PCR assay to detect and distinguish four Plasmodium ...cause human disease by using a ... See full document

6

Nucleic Acid Sequence Based Amplification Assays for Rapid Detection of West Nile and St  Louis Encephalitis Viruses

Nucleic Acid Sequence Based Amplification Assays for Rapid Detection of West Nile and St Louis Encephalitis Viruses

... in human clinical specimens is also significant, given the nonspecificity of the IgM ELISA and the time required to serologically confirm WN virus infection by the PRNT ...from human specimens in ... See full document

8

Detection of Human Cytomegalovirus pp67 Late Gene Transcripts in Cerebrospinal Fluid of Human Immunodeficiency Virus Type 1 Infected Patients by Nucleic Acid Sequence Based Amplification

Detection of Human Cytomegalovirus pp67 Late Gene Transcripts in Cerebrospinal Fluid of Human Immunodeficiency Virus Type 1 Infected Patients by Nucleic Acid Sequence Based Amplification

... the detection of la- tently infected cells or extracellular DNA fragments in patients without active HCMV disease at the time of testing who did not progress to disease for upward of 3 years ...that ... See full document

6

Nucleic Acid Amplification Assays for Detection of La Crosse Virus RNA

Nucleic Acid Amplification Assays for Detection of La Crosse Virus RNA

... of nucleic acid sequence-based amplification (NASBA) and quantitative real-time reverse transcription (RT)-PCR assays for the detection of La Crosse (LAC) ... See full document

5

Nucleic Acid Sequence Based Amplification Assay for Human Papillomavirus mRNA Detection and Typing: Evidence for DNA Amplification

Nucleic Acid Sequence Based Amplification Assay for Human Papillomavirus mRNA Detection and Typing: Evidence for DNA Amplification

... 2010 Human papillomavirus (HPV) E6/E7 mRNA has been proposed as a more specific marker for cervical dysplasia and cancer than HPV ...of nucleic acid sequence-based amplification ... See full document

6

Sequence Based Optimization of a Quantitative Real Time PCR Assay for Detection of Plasmodium ovale and Plasmodium malariae

Sequence Based Optimization of a Quantitative Real Time PCR Assay for Detection of Plasmodium ovale and Plasmodium malariae

... lecular detection using PCR is becoming increasingly ...our detection assays for Plasmodium malariae and Plasmodium ovale by sequencing the 18S rRNA region and developing a new primer and ... See full document

6

Detection of Dengue Viral RNA Using a Nucleic Acid Sequence Based Amplification Assay

Detection of Dengue Viral RNA Using a Nucleic Acid Sequence Based Amplification Assay

... METHODS Human serum or plasma samples. Anonymous human serum samples from patients with acute dengue infections were received from existing collections in Indonesia, Peru, and ...normal human serum ... See full document

5

Detection of Mycoplasma pneumoniae in Spiked Clinical Samples by Nucleic Acid Sequence Based Amplification

Detection of Mycoplasma pneumoniae in Spiked Clinical Samples by Nucleic Acid Sequence Based Amplification

... Isothermal nucleic acid sequence-based amplification (NASBA) was applied to the detection of Mycoplasma ...the detection of inhibitors was ...of detection of ... See full document

7

Prevalence of Plasmodium spp  in malaria asymptomatic African migrants assessed by nucleic acid sequence based amplification

Prevalence of Plasmodium spp in malaria asymptomatic African migrants assessed by nucleic acid sequence based amplification

... Discussion In many countries, the increase in imported malaria cases from different countries is due to the increasing number of people traveling to and from endemic areas, without taking appropriate prophylaxis or ... See full document

7

Comparison and Evaluation of Real Time PCR, Real Time Nucleic Acid Sequence Based Amplification, Conventional PCR, and Serology for Diagnosis of Mycoplasma pneumoniae

Comparison and Evaluation of Real Time PCR, Real Time Nucleic Acid Sequence Based Amplification, Conventional PCR, and Serology for Diagnosis of Mycoplasma pneumoniae

... duplex real-time PCR assay for detection of ...control real-time PCR is described. In addition, real-time nucleic acid sequence-based ... See full document

6

Development of Real Time Multiplex Nucleic Acid Sequence Based Amplification for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp  in Respiratory Specimens

Development of Real Time Multiplex Nucleic Acid Sequence Based Amplification for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp in Respiratory Specimens

... From the 51 specimens from 33 M pneumoniae PCR-positive patients, 40 were PCR positive upon reextraction-amplifica- tion. Within this group 15 specimens were negative in multi- plex as well as in mono NASBA reactions, ... See full document

7

Construction Strategy for an Internal Amplification Control for Real Time Diagnostic Assays Using Nucleic Acid Sequence Based Amplification: Development and Clinical Application

Construction Strategy for an Internal Amplification Control for Real Time Diagnostic Assays Using Nucleic Acid Sequence Based Amplification: Development and Clinical Application

... this detection system is that no post-NASBA step is needed, which reduces the risks of cross ...internal amplification control (IAC) (1, 9, 10). An IAC is a nontarget nucleic acid ... See full document

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