[PDF] Top 20 Development of a Novel Genus Specific Real Time PCR Assay for Detection and Differentiation of Bartonella Species and Genotypes
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Development of a Novel Genus Specific Real Time PCR Assay for Detection and Differentiation of Bartonella Species and Genotypes
... The genus Bartonella includes numerous species with varied host associations, including several that infect ...of Bartonella species while maintaining genus specificity has been ... See full document
5
Development of a Real Time, TaqMan Reverse Transcription PCR Assay for Detection and Differentiation of Lyssavirus Genotypes 1, 5, and 6
... the detection of rabies and rabies-related ...in real time. The TaqMan assay is rapid, sensitive, and specific and allows for the genotyping of unknown isolates concomitant with the ... See full document
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Development of Novel Real Time PCR Assays for Detection and Differentiation of Eleven Medically Important Aspergillus and Candida Species in Clinical Specimens
... Candida-specific real-time PCR assays have been based on species-specific TaqMan probes or hybrid- ization probes (8, 16, 26, 37, 38, 43, ...the specific detection ... See full document
9
Novel Real Time PCR Assay for Simultaneous Detection and Differentiation of Clostridium chauvoei and Clostridium septicum in Clostridial Myonecrosis
... the real-time PCR system, permitting differentiation of ...and specific- ...probe, specific for both species, was incorporated into the assay to enhance its ... See full document
6
Development and Evaluation of a Genus Specific, Probe Based, Internal Process Controlled Real Time PCR Assay for Sensitive and Specific Detection of Blastocystis spp
... The genus comprises at least 13 subtypes (STs) (which likely represent separate species), 9 of which have been found in ...nuclease real-time PCR (TaqMan PCR) including an ... See full document
5
Development and validation of a real time PCR assay for the detection of clinical acanthamoebae
... and specific The 18S rRNA gene is commonly used for developing primers for both detection and sequencing of protists because of its high conservation across the target genus, and its regions of ... See full document
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Evaluation of Novel Broad Range Real Time PCR Assay for Rapid Detection of Human Pathogenic Fungi in Various Clinical Specimens
... related species of Aspergillus and Penicillium are not able to be discriminated by the 28S rDNA sequence, and sequencing analysis initially ascertains only the genus of the causative ...the genus and ... See full document
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Evaluation of FRET real time PCR assay for rapid detection and differentiation of Plasmodium species in returning travellers and migrants
... A detection limit of 0.5 P. falci- parum per PCR volume was ...per PCR volume to ...per PCR volume. Amplification efficiency of real-time PCR reaction varied between 87 to ... See full document
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Novel Multiplex Real Time PCR Diagnostic Assay for Identification and Differentiation of Mycobacterium tuberculosis, Mycobacterium canettii, and Mycobacterium tuberculosis Complex Strains
... the differentiation of mem- bers of the MTC to the species level is not routinely per- ...available real-time PCR kits for the diagnosis of TB generally identify the MTC but not ... See full document
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A novel real time PCR assay for specific detection of Brucella melitensis
... classical species ( ...five novel species ( ...The species of the genus Brucella can be distin- guished on the basis of phenotype, genotype and preferred ... See full document
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Semiquantitative Species Specific Detection of Bartonella henselae and Bartonella quintana by PCR Enzyme Immunoassay
... of PCR products by dot blot hybridization. Hybridization of the PCR products was performed by the protocol described by Anderson et ...for PCR-EIA. In short, 10 l of each PCR product was ... See full document
5
One Step, Multiplex, Real Time PCR Assay with Molecular Beacon Probes for Simultaneous Detection, Differentiation, and Quantification of Human T Cell Leukemia Virus Types 1, 2, and 3
... immunological assay can detect acute infection with ...rapid detection of these newly discovered viruses. PCR is a good choice for detecting HTLVs but might be less sensitive for low proviral ...is ... See full document
7
Real Time PCR Assay for Detection and Quantification of Hepatitis B Virus Genotypes A to G
... The detection and quantification of hepatitis B virus (HBV) DNA play an important role in diagnosing and monitoring HBV infection as well as assessing therapeutic ...HBV genotypes and the enormous range of ... See full document
9
A Real Time Multiplex PCR Assay for Detection of Elizabethkingia Species and Differentiation between Elizabethkingia anophelis and E meningoseptica
... this genus are phenotypically very similar and historically were separated into five groups ...the genus was reassessed taxonomically ...the genus and among other organizational changes proposed the ... See full document
11
Development of nested polymerase chain reaction detection of Mycosphaerella spp and its application to the study of leaf disease in Eucalyptus plantations
... related species groups from plantation Eucalyptus spp., were highly specific for their respective targets ...The specific primer pairs verified the taxa of 3 to 15 isolates of each of the target ... See full document
13
Multiplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis.
... Real-time PCR of clinical ...multiplex real-time PCR in ...duplicate real-time PCR was calculated to give a final ... See full document
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Development of multiplex real-time PCR assays for identification of members of the Anopheles funestus species group
... three species an assay based on HRM was ...sive real-time PCR machine ...MCA assay, subsequently per- formed less well in the blind genotyping ... See full document
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Development of a Quantitative Real Time PCR Assay for Detection of Mycoplasma genitalium
... The sequences of the gap amplicons of the M-strains were identical to type strain G37. Fifty-seven of the 78 LightCycler- positive clinical samples had the same melting point, indicating an identical probe region. Only ... See full document
8
Diagnosis of Bartonella Endocarditis by a Real Time Nested PCR Assay Using Serum
... OSN-PCR assay that was performed in a different room. In this assay, the two primer pairs had different hybridization temper- atures and the reaction tubes were not opened during the entire ... See full document
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Evaluation of the Novel Helicobacter pylori ClariRes Real Time PCR Assay for Detection and Clarithromycin Susceptibility Testing of H pylori in Stool Specimens from Symptomatic Children
... DNA detection, helped to improve the evaluation of the pa- tient’s H. pylori infection status (12, 17). Most serological tests are not appropriate for pediatric patients due to their low sensitivities for those ... See full document
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