[PDF] Top 20 Development of a PCR Assay for Rapid Detection of Enterococci
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Development of a PCR Assay for Rapid Detection of Enterococci
... results. PCR has provided a means for the culture-independent detection of enterococci in a variety of clinical specimens and is capable of yielding results in just a few ...all PCR-based ... See full document
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Development of a Real Time Fluorescence PCR Assay for Rapid Detection of the Diphtheria Toxin Gene
... culture detection. Consequently, molecular detection of the diphtheria toxin gene, tox, is often the only means by which a laboratory con- firmation of diphtheria can be ...standard PCR assay ... See full document
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Development and Validation of a Real Time PCR Assay for Rapid Detection of Candida auris from Surveillance Samples
... worldwide. Rapid identification of ...real-time PCR assay targeting the internal transcribed spacer 2 (ITS2) region of the ribosomal ...The assay was highly specific, repro- ducible, and ... See full document
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Development of a Rapid PCR Assay Specific forStaphylococcus saprophyticus and Application to Direct Detection from Urine Samples
... coli, enterococci, and streptococci, which are also frequently encountered in urine ...developed PCR assays for the spe- cific detection of other staphylococcal species as well as asso- ciated ... See full document
5
Rapid Detection of Shiga Toxin Producing Bacteria in Feces by Multiplex PCR with Molecular Beacons on the Smart Cycler
... the development of an outbreak and to enable the implementation of control measures to prevent more ...the detection of ...toxin detection are available, but they still require an incubation period ... See full document
5
Rapid Preclinical Detection of Sheeppox Virus by a Real Time PCR Assay
... real-time assay on nasal swabs was 100%, with a specificity of ...real-time assay on day 3, 1 to 3 days prior to the onset of clinical ...real-time PCR. The VI results were con- firmed by ... See full document
5
Development of a Multiplex PCR Assay for Rapid Molecular Serotyping of Haemophilus parasuis
... typing assay. The assay was designed using a wide variety of genes from within the capsule loci, including an aminotransferase, gly- cosyltransferases, O-antigen flippase, and genes with unknown function; ... See full document
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Detection of vancomycin resistant enterococci in fecal samples by PCR
... the PCR assay for detecting vanA-containing enterococcal strains both with purified organisms and with stool ...the detection of the vanC ...the detection of vanB-containing enterococci ... See full document
6
The development of a GeXP based multiplex reverse transcription PCR assay for simultaneous detection of sixteen human respiratory virus types/subtypes
... of PCR, amplification was carried out predominantly by a single pair of universal primers, of which only the for- ward universal primer was labeled ...the assay were function- ing correctly. Only 2 μl of ... See full document
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Development of a loop-mediated isothermal amplification assay for rapid detection of Yersinia enterocolitica via targeting a conserved locus
... (LAMP) assay is a technique that amplifies DNA under isothermal ...this assay, a set of four specific primers are used that anneal to six distinct regions within the target se- ...LAMP assay is not ... See full document
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Development of a direct PCR assay for detection of the diphtheria toxin gene
... the PCR. When boiling for 20 min was used, more than 150,000 CFU/PCR mixture was needed to visualize the ...direct PCR for ...our PCR, but it was still less effec- tive than the QIAamp Blood ... See full document
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Comparison of Methods for Detection of Vaccinia Virus in Patient Specimens
... vial assay for culture of vaccinia is useful for isolation of vaccinia virus from patients with com- plications due to smallpox ...vial assay or directly on smears from lesions to increase ...Real-time ... See full document
5
Molecular Dipstick Test for Diagnosis of Sleeping Sickness
... on detection of parasites in either blood or lymph by ...the development and the first-phase evaluation of a simple and rapid test (HAT-PCR-OC [human African ... See full document
6
Development of a Multiplex PCR Assay for Detection and Genogrouping of Neisseria meningitidis
... high-fidelity PCR was carried out under the following conditions: dena- turation step at 95°C for 15 s, annealing step at 62°C for 30 s, and extension step at 68°C for 10 min for 32 ...The PCR products were ... See full document
6
A diagnostic PCR assay for the detection of an Australian epidemic strain of Pseudomonas aeruginosa
... enable rapid identification of CF individuals that harbour the AES-I strain so that appropriate segregation measures can be employed during hospital and clinic vis- ...and PCR to iden- tify 2 genetic loci ... See full document
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Development of nested polymerase chain reaction detection of Mycosphaerella spp and its application to the study of leaf disease in Eucalyptus plantations
... Molecular detection of plant pathogens is used in many agri- cultural spheres to assist decision support ...Immunological detection of Mycosphaerella bras- sicicola from spore traps has been developed to ... See full document
13
Early Detection of Acute Rhinovirus Infections by a Rapid Reverse Transcription PCR Assay
... the detection of RV ...this assay for the treating physicians, who increasingly used the results for therapeutic decisions during the period of prospec- tive investigation (personal ... See full document
5
Combined PCR oligonucleotide ligation assay for rapid detection of Salmonella serovars
... successful PCR-based procedure for detecting infectious agents in clinical samples is the pres- ence of PCR-inhibitory substances in the sample (10, ...dilute PCR-inhibitory substances and (ii) ... See full document
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Evaluation of Multiplex Tandem Real Time PCR for Detection of Cryptosporidium spp , Dientamoeba fragilis, Entamoeba histolytica, and Giardia intestinalis in Clinical Stool Samples
... first development and evaluation of a multiplex tandem PCR (MT-PCR) assay for the detection and identification of 4 common pathogenic protozoan parasites, Cryptospo- ridium ... See full document
6
Evaluation of Novel Broad Range Real Time PCR Assay for Rapid Detection of Human Pathogenic Fungi in Various Clinical Specimens
... second PCR using a primer/ probe set specific for ITS 1 ...real-time PCR screening based on the ITS 1 and ITS 2 region is prob- lematic due to sequence variability and the absence of a ge- neric probe ... See full document
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