[PDF] Top 20 Development and Validation of an In House Database for Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Based Yeast Identification Using a Fast Protein Extraction Procedure
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Development and Validation of an In House Database for Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Based Yeast Identification Using a Fast Protein Extraction Procedure
... UCSC yeast database might not be exhaustive, we plan to continuously update this reference library with more represen- tative isolates by exploiting the intrinsic expandability of the Bruker Biotyper ...the ... See full document
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Comparison of the Bruker Biotyper and Vitek MS Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Mycobacteria Using Simplified Protein Extraction Protocols
... The combined UW/Bruker and SARAMIS databases reliably differentiated M. tuberculosis from NTM species with their respec- tive extraction procedures. As has been previously reported (18, 19, 21), M. abscessus and ... See full document
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Advances in Identification of Clinical Yeast Isolates by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... MS identification is based on obtaining and comparing the unique protein profile of an isolate to a reference library using spectral pattern ...differentiated using biochemical tests ... See full document
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Evaluation of a Simple Protein Extraction Method for Species Identification of Clinically Relevant Staphylococci by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... atrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has been used to identify various microorganisms in clinical ... See full document
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Identification of Medically Relevant Species of Arthroconidial Yeasts by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... another database that contains a large number of reference spectra ( ⬎ 600 MSPs) generated from different fungal strains, which were obtained from the CBS-KNAW yeast collection and validated previously with ... See full document
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Prospective Evaluation of a Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System in a Hospital Clinical Microbiology Laboratory for Identification of Bacteria and Yeasts: a Bench by Bench Study for Assessing the Impact on Time to Identification and Cost Effectiveness
... MS identification protocol (MALDI ...performed using the Bruker Microflex instrument, Biotyper software ...developed based on the manufacturer’s suggestions and findings from other recent studies ... See full document
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Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Rapid Identification of Tick Vectors
... rapid identification of tick vectors. This method was used for the first time to identify ticks removed from ...the time required for molecular methods, and the completion of this assay does not ... See full document
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Interlaboratory Comparison of Sample Preparation Methods, Database Expansions, and Cutoff Values for Identification of Yeasts by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Using a Yeast Test Panel
... study using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) to determine the identification of ... See full document
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Identification of Blood Culture Isolates Directly from Positive Blood Cultures by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and a Commercial Extraction System: Analysis of Performance, Cost, and Turnaround Time
... others using in-house extraction systems for blood culture analysis which have reported correct identification rates ranging from 67 to 100%, with higher success rates for Gram-negative ... See full document
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Creation of an In House Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Corynebacterineae Database Overcomes Difficulties in Identification of Nocardia farcinica Clinical Isolates
... the database, two kinds of solid media were selected according to the results ob- tained for the genus (Table ...tion procedure, i.e., the use of zirconia/silica beads for extraction with formic ... See full document
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Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry for Fast and Reliable Identification of Clinical Yeast Isolates
... by using the standard ...the database (main spectra), and the results were listed in a ranking ...the database was used for species ...reference database in the course of the study, resulting ... See full document
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Evaluation of the Andromas Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Aerobically Growing Gram Positive Bacilli
... Rapid identification of GPRs should have an important clinical impact on the diagnosis of GPR-associated infectious ...rapid identification of colonies that cannot be distinguished from those of other ... See full document
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Evaluation of the Vitek MS Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Clinically Relevant Filamentous Fungi
... Accurate identification of this organism is important due to its decreased susceptibility to many antifungals, including some azoles, amphotericin B, and micafun- gin ... See full document
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Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Based Method for Discrimination between Molecular Types of Cryptococcus neoformans and Cryptococcus gattii
... T wo pathogenic basidiomycetous yeasts, Cryptococcus neofor- mans and Cryptococcus gattii, are known to cause meningoen- cephalitis in immunocompromised and apparently immunocom- petent human hosts, respectively. ... See full document
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Mass determination of major plasma proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
... values based on techniques such as compositional analysis, electrophoresis, and ultracentrifugation generally showed fairly good agreement for proteins lacking ...glyco- protein, antithrombin III, α 2 ... See full document
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Comparison of analytical profile index, microscan walkaway 40 plus and bruker maldi biotyper systems for identification of enteric pathogens in Kenya
... TOF spectrometry are available for identification and antimicrobial susceptibility profiling of primary ...TOF mass spectrometry that is mainly used for ... See full document
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Comparison of the Microflex LT and Vitek MS Systems for Routine Identification of Bacteria by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... nitrogen laser. The spectra were re- corded in the linear mode in a mass range of 2 to 20 kDa and subsequently analyzed using MALDI Biotyper Automation Control and Biotyper ...the time, the ... See full document
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Fatal Nocardia farcinica Bacteremia Diagnosed by Matrix-Assisted Laser Desorption-Ionization Time of Flight Mass Spectrometry in a Patient with Myelodysplastic Syndrome Treated with Corticosteroids
... and time-consuming processes ...spp. identification from different clinical specimens [22, ...Recently, Matrix-Assisted Laser Desorption-Ionization Time of ... See full document
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Microbial Typing by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Do We Need Guidance for Data Interpretation?
... three protein peaks as specific for 197 of 224 (88%) USA300 isolates ...of mass spectra for a pair of isogenic MRSA and methicillin-sus- ceptible ... See full document
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Biomedical Mass Spectrometry in Today's and Tomorrow's Clinical Microbiology Laboratories
... the fast and precise assessment of the mass of molecules in a range of 100 Da to 100 ...so-called matrix (low- molecular-weight organic acids, usually including an aromatic ...short laser ... See full document
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