[PDF] Top 20 Differential dependence of herpes simplex virus immediate-early gene expression on de novo-infected cell protein synthesis.
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Differential dependence of herpes simplex virus immediate-early gene expression on de novo-infected cell protein synthesis.
... Addition of the protein synthesis inhibitor anisomycin to cells coincident with infection resulted in maximal rates of transcription and accumulation of functional ICPO mRNA, while 0.5 h[r] ... See full document
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Herpes Simplex Virus 1 Immediate-Early and Early Gene Expression during Reactivation from Latency under Conditions That Prevent Infectious Virus Production
... gene expression. This result is similar to those observed with cell culture, in which immediate-early and early gene expression were not reduced by inhibition of ... See full document
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Persistence and Expression of the Herpes Simplex Virus Genome in the Absence of Immediate-Early Proteins
... the expression of specific sets of IE ...cells infected with the multiple IE ...viral gene expression to that of the IE ...in infected Vero cells, d109 does not express ICP4, ICP27, ... See full document
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The herpes simplex virus immediate-early protein ICP0 affects transcription from the viral genome and infected-cell survival in the absence of ICP4 and ICP27.
... another early gene that differs from ICP6 in that its expression is more markedly reduced in the absence of ICP4 (or ICP4 and ...IE gene and, therefore, is transcribed in the absence of viral ... See full document
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Cellular SNF2H Chromatin Remodeling Factor Promotes Herpes Simplex Virus 1 Immediate Early Gene Expression and Replication
... were infected with HSV-1 at an MOI of ...The cell pellets were frozen at ⫺ 80°C, thawed on ice, and lysed with SDS lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris [pH ... See full document
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Reactivation of Expression from Quiescent Herpes Simplex Virus Type 1 Genomes in the Absence of Immediate-Early Protein ICP0
... Gene expression during coinfection and ...gene expression. HFFF2 cells, incubated at 38.5°C for 8 days, were infected with a fixed amount ...per cell) of in1374, together with ... See full document
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Repression of gene expression upon infection of cells with herpes simplex virus type 1 mutants impaired for immediate-early protein synthesis.
... lytically infected cells provides a back- ground of gene expression which, at the experimental level, masks events in cells containing quiescent ...IE gene expression even further have ... See full document
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Role of Herpes Simplex Virus 1 Immediate Early Protein ICP22 in Viral Nuclear Egress
... IE protein, ICP22, a 420-residue protein encoded by the Us1 gene, conserved in the subfamily Alphaherpesvirinae of the family Her- ...in cell cultures in a cell type-dependent manner ... See full document
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Transcriptional Coactivators Are Not Required for Herpes Simplex Virus Type 1 Immediate-Early Gene Expression In Vitro
... were infected with RP5 or RP5R strains of HSV-1 at an MOI of 0.025 PFU/cell and 5 PFU/cell, which corresponded to about 8 to 10 viral genomes per cell for each ...to cell culture plates ... See full document
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Prolonged gene expression and cell survival after infection by a herpes simplex virus mutant defective in the immediate-early genes encoding ICP4, ICP27, and ICP22.
... Very early in infection, herpes simplex virus (HSV) expresses four immediate-early (IE) regulatory proteins, ICP4, ICP0, ICP22, and ...cells infected with virus ... See full document
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The Role of Herpes Simplex Virus ICP27 in the Regulation of UL24 Gene Expression by Differential Polyadenylation
... temporal expression of UL24 tran- scripts was regulated by differential polyadenylation ...at early times in infection and then de- crease, whereas long transcripts ...were infected ... See full document
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Role of Immediate Early Protein ICP27 in the Differential Sensitivity of Herpes Simplex Viruses 1 and 2 to Leptomycin B
... blocks herpes simplex virus 1 (HSV-1) replication in Vero cells and that certain mutations in viral immediate early protein ICP27 can confer LMB ...ICP27 gene determines ... See full document
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Regulation of herpes simplex virus poly (A) site usage and the action of immediate-early protein IE63 in the early-late switch.
... viral protein expression from an IE63-deficient mutant virus to examine the role of IE63 in ...vivo. Expression of the true late genes US11, UL38, UL44, and UL45 required the IE63 ...of ... See full document
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The PK Domain of the Large Subunit of Herpes Simplex Virus Type 2 Ribonucleotide Reductase (ICP10) Is Required for Immediate-Early Gene Expression and Virus Growth
... IE gene expression by PK-defective ...wild-type virus, ICP10 PK downregulates a protein which inhibits early onset of IE gene ...latently infected with HSV-1, but it was ... See full document
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Differential Role of Sp100 Isoforms in Interferon-Mediated Repression of Herpes Simplex Virus Type 1 Immediate-Early Protein Expression
... of expression and/or the combination of these IFN-upregulated isoforms is necessary for long-term cell ...Input virus had been titrated to result in infection of approximately 90% of cells as assayed ... See full document
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Multimerization of ICP0, a herpes simplex virus immediate-early protein.
... To determine whether any of the transactivation-deficient IE-0 mutants that we isolated displayed a dominant negative phenotype, Vero cells were cotransfected with equimolar amounts of a[r] ... See full document
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Isolation and characterization of deletion mutants of herpes simplex virus type 1 in the gene encoding immediate-early regulatory protein ICP4.
... To determine more specifically whether the rates of synthesis of ICPO, ICP27, and ICP6 in d120- and d202-infected cells differ from that in tsB32-infected cells and to examine the possib[r] ... See full document
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Immediate-early gene expression is sufficient for induction of natural killer cell-mediated lysis of herpes simplex virus type 1-infected fibroblasts.
... HOWELL,' LISA PETTERA,1 SHAHRAM TEHRANI,1 AND CARLOS LOPEZ2 Department of Laboratory Medicine and Pathology, University of Medicine and Dentistry of New Jersey-New Jersey Medical School,[r] ... See full document
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Control of VP16 Translation by the Herpes Simplex Virus Type 1 Immediate-Early Protein ICP27
... cells infected with viruses lacking ...VP16 gene expres- sion, we undertook a series of experiments that carefully com- pared the levels of VP16 protein and mRNA in cells infected with HSV ... See full document
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Herpes Simplex Virus Immediate-Early Protein ICP0 Is Targeted by SIAH-1 for Proteasomal Degradation
... of herpes sim- plex virus 1 and 2, ICP0(1) and ICP0(2), respectively, formed a complex with SIAH-1 by coimmunoprecipitation ...with expression vectors encoding GFP-tagged ICP0 together with HA-tagged ... See full document
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