[PDF] Top 20 DNA Binding Properties of Simian Virus 40 Temperature-Sensitive A Proteins

... The binding regions were defined by the ability of A protein to protect simian virus 40 DNA from digestion with limited footprint assay or excess fragment assay amounts of DNase I.. At l[r] ... See full document

... Small DNA tumor viruses such as simian virus 40 (SV40), polyomavirus, or papillomavirus have become important model systems for the study of molecular events involved in DNA ...viral ... See full document

... of simian virus 40 (SV40) makes it ideal for studying the complex processes of virion ...5,243-bp DNA genome, which is enclosed within an icosahedral capsid made up of three virally encoded ... See full document

... This, and the fact that mutants of the A group induce host DNA synthesis in CV-1 cells although they do not replicate viral DNA at 40 C 5, 18 suggested either that an early function othe[r] ... See full document

... We showed previously that simian cellular DNA-binding factors the Band A factors bind to sequences within the SV40 late promoter which are important for transcriptional activation in the[r] ... See full document

... single-stranded DNA binding and the ability to cause structure distortion of origin DNA, suggesting that single- stranded DNA binding is also needed for structural ...origin ... See full document

... Interestingly, the cross-linking of N260 generated a species that migrated faster than the intact monomer (not cross- linked) and which was formed even at low protein concentra- tions (Fig. 3C; lanes 2 to 5 form the left ... See full document

... Mutant TAgs purified from recombinant virus-infected human 293 cells were shown to be unable to mediate the replication of viral DNA in vitro, while wild-type TAg that had been similarly[r] ... See full document

... Presence of singile copies or tandem repeats of SV40 sequences iiztegrated in the cell were generated at initial multiplicities of infecgenome of transforrm ed CHL cells tion of from 5 t[r] ... See full document

... Premixing the labeled reticulocyte cell-free products with the same amount of infected-cell extract normally used in a DNA binding experiment, representing a great excess over that synth[r] ... See full document

... For the initial screening of mutants data in Table 1 the 24 well plates of Linbro Chemical Co., New 116 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Temperature-sens[r] ... See full document

... Double mutants of A:B, A:C, and A:BC complementation groups, like their A parent, were defective in viral DNA replication, in the induction of host DNA synthesis, and in the transformati[r] ... See full document

... To determine whether mutant SV40tsTNG-1 also induced cellular DNA synthesis, 5-day-old cultures of primary mouse kidney cells 3.3 X 106 cells per culture were infected at input multiplic[r] ... See full document

... SCHWEDES,' AND PETER TEGTMEYER1* Department of Microbiology, State University of New York, Stony Brook, New York 11794-8621,' and Department of Microbiology, Specialized Cancer Research [r] ... See full document

... tsA1499-infected cells were kept for 96 h at 32 or 41°C, radiolabeled with [32P]phosphate, extracted, and analyzed for both T-p53 complexes and free T antigen by sequential immunoprecipi[r] ... See full document

... 22 showed that dephosphorylation by alkaline phosphatase increases the affinity of T antigen to the SV40 binding site II and, furthermore, that the activity of dephosphorylated T antigen[r] ... See full document

... In contrast, studies carried out with lytic T-antigen subclasses separated by zone velocity sedimentation or phosphocellulose chromatography showed that newly synthesized 5 to 7S T antig[r] ... See full document

... Nucleotide inhibition of origin DNA binding is observed in three different DNA-binding assays with immunopurified COS1 T antigen, biochemically purified lytic T antigen, and crude COSi c[r] ... See full document

... To obtain direct evidence that this region of the T antigen molecule is not only conformationally important but is, in fact, sufficient to act as the binding site with specificity for th[r] ... See full document

... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Initiation[r] ... See full document