[PDF] Top 20 DNA Macroarray for Identification and Typing of Staphylococcus aureus Isolates
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DNA Macroarray for Identification and Typing of Staphylococcus aureus Isolates
... is Staphylococcus au- reus, by virtue of its frequent and highly versatile pathogenicity in humans and ...animals. Isolates belonging to this species are responsible for suppurative infections and syndromes ... See full document
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Validation of Virulence and Epidemiology DNA Microarray for Identification and Characterization of Staphylococcus aureus Isolates
... 64 isolates analyzed while 12 gene targets were absent in all isolates examined (see Appendix S2 in the supple- mental ...the identification genes (11%), genes encoding adhesins (25%), proteases ... See full document
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Comparison of Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis as Tools for Typing Staphylococcus aureus Isolates in a Microepidemiological Setting
... Bacterial isolates, isolate identification, and antibiotic susceptibility ...The isolates were identified as S. aureus on the basis of positive catalase, coagulase, and DNase ...S. ... See full document
7
Genetic Variation among Staphylococcus aureus Strains from Bovine Milk and Their Relevance to Methicillin Resistant Isolates from Humans
... the isolates were analyzed by PFGE, which was performed as described by Ichiyama et ...Chromosomal DNA included in the thinly sliced agarose gel was digested with 10 U of SmaI or XhoI for 18 h and then ... See full document
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Comparison of DNA Sequencing of the Protein A Gene Polymorphic Region with Other Molecular Typing Techniques for Typing Two Epidemiologically Diverse Collections of Methicillin ResistantStaphylococcus aureus
... developed typing approach for methicillin-resistant Staphylococcus aureus (MRSA) based on the DNA sequencing of the protein A gene polymorphic region (spaA typing) with a combination of ... See full document
9
Novel Multiple Locus Variable Number Tandem Repeat Analysis Method for Rapid Molecular Typing of Human Staphylococcus aureus
... the typing of Staphylococcus aureus isolates ...used typing schemes include multilocus sequence typing (MLST) and spa typing ...repetitive DNA loci (5, 8, 9, 12, ... See full document
5
Molecular Typing and Distribution of Staphylococcus aureus Isolates in Eastern Canadian Dairy Herds
... chromosomal DNA, using pulsed field gel electrophoresis (PFGE) was performed to type and estimate genetic relationships among 288 Staphylococcus aureus isolates recovered from 58 Eastern ... See full document
7
External Quality Assessment of Molecular Typing of Staphylococcus aureus Isolates by a Network of Laboratories
... of isolates with indistin- guishable, clonally related, and unrelated PFGE patterns were included in each ...ⱕ6 DNA fragment differences and were designated by a capital letter ...ⱕ3 DNA fragment ... See full document
9
Methods for Identification of Staphylococcus aureus Isolates in Cases of Bovine Mastitis
... The isolates with unsatisfactory identification scores were additionally identi- fied by rrs sequencing as ...Mich.). Identification was achieved by comparison of edited sequences against GenBank ... See full document
5
Good Performance of the SpectraCellRA System for Typing of Methicillin Resistant Staphylococcus aureus Isolates
... different typing methods are in use in diagnostic laboratories, with pulsed-field gel electrophoresis (PFGE) of SmaI macrorestriction analysis of genomic DNA still being considered the gold standard because ... See full document
5
Restriction enzyme analysis of plasmid DNA and bacteriophage typing of paired Staphylococcus aureus blood culture isolates
... Still, when we placed isolates in a limited number of phage groups to minimize the problem of single strong-reaction differences as well as to use weak reactions for typing purposes, onl[r] ... See full document
6
Determination of Iplex Massarray as a Method for Genetic Typing and Characterization of Staphylococcus Aureus Isolates In Kenya
... of DNA sequence based techniques, PFGE was regarded as a simple and versatile technique for establishing physical and optical genetic ...S. aureus outbreaks (Frickmann et ...in typing certain STs ... See full document
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spa Typing of Staphylococcus aureus as a Frontline Tool in Epidemiological Typing
... spa typing in combination with BURP (based upon repeat pattern) grouping analysis as a frontline tool in the epidemiological typing of Staphylococcus aureus, based on a random collection of ... See full document
8
Clinical Evaluation of the DiversiLab Microbial Typing System Using Repetitive Sequence Based PCR for Characterization of Staphylococcus aureus Strains
... fungal isolates and to identify some fungi to the species level ...after DNA extraction, the rep-PCR method uses primers that target and bind to multiple noncoding, repetitive sequences (generally 30 to 500 ... See full document
6
Comparison of Staphylococcus aureus Isolates from Bovine and Human Skin, Milking Equipment, and Bovine Milk by Phage Typing, Pulsed Field Gel Electrophoresis, and Binary Typing
... phage typing (18, 19), ribotyping (18, 23), multilocus enzyme electrophoresis (7, 15), random amplified polymorphic DNA typing (7), PFGE (3, 30, 43), coagulase gene typing (20), and binary ... See full document
9
Comparison of traditional and molecular methods of typing isolates of Staphylococcus aureus
... Phage typing, plasmid DNA restriction analysis, and antibiogram analysis, the techniques most readily available to clinical laboratories, identified 23 to 26 of 29 outbreak-related isola[r] ... See full document
9
High prevalence of USA300 among clinical isolates of methicillin-resistant Staphylococcus aureus on St. Kitts and Nevis, West Indies
... ST8 isolates, a rooted maximum likelihood tree was generated from core genome SNPs, performed as previously described by Strauß et ...S. aureus TCH1516, a USA300 ST8 strain (GenBank accession ...the ... See full document
7
Comparative Study of Inducible and Constitutive Clindamycin Resistance among Methicillin Resistant Staphylococcus Aureus Isolates.
... Environmental condition like P H , temperature and salt concentration also decides the expression of methicillin resistance 39,40 . In R.Skov et al 2006 suggest that incubation temperature influences zone diameter and ... See full document
148
Prevalence of Genes Encoding Pyrogenic Toxin Superantigens and Exfoliative Toxins among Strains of Staphylococcus aureus Isolated from Blood and Nasal Specimens
... S. aureus isolates derived from clinical ...S. aureus isolates with the genetic information to produce toxins irre- spective of their ...S. aureus isolates by the use of two ... See full document
6
The clinical and molecular epidemiology of Staphylococcus aureus infections in Fiji
... MSSA isolates, 105 underwent ...MSSA isolates (Figure 1) with the most common be- ing CC5, 7, 14, 75 and ...Two isolates produced a previously undescribed SNP profile and full MLST revealed that they ... See full document
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