[PDF] Top 20 DNA Manipulation in Microfluidic Devices.

... in this system. The light source was replaced by a 50 mW, 355 nm UV pulse laser (CryLa). It generates 10 ns pulses at a repetition rate up to 1000 Hz. Each pulse is 50 µJ in energy. A pair of polarizers was placed in the ... See full document

... The manipulation of magnetic beads on-chip was recently reviewed by Gijs ...fractionation devices and similar methodologies could therefore be useful to researchers developing chip-based magnetic separation ... See full document

... include DNA, ion channels, nuclear receptors and ...discovered. DNA sequencing projects have revealed novel genes with characteristics similar to G protein-coupled ... See full document

... PCR devices, droplets have been used to generate DNA libraries, including genomic libraries and cDNA ...of DNA leads to artifactual ...template DNA and high numbers of PCR cycles [72, ... See full document

... reactions (PCR) have been used with selective primers to amplify and identify DNA and RNA molecules within large and expensive thermal cyclers. Recently, it has become possible to build compact heating and cooling ... See full document

... In Chapter 3, we used optical epifluorescence microscopy and three-dimensional finite element simulations to investigate the binding of biotin-coated QDots to an array of streptavidin-coated agarose beads immobilized in ... See full document

... as DNA, which is hydrophilic ...foreign DNA to within a cell whilst preserving its ...of DNA for genetic therapy has the potential to cure diseases through the incorporation of extra or missing ... See full document

... In recent years, dynamic acoustic approaches, where the radiation force changes with time, have gained increasing interest for particle manipulation. These methods allow to reconfigure the acous- tic field within ... See full document

... There is a growing technology-driven interest in using external influences to move or shape small quantities of liquids, referred to as microfluidic actuation. The use of electrical, rather than mechanical, forces ... See full document

... a DNA solution 共of concentration c= 4c * , with c * being the overlap concentration兲 in flows through 2:1 abrupt con- ...both DNA and XG are rigid rod-like ... See full document

... cell-free DNA and exosomes) from whole blood could complement a routine physical exam as a non-invasive way to screen for the potential presence of cancer disease at a very early stage of ... See full document

... screening: microfluidic devices, im- aging technologies, and imaging analysis ...various microfluidic devices and imaging modalities, have more precise manipulations and extract deeper in- ... See full document

... There is a growing technology-driven interest in using external influences to move or shape small quantities of liquids, referred to as microfluidic actuation. The use of electrical, rather than mechanical, forces ... See full document

... flow [Fuerstman 2007, Hibara 2005]. Another case is the mixture of different liquid flows in the system [Sudarsan 2006]. The liquids filled into the system from different channels can be mixed in the system, flowing ... See full document

... Due to high surface to volume ratio, fluid/surface interaction will significantly influence the droplet dynamics in microfluidic devices. Figure 9 shows the flow patterns at various Ca for three different ... See full document

... The Program Counter executes the program in the addition of one instruction, and the value of the program counter can be altered at any moment, which causes a jump to a new memory location. The main Motherboard of the ... See full document

... the microfluidic device for particular applications; hard tubing will ensure robustness, pressure stability (no tube deformation) prior droplet generation while soft and flexible tubes will provide adequate ... See full document

... Our confocal data also showed that there was no obvious im- pairment or blockage of viral capsids resulting in a localized accu- mulation in the cytoplasm of the cell body, in the axon hillock or proximal axons (Fig. ... See full document

... In Chapter 3.3, leveraging the analytical hardware described in Chapter 3.2, a new analytical approach has been successfully innovated and implemented for the characterization of phenols and alkyl phenols in organic ... See full document

... System. In brief, primers were designed for all mutations as listed in Table S1 in Additional file 1 and Illumina- specific adapters were attached to the 5′ ends in round of PCR. PCR was performed using the FastStart ... See full document