[PDF] Top 20 Effects of Mutations within the Herpes Simplex Virus Type 1 DNA Encapsidation Signal on Packaging Efficiency

... a packaging signal, and we also showed that it functions with an efficiency similar to that of larger fragments containing one or two copies of the complete a sequence, including the highly ... See full document

... of DNA it packaged, the difference was much smaller for molecules up to about 100 kbp in size than for those closer in size to full-length genomes ...of DNA into the ...during DNA ... See full document

... measure packaging, Southern blotting and TCA precipitation, may indicate the fates of different populations of viral DNA ...and packaging are mechanistically ...tious virus particles at an ... See full document

... UL11 packaging. To compare the relative levels of packaging for wt and mutant derivatives of UL11, a transfection/infection-based assay was ...of DNA per 100-mm plate for each con- struct) using ... See full document

... for packaging of mature, linear ...on encapsidation-deficient mu- tants showed that cleavage of the genome occurs at the time of encapsidation and that mutations of the capsid proteins that ... See full document

... inhibit packaging of amplicon vector ...of virus replication (ori-S) and one packaging signal (a) (5, 19, 20, 32, ...reduced packaging efficiency of the ...aging signal a ... See full document

... of herpes simplex virus type 1 (HSV-1), a new class of thiourea inhibitors was ...varicella-zoster virus were inhibited to a lesser ...viral DNA into progeny genome ... See full document

... for packaging (including the portal protein) are mainly structural components of the ...for DNA packaging are replication-generated concatemers, and the DNA is cleaved into genome-size ... See full document

... infectious virus. In the absence of DNA packaging, the internal scaffolding proteins are still cleaved but remain trapped inside the angularized capsid, generating a dead-end product, the B ... See full document

... resulting mutations in the three regions affect ICP0’s subcellular and subnuclear localization (Phos 3), ND10-dis- rupting activity in a subset of cells (Phos 2 and 3), and trans- activating activity (Phos ... See full document

... To demonstrate that the two UL28 mutants contain the deletion/insertion mutations at the appropriate position in the viral genome, we isolated total-cell DNA from Vero cells infected wit[r] ... See full document

... viral DNA accumu- lates as concatemers in the nuclei of infected ...This DNA is then cleaved into monomeric lengths and inserted into pre- formed capsids through a unique portal vertex (reviewed in ... See full document

... null virus dlUL33 in transient assays and also failed to support cleavage and packaging of replicated amplicon DNA ...with efficiency similar to that of the wt UL33 in these assays, while the ... See full document

... herpesvirus DNA. By introducing single copies of test packaging signals into an HSV-1 genome lacking the normal resident copies, we have overcome one of the major drawbacks of the previously used ... See full document

... Cluster 1 is located in the apical region of the protein lo- cated on the same face as the view shown in ...cluster 1, traverses laterally across the entire molecule and abuts disordered loop ...cluster ... See full document

... for DNA cleavage and ...of DNA-containing C capsids on the capsid ...after DNA is packaged; (ii) to trigger nuclear egress of the capsid once a stable DNA-containing capsid is formed; (iii) to ... See full document

... (NC-5), 1:2,000; anti-VP19C (NC-2), 1:2,000 (NC-2 and NC-5 antibodies were gifts from Gary Cohen and Roselyn Eisenberg) ...diluted 1:5,000 in PBST with 5% milk and allowed to react with immunoblots ... See full document

... the packaging factor encoded by UL28 ...the packaging machinery could also serve as a proofreading appa- ratus which normally prevents unpackaged capsids from pro- ceeding to later stages of assembly ... See full document

... FGF4 signal peptide, with an RRKK tetramer attached to its amino terminus to improve solubility, inhibits both the entry and cell-to-cell spreading of HSV-1 and that the inhibition depends largely or ... See full document

... rescued DNA with ...the mutations in a rescued plas- mid, a control transfection-superinfection was performed using the pol mutant, HP66, which is unable to ...rescued DNA with ... See full document