[PDF] Top 20 Epstein-Barr Virus (EBV) Nuclear Antigen 1 Colocalizes with Cellular Replication Foci in the Absence of EBV Plasmids

... EBNA-1 colocalizes with the microscopically detected cellular replication foci or replication factories (8, 10, 19, 20, 27, 31), in the absence of EBV ...labeled ... See full document

... of EBV contains 20 high-affinity sites for EBNA1, which binds each of these sites as a dimer ...oriP plasmids to cellular chromosomes [15-19]; an association that facilitates the plasmids to ... See full document

... vent EBV immortalization of primary B cells in vitro ...of EBV-driven B-cell growth ...(Fig. 1), the elevated levels of bfl-1 mRNA that re- main in ...the absence of either EBV ... See full document

... Epstein-Barr virus (EBV) is a human gamma herpesvirus that has been implicated in several lymphoid and epithelial cell malignancies (reviewed in reference ...60). EBV establishes a ... See full document

... the nuclear localization signal of EBNA1 (amino acids 379 to 386 [2]), which was not altered in any of our EBNA1 mu- ...test plasmids to be in the nucleus, and therefore, a mutation that inhibits plasmid ... See full document

... the EBV genome, suggesting that the majority of cellular sites are bound directly through the EBNA1 DNA binding ...different virus copy numbers ...the EBV genome but that cell type variations ... See full document

... and replication assays in C666-1 cells. (A) C666-1 cells were transfected with pCAN (negative control), OriP, or OriPE plasmid as indicated, harvested after 3 to 42 cell doublings (6 to 84 days), and ... See full document

... performed nuclear run-on assays of Qp-directed transcription in the presence and absence of ...the virus replication cycle (23, 30, 33, ...of EBV-negative Louckes BL cells, ... See full document

... the replication or partitioning of oriP plasmids (data not ...oriP plasmids correlated with an association between EBNA1 and metaphase chromosomes ...(aa 1 to 450) is replaced by human ... See full document

... the absence of a plasmid containing oriP. Indeed, EBNA-1 binds not only to cellular chromatin and chromosomes but also to oriP during the cell cycle ...and cellular chro- matin binding involve ... See full document

... for 1 h at ...using 1/50 of the ChIP DNA and Platinum SYBR green qPCR SuperMix-UDG (Invitrogen) in a Rotorgene qPCR system (Corbett ...using 1/2,500 of each sample, and values obtained for ChIP ... See full document

... the absence of DNA replication when Rta was expressed in Raji cells ...(30). EBV DNA is incapable of undergoing DNA replication in the Raji cell line due to an inactivating mutation in BALF2, ... See full document

... (5). Virus from this line was passaged to B cells from the common marmoset, Callithrix jacchus, and two LCLs (M81 and M82) were generated ...(6). EBV virions from M81 were then used to generate LCLs from ... See full document

... both EBV proteins with ...one cellular protein is regulated by phosphoryla- tion (39); we have previously demonstrated that EBNA-3C itself is a phosphoprotein, though it is not known whether this ... See full document

... of replication at DS in the same way that they prevent the loss of plasmids from nuclei, by tethering plasmids to cellular ...tether plasmids containing the KSHV terminal repeats (to ... See full document

... of EBV persistence remains a matter of ...in EBV-positive humans ...to EBV and a similar repertoire of latent genes which appear, from recent studies on the EBNA2 and LMP homologues, to have ... See full document

... for virus replication and are Met129 ...Met129-positive EBV isolate selected by in vitro pressures and is consistent with the hypothesis that Met129 serves a function during productive infection but ... See full document

... To identify the cis-regulatory sequences of the HIV-1 LTR target for EBNA2 activation, we performed cell transfection experiments in which the wild-type or mutant HIV-1 LTR plasmids carr[r] ... See full document

... 677-686 0022-538X/91/020677-10$02.00/0 Copyright C 1991, American Society for Microbiology Single-Stranded Structures Are Present within Plasmids Containing the Epstein-Barr Virus Latent[r] ... See full document

... early EBV promoters (BHLF1 and BHRF1) continued to be active (or in the case of the BHLF1 promoter, actually increased) in the presence of the BMRF1 gene product, even though BZLF1 binding to these promoters was ... See full document