[PDF] Top 20 Establishing reference genes for use in real-time quantitative PCR analysis of early equine embryos

... (2011) Establishing reference genes for use in real-time quantitative PCR analysis of early ... See full document

... erences genes in qRT-PCR studies, normalization procedures have received little attention for this spe- cies, with many studies continuing to use a single reference gene and most failing to ... See full document

... before use for normalization of quantitative mRNA assay before and after a therapeutic ...on equine gene expression have used GAPDH as an internal RG for normalization ... See full document

... and use various strategies to induce cell apop- tosis, cell transformation, cell death or other dysfunctions by shifting host gene expression on different ...“housekeeping” genes is probably unstable in ... See full document

... these reference genes have constant level of expression in different tissues and under different treatments and has no inter-individual variability ...ideal reference gene for normalisa- tion in ... See full document

... Six genes that presented high levels of stability in the microarray were tested in RT-qPCR, as well as two other genes commonly used in RT-qPCR data ...the use of GeNorm, Norm- Finder and ΔCq ... See full document

... from real-time PCR analysis, accurate normalization of gene expression against a reference gene is ...The reference gene should have stable expression independent of the ... See full document

... candidate reference genes chosen were ana- lyzed by three different statistical models because we assumed that a comparison of different algorithms enables a better evaluation of the most reliable ... See full document

... the use of two unique rDNA-based primer pairs ...candidate reference genes for normalization of expression levels in ...of reference genes as well as their optimal number by using the ... See full document

... to use a natural source of target mRNA, thereby avoiding the need to make a separate plasmid for each gene of interest while simultaneously correcting for differences in reverse tran- scription ...the genes ... See full document

... Because only a small quantity of RNA can be extracted from one embryo, an RNA pre-amplification step was included in the protocol. Ribo-SPIA, the isothermal mes- senger RNA amplification method used, has been shown to be ... See full document

... and PCR process. The use of HKGs as refer- ence genes to normalize gene expression is a strategy to minimize the variations of ...good reference gene should meet three ... See full document

... stable reference gene (V 4/5 = ...a reference gene by BestKeeper analysis we would suggest to use the first five reference genes Rps18, Canx, Sdha, Pgk1 and Actg1 for calculating ... See full document

... Background: Real-time quantitative polymerase chain reaction (RT-qPCR) analysis is a low cost and sensitive tech- nique that is widely used to measure levels of gene ...appropriate ... See full document

... Several PCR-based techniques (16, 18, 24) have been devel- oped for the diagnosis of toxoplasmosis using various clinical specimens, including amniotic fluid (3, 11), blood (1, 13, 17), cerebrospinal fluid (27), ... See full document

... performed PCR assays, we decided to exploit the recently developed real-time ...linear quantitative detection range of 5 logs with a calculated detection limit of one to three spirochetes per ... See full document

... The most striking result of our examination of clinical biopsy samples was that many that were negative for H. pylori by culture contained large amounts of H. pylori DNA when ana- lyzed by Q-PCR. A possible ... See full document

... Reducing chemical pesticide use is a desirable goal for agriculture and forestry. One disease control method, the application of fungi or bacteria as microbial antagonists of plant pathogens, offers prospects of ... See full document

... common reference gene in many experiments (Glare et ...the reference genes due to higher stability in ...as reference gene due to high variation in different tissues at adult ...a ... See full document

... candidate reference genes evaluated in the present study were included in the research of Park et ...the genes with relatively moderate stable ...stable genes were only average performers in ... See full document