[PDF] Top 20 Evaluation of a Novel PCR Based Assay for Detection and Identification of Chlamydia trachomatis Serovars in Cervical Specimens
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Evaluation of a Novel PCR Based Assay for Detection and Identification of Chlamydia trachomatis Serovars in Cervical Specimens
... collection. Cervical specimens were collected from women participating in the enrolment visit of a community-based double- blind randomized clinical trial investigating the efficacy of an HPV type ... See full document
6
Evaluation of the NucliSens Basic Kit for Detection ofChlamydia trachomatis and Neisseria gonorrhoeaein Genital Tract Specimens Using Nucleic Acid Sequence Based Amplification of 16S rRNA
... from assay to assay. For PCR, the optimal annealing condi- tions, including temperature and primer concentration, must by determined to prevent nonspecific priming ...the detection level by ... See full document
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Original Article Novel real-time polymerase chain reaction assay for quantitative detection of Chlamydia trachomatis in vaginal and cervical secretions
... are based on various target genes, such as the 16S ribosomal RNA gene, ompA, and a cryptic plasmid ...[12-15]. PCR assay based on the cryptic plasmid is regarded sensitive for detection ... See full document
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Combination of PCR Targeting the VD2 of omp1 and Reverse Line Blot Analysis for Typing of Urogenital Chlamydia trachomatis Serovars in Cervical Scrape Specimens
... new PCR-based typing assay, directed to the VD2 region of the omp1 gene, for the detection and typing of urogenital Chlamydia trachomatis ...VD2 PCR- reverse line blot ... See full document
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Detection of Neisseria gonorrhoeae and Chlamydia trachomatis in genitourinary specimens from men and women by a coamplification PCR assay
... C. trachomatis infection is frequently based on isolation in tissue ...gonorrhea, detection of chlamydia usually involves an invasive ...C. trachomatis, and a single dose of a quinolone ... See full document
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Evaluation of Conventional and Real Time PCR Assays Using Two Targets for Confirmation of Results of the COBAS AMPLICOR Chlamydia trachomatis/Neisseria gonorrhoeae Test for Detection of Neisseria gonorrhoeae in Clinical Samples
... cppB PCR-EIA only, and 19 of these were derived from 17 female occupational sex workers all employed in the same ...and cervical swab samples were ob- tained from 35 women for culturing and nucleic acid ... See full document
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Use of PCR and Reverse Line Blot Hybridization Assay for Rapid Simultaneous Detection and Serovar Identification of Chlamydia trachomatis
... All specimens were tested for C. trachomatis using the COBAS AMPLICOR (Amplicor; Roche Diagnostics Australia ...C. trachomatis and one set targeting the cryptic plasmid were designed or modified from ... See full document
6
RNA amplification by nucleic acid sequence based amplification with an internal standard enables reliable detection of Chlamydia trachomatis in cervical scrapings and urine samples
... the detection of Chlamydia trachomatis infection was ...a detection limit of 1 inclusion-forming unit (IFU), while the 16S rRNA appeared to be the most sensitive RNA target for amplification ... See full document
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COMPARISON OF PCR BASED ASSAY AND CELL CULTURE IN DETECTION OF UROGENITA L CHLAMYDIA TRACHOMATIS INFECTION AND DETERMINATION OF THE INHIBITORY RATE O F SPECIMENS UNDER STUDY
... infection, detection of infected individuals is a ma jor challenge to the preventive health care ...swab specimens collected from symptomatic and asymptomatic male and females has been the approach to ... See full document
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Multicenter Evaluation of the BDProbeTec ET System for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Urine Specimens, Female Endocervical Swabs, and Male Urethral Swabs
... System Chlamydia trachomatis and Neisseria gonorrhoeae Amplified DNA Assays (BD Biosciences, Sparks, ...4,131 specimens were compared to those from culture and the LCx nucleic acid amplification test ... See full document
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Multicenter Evaluation of the Fully Automated COBAS AMPLICOR PCR Test for Detection of Chlamydia trachomatis in Urogenital Specimens
... swab specimens from women because two initially inhibitory specimens were found to be true positive upon ...clinical specimens contain substances that inhibit PCR, the ligase chain reaction, ... See full document
7
Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction
... A rapid and sensitive polymerase chain reaction PCR-based assay for detection of Chlamydia trachomatis in cervical specimens is described.. This assay consists of i sample preparation wh[r] ... See full document
5
Evaluation of the Vidas Chlamydia test to detect and verify Chlamydia trachomatis in urogenital specimens
... Vidas Chlamydia test (CHL) is an automated enzyme-linked immunofluorescence assay for the detec- tion of Chlamydia ...CHL assay is widely used in Europe, but ...the identification of ... See full document
5
Evaluation of the Abbott RealTime CT/NG Assay in Comparison to the Roche Cobas Amplicor CT/NG Assay
... CT/NG assay used for clinical testing in our hospital system and the Abbott RealTime CT/NG ...assay. Specimens were tested with the Roche system and either tested concurrently using the Abbott system ... See full document
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Detection of Chlamydia trachomatis by the Gen Probe AMPLIFIED Chlamydia Trachomatis Assay (AMP CT) in Urine Specimens from Men and Women and Endocervical Specimens from Women
... Using a larger sample size, preliminary studies at the Cleveland Clinic also revealed a lower sensitivity for the Abbott ID NOW, with a false negative rate of 14.8%. However, the samples were all stored in viral ... See full document
19
IS1533 based PCR assay for identification of Leptospira interrogans sensu lato serovars
... A PCR-based assay was developed for typing ...lato serovars. The assay is designed to exploit the presence of many copies of the leptospiral insertion sequence IS1533 and IS1533-like ... See full document
6
Determination of Chlamydia trachomatisPrevalence in an Asymptomatic Screening Population: Performances of the LCx and COBAS Amplicor Tests with Urine Specimens
... urine specimens for the detection of Chlamydia trachomatis in an asymptomatic screening ...Urine specimens (n ⴝ 2,906; 1,138 specimens from men and 1,717 specimens from ... See full document
5
Neisseria Species Identification Assay for the Confirmation of Neisseria gonorrhoeae Positive Results of the COBAS Amplicor PCR
... rRNA PCR assays (18; data not shown). PCR conditions consisted of an initial denaturation at 95°C for 10 min followed by amplification for 50 cycles of 10 s at 95°C, 15 s at 55°C, and 7 s at 72°C with ... See full document
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Development and Validation of a Rotor Gene Real Time PCR Assay for Detection, Identification, and Quantification of Chlamydia trachomatis in a Single Reaction
... C. trachomatis infection (3, 5, 6, 7, 13, 17), and a repeat of the same assay with the initial extract or DNA newly extracted from the same sample did not improve the accuracy of diagnosis (5, 6, ...new ... See full document
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Prevalence of Chlamydia infection among women visiting a gynaecology outpatient department: evaluation of an in-house PCR assay for detection of Chlamydia trachomatis
... pain (32.0%), infertility (4.3%) and other STD related symptoms. In our study overall prevalence of STD among females with STI related symptoms was 21.2% (593/2800). STI was more common among patients in the age group of ... See full document
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