[PDF] Top 20 Evaluation of rapid radiometric method for drug susceptibility testing of Mycobacterium tuberculosis

... A total of 106 isolates of Mycobacterium tuberculosis were tested for drug susceptibility by the conventional 7Hll plate method and by a new rapid radiometric method using special 7H12 l[r] ... See full document

... national tuberculosis program that follows the World Health Organization rec- ommendations for diagnosis and directly observed therapy short course ...for tuberculosis, and the sputum sample-based diagnosis ... See full document

... Radiometric antimicrobial susceptibility tests, if performed by using pure culture recovered from a conventional solid medium, required approximately 5 days with 98.6% agreement with the[r] ... See full document

... proportion method were reevaluated, the result varied in 8 out of 24 tests by Etest, and in 9 out of 24 tests by the proportion ...the drug susceptibility testing by both methods, but this was ... See full document

... (24). Rapid, accurate diagnosis and the determination of drug sus- ceptibility are crucial to optimize treatment and prevent trans- ...used method for Mycobacterium tu- berculosis drug ... See full document

... multi drug resistant (MDR) M. tuberculosis from smear positive/negative pulmonary specimens and ...M. tuberculosis cultures after evaluation by the Foundation for Innovative new diagnostics ... See full document

... persistent Mycobacterium tuberculosis than any other conventional anti-TB drug ...reliable testing of the sus- ceptibility of M. tuberculosis to PZA in vitro is challenging since PZA ... See full document

... sputum testing by ...antimicrobial drug stock solutions were prepared at the fol- lowing concentrations: INH, ...four drug stock solutions was added to a well containing sputum-medium ...the ... See full document

... of susceptibility testing methods have been devel- oped for ...proportion method. In this method, drug-free and drug-con- taining media are inoculated with known dilutions of the ... See full document

... high-throughput testing. Here, we present a further extension of our SMB TB drug resistance detection assay system, adding assays that enable detection of resistance to AMK and ...typic ... See full document

... excellent method able to provide rapid and reliable results of susceptibility to first- line ...the radiometric system and are being evaluated for MTC suscep- tibility ...for ... See full document

... multidrug-resistant tuberculosis (MDR- TB) caused by Mycobacterium tuberculosis and, recently, exten- sively drug-resistant tuberculosis caused by an MDR strain that is also resistant ... See full document

... proportion method agreed with the results generated by the MB/BACT TABLE 1. Drug susceptibility results for clinical strains of ...M. tuberculosis as determined by MB/BACT and BACTEC 460TB ... See full document

... of drug suscep- tibility testing (DST) for PZA, the results of phenotypic re- sistance are not always reliable ...reliable method to perform PZA-DST [7, 8] and is currently the only available ... See full document

... of tuberculosis has caused considerable effort to be focused on the development of rapid methods for determining the susceptibility of Mycobacterium tuberculosis to antimycobacterial ... See full document

... BACTEC and conventional methods of antimicrobial susceptibility testing were compared with the use of artificial mixtures of 1% resistant and 99% susceptible Mycobacterium tuberculosis s[r] ... See full document

... highly drug-resistant strains ...determine susceptibility to SM, KM, AK, and CM, we first obtained susceptibility data for SM and KM using the absolute concentration method on LJ slants (lab ... See full document

... M. tuberculosis cells in spiked sputum samples with ␾ 2 ...M. tuberculosis cells per ml, processed with NALC and NaOH, and incubated for 12 h with ␾ 2 ...M. tuberculosis cells were ...M. ... See full document

... tion method by the commercially available AccuProbe M. tuberculosis complex test (Gen Probe, Inc., San Diego, Calif.) (6, 8, 13) according to the instructions of the manufacturer. The test consists of the ... See full document

... direct drug susceptibility ...the susceptibility set was removed after scanning, and an inventory report was ...printed. Susceptibility results for both INH and RIF (in the first option) were ... See full document