[PDF] Top 20 Functional Analyses of the EBNA1 Origin DNA Binding Protein of Epstein-Barr Virus

... the EBNA1 functions require the binding of EBNA1 dimers to 18-bp palindromic recognition sites (3, ...The EBNA1 amino acids responsible for DNA binding and dimer- ization ... See full document

... Rta binding to the EBV genome during early replication, our results yielded important insights regarding late gene promoter regula- ...an origin of lytic rep- FIG 7 Rta interacts with late gene promoters ... See full document

... of DNA. Cross-linked EBNA1 produced a laddering of complexes with the dominant species at 32 kDa, consistent with EBNA1 dimers ...WT EBNA1 and most of the mutants formed cross-linked dimers of ... See full document

... simplex virus type 1 (HSV-1) ICP4 (IE175) protein consisting of 38% homology (20% identical and 18% similar residues) over a region of 178 codons ...reflects functional homol- ogy between the two ... See full document

... by EBNA1 are involved in the oxidative stress response, including the antioxidants SOD1 and ...The EBNA1-induced increase in both of these proteins was validated by Western blotting, although only SOD1 was ... See full document

... stranded DNA binding protein BALF2, the helicase BBLF4, the primase BSLF1, and the primase-associated factor ...simplex virus (HSV) or of Kaposi’s sarcoma- associated herpesvirus can replicate ... See full document

... EBNA-1 binding sites within DS have been detected by an electrophoretic mobility shift assay (2) and by DNase I foot- printing (10, 11, ...lowest-affinity binding site (10, ...EBNA-1 binding sites in ... See full document

... by EBNA1 was let-7a. let-7a induction in response to EBNA1 expres- sion was verified in multiple EBV-negative carcinoma cell lines, while EBNA1 silencing in multiple EBV-positive carcinoma cell lines ... See full document

... UL8 protein on the basis of positional similarity of the two genes in their respective genomes, a small stretch of amino acid similarity with UL8 of HSV and gene 51 of vari- cella-zoster virus within a ... See full document

... (Qp), EBNA1 binds to two 18 bp sequences immediately downstream of the transcriptional start site, and functions as an inhi- bitor of transcription initiation and mRNA accumulation ...[9]. EBNA1 binds ... See full document

... fide binding partner of Zta, in vivo protein association assays were carried out in two cell systems containing EBV that were permissive to lytic re- ... See full document

... Cell lines expressing either EBNA1 solid bars; a deletion mutant expressing the DNA binding domain, nuclear localization sequence, and acidic carboxy terminus EBDB, hatched bar; or neith[r] ... See full document

... coli onC, the SV40 origin, and yeast origins, since the dyad symmetry region contains an easily unwound sequence as well as binding sites for an essential replication protein EBNA1.. How[r] ... See full document

... 7. Functional analysis of mutant oriLyt plasmids in the transient replication ...simian virus 40 origin of replication which replace two different regions of oriLyt, including the single Sp1 site at ... See full document

... The EBNA1 325–376 region has been shown to mediate an interaction with one cellular protein that is associated with mitotic chromosomes, termed EBP2 (EBNA1 binding protein 2) ...A ... See full document

... EBNA1 protein bound to a plasmid containing FR, the herpes simplex virus type 1 thymidine kinase promoter, and the firefly luciferase gene was electroporated into cells.. Downloaded from[r] ... See full document

... above, EBNA1 is expressed in all latency programs except latency 0, and is also present in all EBV-positive tumors and represents the only latent protein in some of these tumors, such as in latency I ... See full document

... Unlike the interaction of bEBNA1 with onP, in which one element FR is bound prior to interaction Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest a bEBNAl proteins 1.4 pm[r] ... See full document

... on EBNA1 transcriptional activation or on its ability to attach to cellular chromosomes (a prereq- uisite for ...the functional role of methylation is complicated by the fact that EBNA1 is a very ... See full document

... gammaherpesvirus, Epstein-Barr virus (EBV), is the etiological agent underlying diseases such as infectious mono- nucleosis, Burkitt’s lymphoma, Hodgkin’s disease, nasopharyn- geal carcinoma, ... See full document