[PDF] Top 20 Functional Analysis of the Triplex Proteins (VP19C and VP23) of Herpes Simplex Virus Type 1

... The triplex of herpesvirus capsids is a unique structural element. In herpes simplex virus type 1 (HSV-1), one molecule of VP19C and two of VP23 form a three-pronged ... See full document

... shell proteins (VP5, VP19C, and VP23) are expressed in the absence of functional scaffolding proteins, they form structures that have the appearance of partial and incomplete shells in negatively ... See full document

... the triplex structure. The triplex, which is unique to herpesviruses, is a complex of three proteins, two molecules of VP23 which interact with a single molecule of ...identify functional ... See full document

... pseudorabies virus (PrV) encodes a 534-amino-acid protein with a molecular mass of 57 kDa (7, 11, ...the herpes- virus life cycle and it has indeed been shown that pUL25 homologs play an essential ... See full document

... indicated virus at an MOI of ...for 1 h with 7% normal goat serum and 7% BSA in TBS (TBS blocking buffer) before incubation for 5 h with either anti-V5 (Invitrogen, Carlsbad, CA) for recognition of gK, ... See full document

... wild type redistributes pUL34 into cytoplasmic puncta that colocalize with a capsid ...TEM analysis led us to hypothesize that VLVs are abnor- mal primary virions mislocalized into cytoplasmic ...VP5-null ... See full document

... solubilized virus prepara- tions by immunoprecipitation first with PAb and then with protein A-Sepharose (25, ...cross-linked proteins was incubated in an equal volume of binding buffer (10 mM Tris [pH ... See full document

... 2428 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The possible identity of the herpes simplex virus type 1 HSV-1 65K 65,000-Mr virion protein which stimulates transc[r] ... See full document

... The results showed that the major capsid polypeptide VP5 and two other capsid proteins, VP19 and VP23, were degraded rapidly beginning as soon as capsids were exposed to the ion plasma..[r] ... See full document

... Wild-type HSV enters CHO–nectin-1 cells via a low-pH-de- pendent endocytic pathway (2). This pathway is thought to be critical for HSV infection of human keratinocytes (1). HSV deter- minants of the ... See full document

... wt virus-infected cells, ICP35 c,d was processed to ICP35 e,f ...lanes 1, 2, and ...wt virus-infected Vero cells (Fig. 4, compare lanes 1 and ...lanes 1, 7, and ... See full document

... Differing behaviors have also been described for the UL36 mutants. Luxton et al. (30) reported that deletion of UL36 from PrV caused a dramatic reduction in capsid egress from nuclei. They suggested that the absence of ... See full document

... of 1/10,000 (A, C, E, G, I, K, M, O, Q, and S) and polyclonal anti-UL30 r113 at a dilution of 1/3,500 (B, D, F, H, J, L, N, P, R, and ...at 1/100 and Cy3-conjugated goat anti-mouse IgG (Amersham) at ... See full document

... phatase 1 (FL-18) and mitogen-activated protein kinase phosphatase (C-19); goat polyclonal antibodies to SmB (N-18), SC35 (Y-16), hnRNP F (N-15), SMN (N-19), the 70-kDa U1 snRNP protein (C-18), and actin (I-19); ... See full document

... nucleotide 1 (14), and at the EcoRI site that occurs in the linker insertion in these ...dues 1 to 163, was joined to the C-terminal portion of mutant S13 from residues 264 to ...residues 1 to ... See full document

... null virus dlUL33 in transient assays and also failed to support cleavage and packaging of replicated amplicon DNA ...UL33 proteins by probing Western blots with antibodies 9E10 (Myc) or R148, respectively ... See full document

... Phenotypic analysis of mlOO shows that i normal amounts of the c and d forms of ICP35 were produced, but they failed to be processed to the cleaved forms, e and f; ii viral DNA replicati[r] ... See full document

... A TATA element is the only consensus sequence element identified in the upstream regulatory region of late viral promoters 18, and deletion studies have indicated that little sequence in[r] ... See full document

... As discussed below, the analysis suggested that overall functional amino acid homology between the portion of the alkaline exonuclease enzymes encoded by the HSV-1 and HSV-2 1.9-kb trans[r] ... See full document

... each virus. The analysis selected a number of genes shown to be more up-regulated with the WT virus than with the ⌬ ...WT virus harnesses the machinery of MEFs to allow normal cellular ... See full document