[PDF] Top 20 Herpes Simplex Virus Type 1 Cleavage and Packaging Proteins UL15 and UL28 Are Associated with B but Not C Capsids during Packaging
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Herpes Simplex Virus Type 1 Cleavage and Packaging Proteins UL15 and UL28 Are Associated with B but Not C Capsids during Packaging
... various cleavage and packaging proteins can be proposed based on the results pre- sented in this paper taken together with other ...that cleavage and packaging occur within replication ... See full document
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Herpes Simplex Virus 1 DNA Packaging Proteins Encoded by UL6, UL15, UL17, UL28, and UL33 Are Located on the External Surface of the Viral Capsid
... all herpes- viruses. Herpes simplex virus (HSV) capsid pentons and hex- ons form spontaneously from five and six molecules of ICP5, respectively; these capsomeres are linked by triplexes ... See full document
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Herpes Simplex Virus DNA Cleavage and Packaging Proteins Associate with the Procapsid prior to Its Maturation
... infected-cell proteins, the cells were pelleted by centrifugation at 1,000 ⫻ g for 5 min, washed once in D-PBS, resuspended in loading buffer (58), probe sonicated, and boiled for 3 min prior to ...and B-, ... See full document
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Proteolytic Cleavage of the Amino Terminus of the UL15 Gene Product of Herpes Simplex Virus Type 1 Is Coupled with Maturation of Viral DNA into Unit-Length Genomes
... resemble B capsids in protein content but contain an internal core of larger diameter and a highly porous external shell ...small-cored B capsid is coupled with cleav- age of the internal shell from ... See full document
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Evidence for Controlled Incorporation of Herpes Simplex Virus Type 1 UL26 Protease into Capsids
... wild-type B capsids but not C capsids (71) (Fig. 8B). The UL28 protein is found predominantly on wild- type B but not C capsids (62, ...the ... See full document
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Herpes Simplex Virus Type 1 DNA-Packaging Protein UL17 Is Required for Efficient Binding of UL25 to Capsids
... Herpes simplex virus type 1 packages its DNA genome into a precursor capsid, referred to as the ...DNA-packaging proteins, UL17, UL25, and UL6, only UL17 and UL6 appear to ... See full document
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Herpes Simplex Virus Type 1 Portal Protein UL6 Interacts with the Putative Terminase Subunits UL15 and UL28
... tant proteins UL6in161, UL6in269, UL6in368, and UL6in378 (Table 1) were constructed by ligating a self-annealing oligonucleotide, 5⬘ CGCGAGATCTCG 3⬘, containing an internal BglII site to linear partials of ... See full document
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The bovine herpesvirus 1 maturational proteinase and scaffold proteins can substitute for the homologous herpes simplex virus type 1 proteins in the formation of hybrid type B capsids.
... deduced proteins encoded by the BHV-1 and HSV-1 UL26 and ...BHV-1-encoded proteins showed con- servation of several functionally important ...proteolytic cleavage sites were also ... See full document
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Linker Insertion Mutations in the Herpes Simplex Virus Type 1 UL28 Gene: Effects on UL28 Interaction with UL15 and UL33 and Identification of a Second-Site Mutation in the UL15 Gene That Suppresses a Lethal UL28 Mutation
... the UL28 homologs from human and animal herpesviruses ...HSV UL28 gene) and HSV-1 UL28 proteins are both sequence-specific DNA binding ...the UL28 protein for protein/DNA ...the ... See full document
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Intracellular Cre-Mediated Deletion of the Unique Packaging Signal Carried by a Herpes Simplex Virus Type 1 Recombinant and Its Relationship to the Cleavage-Packaging Process
... of B-type ...most virus mutated in genes implicated in cleavage packaging, such as UL6, UL15, UL17, UL28, UL32, and UL33 (reviewed in references 13, 26, 29, and ...of ... See full document
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Characterization of ICP6::lacZ insertion mutants of the UL15 gene of herpes simplex virus type 1 reveals the translation of two proteins.
... The herpes simplex virus type 1 (HSV-1) UL15 gene is a spliced gene composed of two exons and is predicted to encode an 81-kDa protein of 735 amino acids ...Two ... See full document
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The Herpes Simplex Virus Type 1 UL17 Gene Encodes Virion Tegument Proteins That Are Required for Cleavage and Packaging of Viral DNA
... h. Virus capsids were isolated and purified from nuclear lysates of infected cells on a 20 to 50% sucrose gradient, essentially as described previously ...7.4], 1 mM ... See full document
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Structural Characterization of the UL25 DNA-Packaging Protein from Herpes Simplex Virus Type 1
... A capsids and DNase I-sensitive unit-length genomes ac- cumulated in the nuclei of cells infected with KUL25NS, sug- gesting that, although complete genomes could be packaged, the viral DNA was only transiently ... See full document
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Packaging of the Virion Host Shutoff (Vhs) Protein of Herpes Simplex Virus: Two Forms of the Vhs Polypeptide Are Associated with Intranuclear B and C Capsids, but Only One Is Associated with Enveloped Virions
... lanes b to ...(lanes b and e) or in the presence of 5 g/ml of actinomycin D (ActD) (lanes c and f) or 50 g/ml of cycloheximide (Cyclo) (lanes d and ... See full document
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Mutational Analysis of the Herpes Simplex Virus Type 1 DNA Packaging Protein UL33
... (AcUL33 virus) or mutated (Acin14 to Acin116) forms of UL33 and with AcUL28Myc, expressing a c-Myc-tagged form of ...Panel c shows that UL28-Myc and UL33 were detected in the starting cell ... See full document
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Packaging-Competent Capsids of a Herpes Simplex Virus Temperature-Sensitive Mutant Have Properties Similar to Those of In Vitro-Assembled Procapsids
... vitro herpes simplex virus (HSV) capsid assembly product which, because of certain parallels between its properties and those of bacteriophage proheads, they have designated the ...mature ... See full document
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The pseudorabies virus UL28 protein enters the nucleus after coexpression with the herpes simplex virus UL15 protein.
... all UL28-null and UL28-temperature- sensitive mutants is a cessation of viral replication at the point of DNA cleavage and encapsidation (1, 6, 14, 17, ...of UL28 is to act in concert ... See full document
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The Herpes Simplex Virus Type 1 Cleavage/Packaging Protein, UL32, Is Involved in Efficient Localization of Capsids to Replication Compartments
... the cleavage and packaging of herpesvirus DNA into preassembled ...mutant virus is unable to cleave and package viral DNA, consistent with the phenotype of a previously isolated temperature-sensitive ... See full document
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Herpes Simplex Virus DNA Cleavage and Packaging: Association of Multiple Forms of UL15-Encoded Proteins with B Capsids Requires at Least the UL6, UL17, and UL28 Genes
... for 1 h in a Beckman SW41 ...and 1 mM EDTA) and pelleted at 20,000 rpm in a SW41 rotor for 2 ...separated proteins were either stained with Coomassie brilliant blue or transferred to nitrocellulose ... See full document
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DNA Cleavage and Packaging Proteins Encoded by Genes UL28, UL15, and UL33 of Herpes Simplex Virus Type 1 Form a Complex in Infected Cells
... a 1-ml volume of buffer consisting of PBS containing 1% Tween 20 ...eluted proteins were separated by electrophoresis on a denaturing polyacrylamide ...Table 1 and detailed below in the ... See full document
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