[PDF] Top 20 Herpes simplex virus type 1 origin-specific binding protein: oriS-binding properties and effects of cellular proteins.
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Herpes simplex virus type 1 origin-specific binding protein: oriS-binding properties and effects of cellular proteins.
... Incubation of infected cell extracts with the lower-affinity site II DNA as a probe resulted in the appearance of two protein-DNA complexes with mobilities identical to those of the A an[r] ... See full document
11
Use of transdominant mutants of the origin-binding protein (UL9) of herpes simplex virus type 1 to define functional domains.
... in protein expression levels, a transient transfection-immunoblot experiment was ...cell protein extracts were resolved by SDS-PAGE, immunoblotted, and analyzed by us- ing the UL9-specific rabbit ... See full document
8
Alphaherpesvirus Proteins Related to Herpes Simplex Virus Type 1 ICP0 Affect Cellular Structures and Proteins
... The herpes simplex virus type 1 (HSV-1) immediate-early protein ICP0 interacts with several cellular proteins and induces the proteasome-dependent ... See full document
12
Interaction between Herpes Simplex Virus Type 1 IE63 Protein and Cellular Protein p32
... The herpes simplex virus type 1 (HSV-1) immediate-early gene IE63 (ICP27), the only HSV-1 regulatory gene with a homologue in every mammalian and avian herpesvirus ... See full document
7
Properties of the novel herpes simplex virus type 1 origin binding protein, OBPC.
... inhibit origin-dependent viral DNA replica- ...by binding to its cognate DNA binding sites at viral origins, OBPC serves to inhibit de novo initiation by competing with OBP for ...other ... See full document
7
Helicase Motif Ia Is Involved in Single-Strand DNA-Binding and Helicase Activities of the Herpes Simplex Virus Type 1 Origin-Binding Protein, UL9
... multifunctional protein essential for herpes simplex virus type 1 (HSV-1) replication in ...and origin-binding activities. It is thought that UL9 binds the ... See full document
12
Origin Binding Protein-Containing Protein-DNA Complex Formation at Herpes Simplex Virus Type 1 oriS: Role in oriS-Dependent DNA Replication
... to oriS site I when nu- clear extracts of Vero or Nd-PC12 cells are used as the source of ...of protein described binding of two HSV-specific complexes (complexes A and B) to oriS site ... See full document
9
Cellular Transcription Factors Enhance Herpes Simplex Virus Type 1 oriS-Dependent DNA Replication
... The herpes simplex virus type 1 (HSV-1) origin of DNA replication, oriS, contains three binding sites for the viral origin binding ... See full document
11
DNA Binding Activity of the Herpes Simplex Virus Type 1 Origin Binding Protein, UL9, Can Be Modulated by Sequences in the N Terminus: Correlation between Transdominance and DNA Binding
... inhibitory effects exhibited by C-terminal UL9 and by wild-type UL9 itself, resulting in 63 and 78 normalized plaques, respec- tively ...UL9 protein in HSV-1 replication is relieved in the ... See full document
10
Phosphorylation of the Herpes Simplex Virus Type 1 Origin Binding Protein
... tor proteins by phosphorylation has been well established (50); however, assessment of the phosphorylation state of OBP has been challenging due to the very low level of OBP expressed in HSV-infected cells ... See full document
10
Kinetics of expression of the gene encoding the 65-kilodalton DNA-binding protein of herpes simplex virus type 1.
... To characterize more thoroughly the kinetics with which the 65KDBP gene is expressed, we have examined not only the accumulation of 6SKDBP mRNA and protein, as compared to other well-cha[r] ... See full document
11
DNA binding and gene regulation by the herpes simplex virus type 1 protein ICP4 and involvement of the TATA element.
... The regions of the herpes simplex virus type 1 immediate early protein Vmw 175 required for site specific DNA binding closely correspond to those involved in transcriptional regulation..[r] ... See full document
11
Herpes Simplex Virus Type 1 DNA-Packaging Protein UL17 Is Required for Efficient Binding of UL25 to Capsids
... UL17-stop virus contains a 16-bp insertion at position 32718 in the HSV-1 strain 17 genome, with stop codons in all three reading frames, truncating the UL17 open reading frame after codon 260 ...with ... See full document
9
Herpes Simplex Virus Type 1 Helicase-Primase: DNA Binding and Consequent Protein Oligomerization and Primase Activation
... fork proteins, the helicase-primase com- plex (UL5-UL8-UL52), and the DNA polymerase (UL30- ...single-stranded binding protein of HSV, has been reported to stimulate the primase and DNA-dependent ... See full document
11
Relationship between TATA-binding protein and herpes simplex virus type 1 ICP4 DNA-binding sites in complex formation and repression of transcription.
... Effect of relative TATA box and ICP4-binding-site position on tripartite complex formation and repression. Plasmids pi4, pi7, pi10, pi14, pi18, pi22, pi27, pi40, pi45, and pi54, which contain the ICP4 promoter ... See full document
8
Purification of the herpes simplex virus type 1 65-kilodalton DNA-binding protein: properties of the protein and evidence of its association with the virus-encoded DNA polymerase.
... 8 Purification of the Herpes Simplex Virus Type 1 65-Kilodalton DNA-Binding Protein: Properties of the Protein and Evidence of Its Association with the Virus-Encoded DNA Polymerase MICHA[r] ... See full document
10
Expression of the varicella-zoster virus origin-binding protein and analysis of its site-specific DNA-binding properties.
... It was previously shown, by using a protein A fusion protein, that the product of gene 51 is a site-specific DNA-binding protein which binds to sequences within the VZV origin Stow et al[r] ... See full document
9
Thermolabile in vivo DNA-binding activity associated with a protein encoded by mutants of herpes simplex virus type 1.
... 3 Thermolabile In Vivo DNA-Binding Activity Associated with a Protein Encoded by Mutants of Herpes Simplex Virus Type 1 Received 30 December 1982/Accepted 17 March 1983 The major DNA-bin[r] ... See full document
11
The 60-Residue C-Terminal Region of the Single-Stranded DNA Binding Protein of Herpes Simplex Virus Type 1 Is Required for Cooperative DNA Binding
... the protein without being directly involved in contacts with nucleotides ...mediate protein-nucleic acid contacts via stacking and electrostatic ...DNA binding and that the fluores- cence of ... See full document
11
DNA-binding protein associated with herpes simplex virus DNA polymerase.
... Again, the protein was eluted by a gradient of 0 to 0.4 M KCI, and the fractions indicated by the solid horizontal line which were found to contain ICSP 34, 35 were pooled, dialyzed, and[r] ... See full document
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