[PDF] Top 20 Herpes Simplex Virus and Human Cytomegalovirus Replication in WI-38 Cells I. Sequence of Viral Replication

... This was true even in late stages of Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Appearance of increased numbers of perichromatinlike granules Condensation of the c[r] ... See full document

... late viral proteins by inhibiting cellular or viral factors needed for their ...recombinant virus at an MOI of 10 and treated with 50 ␮ M OSMI-1 or DMSO. Cells were fixed at 8 hpi, stained ... See full document

... various viral infections. Since viral infection is a type of cell stress, p53’s effects can reduce replication of some viruses, and, therefore, these viruses have ac- quired mechanisms to counteract ... See full document

... HEK293T cells were transfected with pcDNA-FEM- UL51 using polyethylenimine as described previously ...RefSeq human protein database (National Center for Biotechnology Information [NCBI]) and the 74 ... See full document

... (iii) UL8 constructs. The UL8 ORF was isolated on an EcoRI-to-XbaI frag- ment from pCM-UL8 (17) and inserted between the same two sites in pGEM- 7Zf 1 (Promega, Madison, Wis.). The resulting construct was designated ... See full document

... (34). Virus dl1403/CMVlacZ is a derivative of dl1403 that contains the lacZ gene under the control of the HCMV promoter/enhancer inserted into the tk gene was a gift from Chris ...strain. Human fibroblast ... See full document

... robust replication in tumor cells, suggests that ΔN146 is a unique oncolytic ...EUs11 virus, which mediates an immunosuppressive ...gene I, or melanoma differentiation-associated gene 5, which ... See full document

... polymerase I and then treated with ...transfected cells were plated, puromycin was added 24 h later, and after an additional 24 h during which puromycin-sensitive cells were killed, the drug was ... See full document

... A herpes simplex virus (HSV), ...constructing replication- competent controlled viruses for several reasons. The virus has a double-stranded DNA genome of ⬎150 kbp that tolerates large ... See full document

... with herpes simplex virus type 1 (HSV-1) results in the rapid elimination of mitochondrial DNA (mtDNA) from host ...the viral alkaline nuclease (UL12) called ...on viral ... See full document

... of viral mRNA splicing in infected cells. The viral RNAs detected about 12 h after infection are the 2-kb, multiply spliced mRNAs that encode the regulatory proteins ...first viral proteins ... See full document

... Cytochemical Localization of Lysosomal Enzymes in Infected Cells JANET DUYCKINCK SMITH"* AND ETIENNE DE HARVEN2 Department of Anatomy, The Medical College of Pennsylvania and Hospital, P[r] ... See full document

... Fusion appeared to begin at one or more small foci of contact between the viral envelope and the plasmalemma, and to proceed very rapidly, since naked viral capsids free in the cytoplasm[r] ... See full document

... acid sequence similarity was observed between some US12 family ORFs and the BAX Inhibitor-1 (BI-1) protein, which modulates apoptotic responses ...affect viral replication in fi- broblasts, these ... See full document

... BHK cells (lanes 1 through 3 and 16 through 18) and MeWO cells (lanes 4, 8 through 11, and ...MeWO cells treated with ACV, PAA, and ara-C (lanes 5 through 7 and 12 through 14, ... See full document

... in1814-infected cells in that report were made difficult because of the very high back- ground (mock-infected) levels of JNK ...in1814-infected cells may contribute to the failure to detect JNK activation ... See full document

... blocking viral replication than treatment later in ...decrease viral entry when added to virions or to cells to which virions had been allowed to attach ...in viral yield when the ... See full document

... for viral replication? Although the Exo III mutations reduced exonu- clease activity at least 50-fold, they were not ...and virus yield (Table 4) in Vero ...on replication in transient ... See full document

... that human cytomegalovirus (HCMV) infection of fibroblasts markedly affects p53 and other regulatory proteins and inhibits transit through the cell cycle ...infected cells during the first 24 h ... See full document

... Viral true late gene transcription is not inhibited during G207 infection of GSCs. We next examined virus transcription, which is kinetically regulated. Quantitative reverse transcription-PCR (qRT-PCR) ... See full document