[PDF] Top 20 Herpes simplex virus type 1 gene products required for DNA replication: identification and overexpression.

... These data are consistent with the view that the genes ULS, UL8, UL9, UL42, and ULS2, like the genes encoding ICP8 and polymerase 12, 15, are all delayed-early genes, as expected for our[r] ... See full document

... Herpes simplex virus (HSV) gene expression during the course of in vitro infection has been well characterized (5, 6, 20, ...These gene products, in turn, upregulate the ... See full document

... 1,000-bp DNA fragments. Protein-DNA complexes were immunoprecipitated using 5 ␮g of antibodies against histone H3 (ab1791; ...cellular DNA fragments in the immunoprecipitated material was analyzed by ... See full document

... of products by CsCl density gradient sedimentation, the dTTP in the reaction mixture was replaced by bromo-dUTP (BrdUTP) ...Deproteinized replication products were loaded onto a solution of 3 ml of ... See full document

... Identification of the Oscar elements. As an alternative means of identifying specific flanking sequences that can enhance the function of the minimum core origin contained in pOS 80 (80), oriS-flanking sequences ... See full document

... CHALLBERG2* Medical Research Council Virology Unit, Institute of Virology, University of Glasgow, Church Street, Glasgow GIl 5JR, United Kingdom,' and Laboratory of Viral Diseases, Natio[r] ... See full document

... is required for inhibition of HSV-1 rep- ...FIG 1 Replication of HSV-1 is enhanced in SAMHD1-depleted ...(C) DNA was extracted at the indicated time points from differentiated ... See full document

... To isolate and propagate this mutant, we generated a cell line, BL-1, by cotransfection of Vero cells with pSV2neo and a plasmid containing the herpes simplex virus type 1 KOS strain Bam[r] ... See full document

... delocalization of nucleolin and for the formation of nucleolin granular dots, we analyzed the nucleolin localization by immu- nofluorescence in cells infected with HSV-1 strains defective in specific viral genes. ... See full document

... Assessment of the latent viral DNA burden by quantitative PCR QPCR demonstrated a reduction in the number of copies of the Ung- mutant viral Downloaded from http://jvi.asm.org/ on Novemb[r] ... See full document

... h. Virus capsids were isolated and purified from nuclear lysates of infected cells on a 20 to 50% sucrose gradient, essentially as described previously ...7.4], 1 mM ... See full document

... supF gene, the amp gene, and the colE1 sequences; this replicon was inserted into the PstI site of the tk gene of ...infectious virus DNA and SpeI-linearized pSupF-tk-R into Vero ... See full document

... 8lacZ replication to an extent similar to that of wild-type ICP8 ...the replication of an ICP8 mutant ...8lacZ replication can be attributed entirely to the D1087A ...is required for ... See full document

... UL56 gene product of herpes simplex virus (HSV) has been shown to play an important role in viral ...HSV type 2 (HSV-2) and examined its expression and properties. The gene ... See full document

... nucleus required coexpres- sion of all of its subunits ...in replication compartments. Within the large globular replication compartments, substructures can be seen as previously reported for UL29 ... See full document

... Herpes simplex virus type 1 (HSV-1) DNA replication intermediates exist in a complex nonlinear structure that does not migrate into a pulsed-field ...UL12 ... See full document

... Identification and abundance of UL6, UL17, and UL25 pro- teins in B capsids. To obtain information on the identity and relative abundance of the capsid-associated packaging pro- teins, the proteins of B capsids of ... See full document

... Herpes simplex virus type 1 (HSV-1) replication produces large intracellular DNA molecules that appear to be in a head-to-tail concatemeric ...these DNA ... See full document

... Adeno-associated virus type 2 (AAV), a member of the parvovirus family, contains a single-stranded genome of 4,679 ...the replication (Rep) proteins and the other coding for the structural ... See full document

... UL25 gene. The 580-amino-acid open reading frame for the UL25 gene extends from nucleo- tides 48813 (ATG) to 50553 (TAG) in the HSV-1 genome ...The products of the UL26 and ...UL25 gene ... See full document