[PDF] Top 20 Herpes simplex virus type 1 recombination: role of DNA replication and viral a sequences.

... 277 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest During the course of infection, elements of the herpes simplex virus type 1 HSV-1 genome undergo inversion, a proces[r] ... See full document

... The herpes simplex virus type 1 (HSV-1) origin of DNA replication, oriS, contains three binding sites for the viral origin binding protein (OBP) flanked by ... See full document

... quantitative recombination assay originally developed to assess the role of the a sequences in HSV-1 genome isomerization ...out DNA inversion-type ...of replication and ... See full document

... of herpes simplex virus type 1 (HSV-1) DNA replication during productive infection of fibroblasts and epithelial cells requires attachment of the origin binding ... See full document

... Herpes simplex virus type 1 (HSV-1) DNA replication intermediates exist in a complex nonlinear structure that does not migrate into a pulsed-field ...a role ... See full document

... a virus lacking the polymerase, but they do not contain ...to replication foci. However, in cells infected with a UL42 null virus, PML was detected in viral foci in some but not all of the ... See full document

... American Type Culture Collection and were propagated in Dulbecco modified Eagle minimum essential medium supplemented with 10% fetal calf serum, ...of recombination in COS cells were constructed by addition ... See full document

... Assessment of the latent viral DNA burden by quantitative PCR QPCR demonstrated a reduction in the number of copies of the Ung- mutant viral Downloaded from http://jvi.asm.org/ on Novemb[r] ... See full document

... lytic replication, a VP19C NES-mutated recombinant virus was ...Red-mediated recombination system in E. coli GS1783 strain using a HSV-1 17-37BAC ...Figure 1. Primer sequences ... See full document

... of viral progeny ...its role in the infectious process of HSV is currently ...following virus infection, and the significance of these induc- tions is also unknown (20, 71, 81, ... See full document

... The open reading frames for the UL25, UL6, and UL9 genes were cloned into the BamHI site of the baculovirus transfer vector pVL941 as follows. pAC-UL25 was constructed by digesting pSG18 (21) with BsrI, the ends were ... See full document

... The other isolate, SP22-4, carried a 3.5-kilobase-pair duplication defined by a recombination event between a tandem repeat array in the BamHI Z fragment and a site near the amino termin[r] ... See full document

... of viral DNA during HSV-1(F) infection was lower in cdc25C ⴚ / ⴚ cells than in cdc25C ⴙ / ⴙ MEF ...a role in HSV-1 DNA ...a role in viral DNA accumulation, ... See full document

... FIG 1 Replication of HSV-1 is enhanced in SAMHD1-depleted ...(C) DNA was extracted at the indicated time points from differentiated THP-1 cells infected with HSV-1 at an MOI of ... See full document

... Herpes simplex virus type 1 (HSV-1) replication produces large intracellular DNA molecules that appear to be in a head-to-tail concatemeric ...these DNA ... See full document

... nonhomologous recombination contrib- uted significantly to the high frequency of recombination de- tected in our ...recombined DNA revealed that the majority of these IM-HR events resulted in ... See full document

... full-length DNA chains that are essentially free of nicks ...of replication products migrates in the first di- mension at a position consistent with their being mAAV ...ITR sequences that would ... See full document

... In order to investigate the role of transactivation by ICPO in the de novo synthesis of infectious virus and to identify the intramolecular functional domains responsible for this activi[r] ... See full document

... herpesvirus DNA. By introducing single copies of test packaging signals into an HSV-1 genome lacking the normal resident copies, we have overcome one of the major drawbacks of the previously used amplicon ... See full document

... with herpes simplex virus type 1 (HSV-1) results in the rapid elimination of mitochondrial DNA (mtDNA) from host ...the viral alkaline nuclease (UL12) called ...on ... See full document