[PDF] Top 20 1 High-throughput direct mass spectrometry-based
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1 High-throughput direct mass spectrometry-based
... regional brain alterations in the APP/PS1 transgenic model of Alzheimer’s disease by direct infusion mass 308. spectrometry[r] ... See full document
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Towards high throughput and spatiotemporal proteomics : analytical workflows and quantitative label-free mass spectrometry
... are based on different physicochemical properties and were performed at different levels – at the protein level with SDS-PAGE and at the peptide level with SCX and ...total mass spectrometry analysis ... See full document
167
A SENSITIVE, HIGH THROUGHPUT ESTIMATION OF METHOTREXATE IN HUMAN PLASMA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY
... Several methods have been reported for the estimation of methotrexate from plasma. Dan Wu et al., 22 developed a method for determination of methotrexate in human plasma using liquid chromatography tandem mass ... See full document
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High throughput discovery of thermo responsive materials using water contact angle measurements and time of flight secondary ion mass spectrometry
... materials based upon the thermal release of attached eukaryotic ...a direct screen of water contact angle switching to identify thermo-responsive materials rather than implying switchability through cell ... See full document
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High-throughput analysis of amino acids in plant materials by single quadrupole mass spectrometry
... methodology based on ion exchange chromatography and involving very sensitive pH adjustments as well as chromatographic run times of well above 1 h per injection ...methods based on ESI-TOF MS [9], ... See full document
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A SIMPLE, SENSITIVE, SELECTIVE AND HIGH THROUGHPUT LIQUIDCHROMATOGRAPHY/TANDEM MASS SPECTROMETRY (LC/MS/MS)ASSAY OF EXEMESTANEIN HUMAN PLASMA
... 3.2 Assay performance and validation Throughout the method validation, the precision (%CV) of the system suitability test was observed ≤ 1.86%for analyte RT, IS RT and Area ratio of analyte andIS, whilethe signal to ... See full document
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Proteomic profiling of retinoblastoma by high resolution mass spectrometry
... siRNA based knock- down study of CRABP2 in head and neck squamous cell carcinoma showed decrease in tumour cell invasion ...(1–14) based on their phylogenetic analysis of motor ... See full document
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Investigating protein structure by means of mass spectrometry
... charge-to-surface ratio. At a critical limit, the tip of the cone extends into a filament and highly-charged droplets are released from it (Kebarle 2000). The production of droplets is aided by a nebulising gas (usually ... See full document
231
Mass spectrometry of planetary exospheres at high relative velocity: direct comparison of open- and closed-source measurements
... area normalised to 10 −9 mbar versus azimuthal rotation an- gle is shown. In the upper left panel a of Fig. 7, the n-mode area is plotted for different species in the measured gas mix- tures. The solid lines give the ... See full document
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Mass spectrometry imaging of cassette dosed drugs for higher throughput pharmacokinetic and biodistribution analysis
... of mass spectra at discrete points on a tissue section, have recently emerged as technologies that have the capability to obtain label-free compound biodistribution, relative abundance and quantitation data by ... See full document
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Development and Validation of Extraction Method for the Determination of UC781 in Cervicovaginal Fluid
... separation. Based on this extraction method, we validated a reverse phase high performance liquid chromatography with electrospray ionization mass spectrometry assay in order to quantify UC781 ... See full document
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Simultaneous untargeted and targeted metabolomics profiling of underivatized primary metabolites in sulfur-deficient barley by ultra-high performance liquid chromatography-quadrupole/time-of-flight mass spectrometry
... crop), high-throughput methods need to be ...The high polarity and acid–base properties implies time consuming sample preparation ...methods based on LC–MS have been reported in the literature ... See full document
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Quantification in untargeted mass spectrometry-based metabolomics
... in high-throughput whole organism pre- clinical drug screening and toxicology studies as less drug is required, a larger number of animals can be used and less ethical issues are associated [ 97 , 121 ... See full document
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Functional phosphoproteomic mass spectrometry-based approaches
... allow high- throughput analysis or imply very labour intense operations ...that high- throughput analysis of phosphorylated protein residues can be developed ... See full document
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A modified FASP protocol for high-throughput preparation of protein samples for mass spectrometry
... Proteins in lysis buffer were reduced by the addition of 0.5 M DTT stock solution to a final concentration of 20 mM followed by incubation at 95˚C for 5 min and cooling at room tem- perature (rt) for 10 min. Proteins ... See full document
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High Resolution Genotyping of Campylobacter Species by Use of PCR and High Throughput Mass Spectrometry
... MLST strain typing on the T5000 platform and clonal com- plex association for primer selection. The assay for Campy- lobacter on the T5000 platform incorporates two primer pairs that unambiguously distinguish C. coli and ... See full document
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High-throughput profiling of small molecules using mass spectrometry
... Studies in zebrafish larvae are increasingly used for modeling human diseases with the ex- pectation that they will be able to contribute towards bridging the gap between in vitro cell based assays and in vivo ... See full document
109
Comparative proteomic analysis of normal and tumor stromal cells by tissue on chip based mass spectrometry (toc-MS)
... sufficient high sensitivity to analyse these small amounts of cells on the other ...but mass spectrome- try by MALDI (matrix assisted laser desorption and ionization) seems to be a better choice as shown in ... See full document
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Examination of tumour tissues by direct MALDI mass spectrometry imaging and profiling
... Tissue- based proteomics is becoming an attractive research field as it provides opportunities for biomarker discovery related to disease state, hence improving early detection and ...are based on the use ... See full document
281
Discovering human protein diversity
... yet, mass spectrometry is only a method of detection, and as with any other detection tech- nique, the separation processes that prepare the proteins for MS detection are ...with mass ... See full document
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