[PDF] Top 20 New Highly Sensitive Real Time PCR Assay for HIV 2 Group A and Group B DNA Quantification
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New Highly Sensitive Real Time PCR Assay for HIV 2 Group A and Group B DNA Quantification
... ABSTRACT HIV-2 infection is characterized by a very low replication rate in most cases and low ...for HIV-1 infection. Here, a new highly specific and sensitive method for ... See full document
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Development and Application of Real Time PCR Assay for Quantification of Mycobacterium ulcerans DNA
... TaqMan assay may help to answer the question of how wide surgical excision has to be to avoid ...and sensitive enumeration of AFB in ZN- stained tissue ...able quantification method, since ... See full document
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A New Highly Sensitive and Specific Real Time PCR Assay Targeting the Malate Dehydrogenase Gene of Kingella kingae and Application to 201 Pediatric Clinical Specimens
... In contrast, RTX-targeting RT-PCR assays have gained great popularity worldwide because they were initially believed to be highly specific for K. kingae (16, 17). However, the RTX locus of K. kingae is ... See full document
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Quantification of Leishmania infantum DNA by a Real Time PCR Assay with High Sensitivity
... less sensitive than a kinetoplast DNA-based ...Leishmania DNA by targeting a repeated genomic sequence, and this method reached a sensi- tivity of 1 parasite per reaction; this assay was ... See full document
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A highly sensitive, non invasive qPCR based strategy for direct quantification of Yersinia ruckeri in fish faeces
... A real-time polymerase chain reaction assay for identification and quantification of Flavobacterium psychrophilum and application to disease resistance studies in selectively bred rainbow ... See full document
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Novel Light Upon Extension Real Time PCR Assay for Simultaneous Detection, Quantification, and Genogrouping of Group A Rotavirus
... reference DNA (5 ...our assay. In an earlier study, we described a real-time PCR assay for detection and differentiation between norovirus genogroups I and II, using the same LUX ... See full document
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Simultaneous Genotyping and Quantification of Hepatitis B Virus for Genotypes B and C by Real Time PCR Assay
... this new method for quantification was compared with that of a commercial assay with good statistical ...GQ-PCR assay and that obtained by direct sequencing (Table ...GQ-PCR ... See full document
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Quantification of DNA in Plasma by an Automated Real Time PCR Assay (Cytomegalovirus PCR Kit) for Surveillance of Active Cytomegalovirus Infection and Guidance of Preemptive Therapy for Allogeneic Hematopoietic Stem Cell Transplant Recipients
... antigenemia assay has been adopted by many transplant centers as the method of choice for guiding preemptive therapy in Allo-SCT ...positive PCR tests performed on whole blood has also been shown to be a ... See full document
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Performance of the Cobas AmpliPrep/Cobas TaqMan Real Time PCR Assay for Hepatitis B Virus DNA Quantification
... HBV DNA levels in international units per milliliter), automated, and rapid, with minimal hands-on time ...HBV DNA, regardless of the HBV genotype or sequence polymor- ...HBV DNA assays based ... See full document
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Characterization of a New Sensitive PCR Assay for Quantification of Viral DNA Isolated from Patients with Hepatitis B Virus Infections
... CAM assay, an end point PCR method, the technology based on real-time PCR amplification offers many advantages, in particular a wider range of ...conventional PCR assays, this ... See full document
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A New Real Time Quantitative PCR for Diagnosis and Monitoring of HIV 1 Group O Infection
... the PCR products after each cycle. PCR was performed with the RNA Ultrasense One-Step Quantitative RT-PCR kit (Invitrogen, Cergy Pon- toise, ...for 2 min (1 cycle), followed by denaturation at ... See full document
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Higher Specificity of Nucleic Acid Sequence Based Amplification Isothermal Technology than of Real Time PCR for Quantification of HIV 1 RNA on Dried Blood Spots
... the HIV-1 RNA level (viral load [VL]) in resource-limited ...cell-associated HIV-1 DNA during reverse transcription (RT)-PCR can be avoided by using nucleic acid sequence-based amplification ... See full document
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Performance of Version 2 0 of the Cobas AmpliPrep/Cobas TaqMan Real Time PCR Assay for Hepatitis B Virus DNA Quantification
... Sanguine. Group A comprised 103 HBV-seronegative individuals (with no markers of past or ongoing HBV infec- tion); group B comprised 97 patients with serological profiles of resolved HBV infection ... See full document
7
Real time PCR assays for hepatitis B virus DNA quantification may require two different targets
... and group B consisted of 59 plasma samples obtained from patients at Beijing ChaoYang Hospital between July 2016 and Au- gust ...2016. Group C was 75 HBV DNA positive plasma samples obtained ... See full document
9
Multicenter Performance Evaluation of a New TaqMan PCR Assay for Monitoring Human Immunodeficiency Virus RNA Load
... AMPLICOR HIV-1 MONITOR (CAHIM) test ...transcriptase PCR assay (3), has been widely used in research as well as in clinical ...practice. Quantification of HIV-1 RNA with this ... See full document
5
Highly sensitive detection of the group A Rotavirus using Apolipoprotein H coated ELISA plates compared to quantitative real time PCR
... same time detec- tion and discrimination on one plate and in one ...a new method for the specific and sensitive detection of viruses directly from stool samples by a poly-specific binding of antigen ... See full document
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A Genotype Independent Real Time PCR Assay for Quantification of Hepatitis B Virus DNA
... There are limitations with our in-house assays. The lack of an internal control does not allow us to rule out false-negative results due to the presence of inhibitors to PCR amplification. The limit of detection ... See full document
6
New Sensitive One Step Real Time Duplex PCR Method for Group A and B HIV 2 RNA Load
... current assay, the new test has a significantly better analytical limit of quantification, reaching 50 cp/ml with manual extraction of 200 l of plasma and 40 cp/ml with auto- mated extraction of 1 ... See full document
6
Development of a new ultra sensitive real-time PCR assay (ultra sensitive RTQ-PCR) for the quantification of HBV-DNA
... HBV- DNA (OptiQuant® HBV-DNA Quantification Panel, Accrometrix Europe ...the assay were ...ultra sensitive real-time PCR was tested similar (67%) to the Procleix ... See full document
6
Development of a new ultra sensitive real time PCR assay (ultra sensitive RTQ PCR) for the quantification of HBV DNA
... HBV- DNA quantifications using the two in house real-time PCR assays and COBAS TaqMan as ...(ultra sensitive RTQ-PCR vs COBAS TaqMan HBV Test) the fitted regression line was ... See full document
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