[PDF] Top 20 Human immunodeficiency virus type 1 integrase: effect on viral replication of mutations at highly conserved residues.
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Human immunodeficiency virus type 1 integrase: effect on viral replication of mutations at highly conserved residues.
... The role of these residues in IN function has been investigated in vitro, using deletion and substitution mutants of IN protein from human immunodeficiency virus type 1 HIV-1 11, 14, 30,[r] ... See full document
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The highly conserved aspartic acid residue between hypervariable regions 1 and 2 of human immunodeficiency virus type 1 gp120 is important for early stages of virus replication.
... involving residues 131 and 157 (Fig. 1). Between residues 119 and 205, there are about 90 amino acid residues, which account for approximately one-fifth of the gp120 coding ...Functionally, ... See full document
5
Human immunodeficiency virus type 1 integrase: effects of mutations on viral ability to integrate, direct viral gene expression from unintegrated viral DNA templates, and sustain viral propagation in primary cells.
... only viral protein necessary for integration of retroviral DNA into chromosomal DNA of the host ...of human immunodeficiency virus type 1 (HIV-1) integrase with ... See full document
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Multiple effects of mutations in human immunodeficiency virus type 1 integrase on viral replication.
... the human immunodeficiency virus type 1 (HIV-1) genome into a chromosome of an infected cell is a pivotal step in virus ...the virus-encoded integrase, which ... See full document
8
Formation of a Human Immunodeficiency Virus Type 1 Core of Optimal Stability Is Crucial for Viral Replication
... wild- type HIV-1 (von Schwedler and Sundquist, ...These residues are all highly conserved in HIV-1 isolates from HIV-infected ...the mutations did not impair cone ... See full document
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Irreversible Inhibition of Human Immunodeficiency Virus Type 1 Integrase by Dicaffeoylquinic Acids
... the replication of retroviruses and (ii) integrase has no known functional analogue in human cells (for reviews, see references 20 and ...the viral enzyme. The major classes of ... See full document
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Resistance Mutations in Human Immunodeficiency Virus Type 1 Integrase Selected with Elvitegravir Confer Reduced Susceptibility to a Wide Range of Integrase Inhibitors
... Genotyping. Viral RNA was extracted from culture supernatant or virus stock using a NucliSens easyMAG apparatus (bioMe ´rieux, Marcy l’ Etoile, France), a high-throughput automated nucleic acid extraction ... See full document
9
Inhibition of Human Immunodeficiency Virus Type 1 Replication with Artificial Transcription Factors Targeting the Highly Conserved Primer-Binding Site
... integrated viral genome. The HIV-1 5 ⬘ long terminal repeat (LTR) contains binding sites for a number of host transcription factors, including Sp1, NF- B, and Lef-1 ...inactivated. Highly ... See full document
11
Proline Residues in Human Immunodeficiency Virus Type 1 p6Gag Exert a Cell Type-Dependent Effect on Viral Replication and Virion Incorporation of Pol Proteins
... the viral pellets were dissolved in 25 ml of RT lysis buffer (1% Triton X-100, 20 mM Tris-HCl [pH ...KCl, 1 mM dithiothreitol, 30% glycerol), and 10 ml of each sample was used for the RT ...reaction. ... See full document
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Effect of Polypurine Tract (PPT) Mutations on Human Immunodeficiency Virus Type 1 Replication: a Virus with a Completely Randomized PPT Retains Low Infectivity
... a virus that lacks a PPT could produce sufficient Tat protein to activate the reporter gene in these cells during ...nonintegrated viral DNA, particularly in our study, from the increased number of 2-LTR ... See full document
9
Protective HLA Class I Alleles That Restrict Acute-Phase CD8+ T-Cell Responses Are Associated with Viral Escape Mutations Located in Highly Conserved Regions of Human Immunodeficiency Virus Type 1
... acid residues across HIV-1 (see Table S2 in the supplemental ...HLA-associated mutations were then categorized into four classes based on their location relative to epitopes: (i) located within ... See full document
11
Mutations in the Human Immunodeficiency Virus Type 1 Integrase D,D(35)E Motif Do Not Eliminate Provirus Formation
... with virus encoding wild-type IN ...integrated viral DNA in cells infected with virus encoding mutations at each of the three residues of the D,D(35)E ...integrated viral ... See full document
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Human immunodeficiency virus type 1 integrase mutants retain in vitro integrase activity yet fail to integrate viral DNA efficiently during infection.
... or viral DNA ...infecting virus demonstrates effi- cient synthesis of linear viral DNA ...integrate viral DNA (Table ...defect. Mutations R199C and W235E may alter the architecture of ... See full document
8
Lys-34, Dispensable for Integrase Catalysis, Is Required for Preintegration Complex Function and Human Immunodeficiency Virus Type 1 Replication
... the viral integrase (IN) acting upon the viral attachment (att) sites at the ends of linear viral cDNA within the context of a large nucleoprotein complex referred to as the preintegration ... See full document
8
Nef Enhances Human Immunodeficiency Virus Type 1 Infectivity and Replication Independently of Viral Coreceptor Tropism
... efficient replication and pathogenicity of human and simian immodeficiency viruses (HIV and SIV, respec- tively) (15, 25, ...increases viral spread both indirectly by mechanisms of immune evasion and ... See full document
5
Targeting Human Immunodeficiency Virus (HIV) Type 2 Integrase Protein into HIV Type 1
... other viral and cellular proteins that make up the viral nucleo- protein complex ...the virus life cycle. Host cell proteins that bind to IN or the HIV-1 preintegration complex (PIC) and ... See full document
6
Disulfide-Linked Integrase Oligomers Involving C280 Residues Are Formed In Vitro and In Vivo but Are Not Essential for Human Immunodeficiency Virus Replication
... The human immunodeficiency virus type 1 integrase (IN) forms an oligomer that integrates both ends of the viral ...which residues are responsible for the disulfide ... See full document
7
Genetic Analyses of Conserved Residues in the Carboxyl-Terminal Domain of Human Immunodeficiency Virus Type 1 Integrase
... of 1% bovine serum albumin and precleared with 50 l protein A-Sepharose CL-4B beads (Amer- sham Pharmacia Biotech AB, Uppsala, Sweden) hydrated in phosphate-buffered ...saline. Viral proteins were ... See full document
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Conserved sequences in the carboxyl terminus of integrase that are essential for human immunodeficiency virus type 1 replication.
... of human immunodeficiency virus type 1 integrase (HIV-1 IN), W-235, the requirement for which is only revealed in viral assays for integrase function ...are ... See full document
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Posttranslational Acetylation of the Human Immunodeficiency Virus Type 1 Integrase Carboxyl-Terminal Domain Is Dispensable for Viral Replication
... additional viral mutants and measured the accumulation of late RT products and two-long- terminal-repeat (2-LTR) circles, as well as integration fre- quency and syncytium formation (Table ...an integrase ... See full document
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