[PDF] Top 20 A Hypothesis for DNA Viruses as the Origin of Eukaryotic Replication Proteins

... incomplete proteins, the remain- ing sequences were aligned using ClustalW to aid in identification of homologous ...in DNA Pol alpha by Wang et ...1 DNA Pol in the study to T4 DNA Pol by ... See full document

... viral replication cy- cles or by origin from a common viral ...homologous proteins of viruses from different families show moderate or weak sequence conserva- tion and may be less similar to ... See full document

... Various DNA viruses are known to cause severe infectious diseases in both plants and mammals, including humans ...of DNA virus replication is well con- served among plants and mammals ...viral ... See full document

... known proteins with these characteristics suggested that the cellular factor might be a member of the high-mobility group of small, nonhistone chromosomal proteins, which bind tightly to phosphocellulose ... See full document

... an origin of replication ...[23]. Origin activation is therefore irreversible once the cell becomes committed to the cell cycle after passing the restriction ...the origin leads to the action ... See full document

... well-coordinated DNA replica- tion in S phase and separation of duplicated chromo- somes into daughter cells in mitosis ...the DNA repli- cation will initiate, is assembled ...conserved replication ... See full document

... the hypothesis that cruciform extrusion provides the ssDNA con- formation necessary for cleavage in vivo, as in the cases of the plasmids of the pT181 family (24, ...viral origin of replication by an ... See full document

... positive-strand DNA synthesis requires cleav- age at a specific site in the positive-strand ...C1 proteins (26, ...for DNA cleavage by the TYLCV C1 protein ...vivo replication results showing ... See full document

... no replication protein in CAV has been characterized to date, and our analysis of the three proteins described earlier (21) did not reveal extensive homology to the putative rep protein of ...rep ... See full document

... this hypothesis, we created three additional ...the DNA-binding domain of GAL4 (5, ...transient replication assay, despite the fact that cellular Sp1 or, in the case of the ...mediated DNA ... See full document

... In addition to the diverse lines of evidence that are com- patible with the inside-out theory, it is worth highlighting that the inside-out model involves a simple series of steps, each of which draws upon plausible ... See full document

... To analyze whether the left-hand AAV fragment containing the AAV-2 origin/TR structure is able to promote replication activity in carcinogen-treated cells in the absence of parvoviral ge[r] ... See full document

... By inhibiting DNA synthesis, this signal sequence would ensure that the plus strand palindrome remains free to renature as a foldback structure when initiation and rightward chain extens[r] ... See full document

... sequences, roughly 80 bp in Py around the PvuII site at nt 5130, required for replication 14a, 24, and the similar SV40 replication-enhancing two 21-bp repeats 1; an AT-rich cluster nece[r] ... See full document

... The replication ability of the four virus strains were assessed separately in both ...H9N2 viruses were found to grow efficiently in ECEs and cell ...culture-grown viruses exhibited 2-3 fold lower ... See full document

... To more precisely delineate the boundaries of the polyomavirus ori we constructed a series of mutants that contain progressive deletions within viral DNA sequences originating from eithe[r] ... See full document

... The results obtained from the deletion analysis of the 3' terminal region of pregenomic RNA demonstrate that WHV sequences from position 18 to the polyadenylation signal on pregenomic RN[r] ... See full document

... Recently, rhesus macaque rhadinovirus (RRV), the rhesus macaque homolog of human herpesvirus 8 (HHV-8), or Ka- posi’s sarcoma-associated herpesvirus, was isolated from a sim- ian immunodeficiency virus-infected animal ... See full document

... Previous in vitro replication studies with either nuclear extracts prepared from cells infected by Ad2tslll or purified Ad5ts125 DBP have shown that those proteins are temperature sensit[r] ... See full document

... SPSF I and II specifically bind to single-stranded oligonucleotides containing sequences derived from one strand of the BPV-1 minimal origin of replication, from either strand of domain [r] ... See full document